Favorite composite part——BNP-AR185 T2A eGFP-polyA

For this year’s iGEM competition, our favorite composite part is B185, short for BNP-AR185-polyA, This part is composed of BNP promotor, AR185 linked with eGFP by T2A and poly A.

Figure 1.The B185 construct

How B185 works

As has shown presiously, BNP promoter activity is related to the severity of heart failure. So we want to use ET-1, one stimulus factor, to imitate the environment of heart failure and induce BNP promoter expression. Then, the later sequence of the part, AR185, also can be expressed and fuction what it could specifically bind to RyR2 in rat cardiomyocytes and have the ability to inhibit PKA dependent S2808 phosphorylation in vitro.

Figure 2.Design and Function of Ca²⁺RTIN

See More

Integrated information about AR185 and BNP promotor posted on Demonstrate page. Or you can alternatively check the (BBa_I712008) on the Registry of Standard Biological Parts website.

We listed other composite part we constructed this year below.

References

1. Sergeeva, I.A. and V.M. Christoffels, Regulation of expression of atrial and brain natriuretic peptide, biomarkers for heart development and disease. Biochim Biophys Acta, 2013. 1832(12): p. 2403-13.
2. Bingham, A.J., et al., The repressor element 1-silencing transcription factor regulates heart-specific gene expression using multiple chromatin-modifying complexes. Mol Cell Biol, 2007. 27(11): p. 4082-92.
3. Kerkela, R., et al., Key roles of endothelin-1 and p38 MAPK in the regulation of atrial stretch response. Am J Physiol Regul Integr Comp Physiol, 2011. 300(1): p. R140-9.
4. Lu, Y.M., et al., DY-9760e inhibits endothelin-1-induced cardiomyocyte hypertrophy through inhibition of CaMKII and ERK activities. Cardiovasc Ther, 2009. 27(1): p. 17-27.