Difference between revisions of "Template:Virginia/Basic Part"

Line 1: Line 1:
−
<h1 id="description">Description</h1>
+
<h1 id="basic-parts">Basic Parts</h1>
−
<p>Lorem ipsum dolor, sit amet consectetur adipisicing elit. Asperiores consequuntur modi maiores blanditiis aperiam, hic molestias, doloremque aliquid dolorum temporibus eum a earum architecto dignissimos odit porro dolor! Earum, aspernatur.</p>
+
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Name</th>
 +
        <th>Description</th>
 +
        <th>Part</th>
 +
    </tr>
 +
    <tr>
 +
        <td>spacerLXf</td>
 +
        <td>This is a 10 nucleotide spacer used to build BBa_K2535005. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won&#39;t be assembled in the wrong place. </td>
 +
        <td> K2535003</td>
 +
    </tr>
 +
    <tr>
 +
        <td>spacerLXr </td>
 +
        <td>This is a 10 nucleotide spacer used to build BBa_K2535005. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won&#39;t be assembled in the wrong place. </td>
 +
        <td>K2535004</td>
 +
    </tr>
 +
    <tr>
 +
        <td>spacerLRf </td>
 +
        <td>This is a 10 nucleotide spacer used to build BBa_K2535009. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won&#39;t be assembled in the wrong place. </td>
 +
        <td>K2535007</td>
 +
    </tr>
 +
    <tr>
 +
        <td>spacerLRr </td>
 +
        <td>This is a 10 nucleotide spacer used to build BBa_K2535009. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won&#39;t be assembled in the wrong place.</td>
 +
        <td>K2535008 </td>
 +
    </tr>
 +
    <tr>
 +
        <td>spacerYf </td>
 +
        <td>This is a 10 nucleotide spacer used to build BBa_K2535013. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won&#39;t be assembled in the wrong place.</td>
 +
        <td> K2535011</td>
 +
    </tr>
 +
    <tr>
 +
        <td>spacerYr</td>
 +
        <td>This is a 10 nucleotide spacer used to build BBa_K2535013. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won&#39;t be assembled in the wrong place.</td>
 +
        <td>K2535012</td>
 +
    </tr>
 +
    <tr>
 +
        <td>YdgG </td>
 +
        <td> YdgG is a membrane transport protein, not native to the Lsr Operon, which exports AI-2 out of the cell (Herzberg et al 2006).</td>
 +
        <td> BBa_K2535002</td>
 +
    </tr>
 +
    <tr>
 +
        <td>pLsr_rbs_LsrR_rbs </td>
 +
        <td>This part is an AI-2 (autoinducer 2 from the Lsr Operon) dependent promoter that self regulates itself. pLsr is the specific promoter that is bidirectional. Dimers of the LsrR gene bind to and repress this promoter. However, AI-2P (phosphorylated AI-2) can bind to these dimers and cause them to dissociate, depressing the promoter.  Since the expression of this plasmid produces LsrR, which represses the promoter pLsr, this system is self regulating. </td>
 +
        <td>K2535018</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h1 id="references">References</h1>
 +
<p>Gonzalez (2006)<br>Herzberg et al (2006)  </p>

Revision as of 05:30, 14 October 2018

Basic Parts

Name Description Part
spacerLXf This is a 10 nucleotide spacer used to build BBa_K2535005. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won't be assembled in the wrong place. K2535003
spacerLXr This is a 10 nucleotide spacer used to build BBa_K2535005. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won't be assembled in the wrong place. K2535004
spacerLRf This is a 10 nucleotide spacer used to build BBa_K2535009. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won't be assembled in the wrong place. K2535007
spacerLRr This is a 10 nucleotide spacer used to build BBa_K2535009. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won't be assembled in the wrong place. K2535008
spacerYf This is a 10 nucleotide spacer used to build BBa_K2535013. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won't be assembled in the wrong place. K2535011
spacerYr This is a 10 nucleotide spacer used to build BBa_K2535013. When using Gibson and Golden gate assembly, the spacer at the beginning of the sequence helps create a unique sequence so that if multiple parts have the same beginning sequence, they won't be assembled in the wrong place. K2535012
YdgG YdgG is a membrane transport protein, not native to the Lsr Operon, which exports AI-2 out of the cell (Herzberg et al 2006). BBa_K2535002
pLsr_rbs_LsrR_rbs This part is an AI-2 (autoinducer 2 from the Lsr Operon) dependent promoter that self regulates itself. pLsr is the specific promoter that is bidirectional. Dimers of the LsrR gene bind to and repress this promoter. However, AI-2P (phosphorylated AI-2) can bind to these dimers and cause them to dissociate, depressing the promoter. Since the expression of this plasmid produces LsrR, which represses the promoter pLsr, this system is self regulating. K2535018

References

Gonzalez (2006)
Herzberg et al (2006)