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<h2>II. Biofilm formation and culture conditions established</h2>
 
<h2>II. Biofilm formation and culture conditions established</h2>
 
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Revision as of 07:03, 14 October 2018

Notebook of algae-bacteria symbiotic laboratory







Notebook of algae-bacteria symbiotic laboratory



18.05.11
Determine the medium, the growth curve of the algae - to determine the species of the algae, set the alternative species, the initial culture conditions are determined, the focus is on the filamentous green algae (can not be quantified, different traits in different periods, the cultivation is more difficult , want to use non-synthetic biological methods to improve the stability of the film light stress)
18.05.13
got a variety of algae species
The indicator of growth status is determined 1: film thickness (excluded later)
Culture conditions: culture fluid circulation
Digital model: find the most significant amount of the growth rate of algae as the main variable of the post-experiment, the input and output have been formed
Growth indicator 2: dry weight, reactive dyes, enzyme activity
The preliminary plan for the preliminary experiment of 0518 Department of Biosynthesis:
18.05.19
E. coli vs. Pseudomonas putida, E. coli wins
18.05.24
bacteria algae than experimental protocol
18.05.26
Centrifugal separation of algae, let the algae completely separate od Indicator 3 of growth status: chlorophyll
18.06.08
bacteria algae than large experimental data
18.07.04
filtration method, sub-bacterial algae test, failure
18.07.19
proposed two lines - suspension system + preliminary conclusion of suspension system (medium, bacteria and algae ratio) small plastic cup experiment - attempted film extraction
Suspension culture experiments monitored dynamic growth indicators (modeling) used: blood count and chlorophyll
Small experiment of culture dish: improved experiment of film extraction Pre-experiment of blood cell count: standard od and number
18.07.24
The experiment encountered a bottleneck
The literature supports peak bacteria and algae ratio 3:1
Try the signal molecule of the conditional algae exchange - self-sensit
18.07.26
Try a variety of membrane materials and membrane materials than the initial test
18.07.28
Method for establishing membrane shedding rate - shaker, peristaltic pump
18.07.29
screening membrane material combination
18.07.30
making mixer device
18.08.03
mixing device 6 completed
Improvement of growth status indicators: chlorophyll
18.09.08
Growth status indicator: CFU method for measuring algae Culture system BG11 improved-BG11
18.09.13
C. algae CFU experiment began to do
18.09.22
Summary: Comparison of the various methods of counting
18.09.23
Dual Wavelength Spectrophotometry
Algae gradient full-band scanning
18.10.07
Using existing standard systems to verify the effect of parts on membrane stability



Part of our Raw data

I.Suspension co-culture conditions

1. culture medium of co-culture

alage:

