Difference between revisions of "Team:Jilin China/Result/Version 3"

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<h3>Cold-repressible RNA-based thermosensors</h3>
 
<h3>Cold-repressible RNA-based thermosensors</h3>
 
<p>Cold-repressible RNA-based thermosensors are designed based on the RNase III. We constructed their measurement devices and measured the activities at five temperatures: 15, 25, 29, 35 and 37℃. The measured temperature range is lower than the heat-inducible RNA-based thermosensors' temperature range. We also designed a new negtive control, which always has a cleavage site of RNase III, so it will be digest by the enzyme. After measurement, we chose 10 out of 50 thermosensors. </p>
 
<p>Cold-repressible RNA-based thermosensors are designed based on the RNase III. We constructed their measurement devices and measured the activities at five temperatures: 15, 25, 29, 35 and 37℃. The measured temperature range is lower than the heat-inducible RNA-based thermosensors' temperature range. We also designed a new negtive control, which always has a cleavage site of RNase III, so it will be digest by the enzyme. After measurement, we chose 10 out of 50 thermosensors. </p>
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<p>Figure 1 shows the change of activities of different thermosensors. We find that all of these thermosensors' activities increase with temperature increasing. </p>
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<p>Figure 1 shows the change of activities of different thermosensors. We find that all of these thermosensors' activities decrease with temperature decreasing. </p>
 
<img src="https://static.igem.org/mediawiki/2018/4/48/T--Jilin_China--result--R3bar.png" width="95%"></img>
 
<img src="https://static.igem.org/mediawiki/2018/4/48/T--Jilin_China--result--R3bar.png" width="95%"></img>
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<p>For most of the cold-repressible RNA-based thermosensors, the change of activities from 15 to 25℃ is noticeable than 25 to 35℃. And the melting temperature is lower than the heat-inducible RNA thermosensors. Figure 2 shows the fold-change of activity in different temperature. </p>
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<p>For most of the heat-inducible RNA-based thermosensors, the sensing temperature range is between 30 to 40. However, for the cold-repressible RNA-based thermosensors, the sensing temperature range decrease to 15~35℃.</p>
 
<img src="https://static.igem.org/mediawiki/2018/4/48/T--Jilin_China--result--R3bar.png" width="95%"></img>
 
<img src="https://static.igem.org/mediawiki/2018/4/48/T--Jilin_China--result--R3bar.png" width="95%"></img>
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    <p>Discussion: As these results show, cold-repressible RNA-based thermosensors work really well. Their activities decrease with the temperature decreasing. And the sensing temperature range is different from heat-inducible RNA-based thermosensors'. Cold repressible RNA-based thermosensor can sense lower temperature. </p>
 
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     </li>

Revision as of 06:08, 14 October 2018

TOOLKITS
VERSION 3.0


Promoters

VERSION 3.0

  • Results

    We added 10 cold-reprssible RNA-based thermosensors and 8 cold-inducible RNA-based thermosensors to the SynRT Toolkit. After that, we named the updated toolkit as SynRT Toolkit version 3.0.

    Cold-repressible RNA-based thermosensors

    Cold-repressible RNA-based thermosensors are designed based on the RNase III. We constructed their measurement devices and measured the activities at five temperatures: 15, 25, 29, 35 and 37℃. The measured temperature range is lower than the heat-inducible RNA-based thermosensors' temperature range. We also designed a new negtive control, which always has a cleavage site of RNase III, so it will be digest by the enzyme. After measurement, we chose 10 out of 50 thermosensors.

    Figure 1 shows the change of activities of different thermosensors. We find that all of these thermosensors' activities decrease with temperature decreasing.

    For most of the heat-inducible RNA-based thermosensors, the sensing temperature range is between 30 to 40. However, for the cold-repressible RNA-based thermosensors, the sensing temperature range decrease to 15~35℃.

    Discussion: As these results show, cold-repressible RNA-based thermosensors work really well. Their activities decrease with the temperature decreasing. And the sensing temperature range is different from heat-inducible RNA-based thermosensors'. Cold repressible RNA-based thermosensor can sense lower temperature.