0.232

0.321

0.386

0.445

0.411

0.405

0.4

0.264

0.376

0.419

0.434

0.441

0.413

0.421

0.201

0.269

0.34

0.342

0.336

0.282

0.3

0.129

0.103

0.082

0.071

0.079

0.069

0.078

0.095

0.063

0.067

0.078

0.04

0.082

0.063

0.132

0.173

0.116

0.155

0.142

0.168

0.158

0.143

0.141

0.112

0.114

0.097

0.099

0.097

0.118

0.11

0.114

0.118

0.056

0.071

0.061



0.253

0.342

0.407

0.466

0.432

0.426

0.421

0.285

0.397

0.44

0.455

0.462

0.434

0.442

0.222

0.29

0.361

0.363

0.357

0.303

0.321

0.15

0.124

0.103

0.092

0.1

0.09

0.099

0.116

0.084

0.088

0.099

0.061

0.103

0.084

0.156

0.197

0.14

0.179

0.166

0.192

0.182

0.167

0.165

0.136

0.138

0.121

0.123

0.121

0.142

0.134

0.138

0.142

0.08

0.095

0.085

1

2

3

4

5

6

7

9:1

1.265

1.71

2.035

2.33

2.16

2.13

2.105

7:1

1.425

1.985

2.2

2.275

2.31

2.17

2.21

4:1

1.11

1.45

1.805

1.815

1.785

1.515

1.605

2:1

0.75

0.62

0.515

0.46

0.5

0.45

0.495

1:2

0.58

0.42

0.44

0.495

0.305

0.515

0.42

1:4

0.39

0.4925

0.35

0.4475

0.415

0.48

0.455

1:7

0.4175

0.4125

0.34

0.345

0.3025

0.3075

0.3025

1:9

0.355

0.335

0.345

0.355

0.2

0.2375

0.2125




bacteria

0.311

0.419

0.538

0.567

0.511

0.422

0.361

0.357

0.484

0.577

0.538

0.53

0.483

0.421

0.27

0.34

0.414

0.39

0.371

0.284

0.3

0.164

0.104

0.063

0.061

0.003

0.011

0.004

0.113

0.082

0.061

0.017

0.022

0.034

0.024

0.169

0.2

0.1

0.128

0.083

0.099

0.087

0.182

0.159

0.092

0.078

0.032

0.031

0.034

0.149

0.124

0.094

0.097

-0.012

0.003

0.01


0.384

0.492

0.611

0.64

0.584

0.495

0.434

0.43

0.557

0.65

0.611

0.603

0.556

0.494

0.343

0.413

0.487

0.463

0.444

0.357

0.373

0.237

0.177

0.136

0.134

0.076

0.084

0.077

0.186

0.155

0.134

0.09

0.095

0.107

0.097

0.244

0.275

0.175

0.203

0.158

0.174

0.162

0.257

0.234

0.167

0.153

0.107

0.106

0.109

0.224

0.199

0.169

0.172

0.063

0.078

0.085

1

2

3

4

5

6

7

9:1

1.92

2.46

3.055

3.2

2.92

2.475

2.17

7:1

2.15

2.785

3.25

3.055

3.015

2.78

2.47

4:1

1.715

2.065

2.435

2.315

2.22

1.785

1.865

2:1

1.185

0.885

0.68

0.67

0.38

0.42

0.385

1:2

0.93

0.775

0.67

0.45

0.475

0.535

0.485

1:4

0.61

0.6875

0.4375

0.5075

0.395

0.435

0.405

1:7

0.6425

0.585

0.4175

0.3825

0.2675

0.265

0.2725

1:9

0.56

0.4975

0.4225

0.43

0.1575

0.195

0.2125

2. Determine the optimal ratio of bacteria to algae

Initial value

OD:  bacteria:algae

Three parallel
Per groups

 

 

group

proportion

0:1

1:100

1:10

1:1

Actual absorbance

00:00.2

0.002:0.2

0.02 : 0.2

0.2 : 0.2

time/h

group 1

group 2

group 3

group 4

17.5

0

0.006

0.162

0.426

41

0.03

0.018

0.174

0.444

67

0.036

0.108

0.204

0.336

86

0.15

0.216

0.3

0.612

114.5

0.126

0.186

0.42

0.624

160

0.096

0.138

0.21

0.432

186.5

0.174

0.156

0.204

0.348

210.5

0.264

0.222

0.216

0.258

234.5

0.216

0.18

0.258

0.216




time/h

1;1

1;2

1;3

2;1

2;2

2;3

3;1

3;2

3;3

4;1

4;2

4;3

17.5

-0.03

-0.012

0.054

0.006

0.018

-0.006

0.132

0.222

0.132

0.456

0.408

0.42

41

0.012

0.03

0.054

0.012

0.024

0.03

0.186

0.192

0.156

0.462

0.36

0.51

67

0.012

0.018

0.084

0.072

0.126

0.126

0.132

0.15

0.15

0.312

0.318

0.39

86

0.192

0.132

0.126

0.252

0.216

0.186

0.252

0.306

0.342

0.6

0.678

0.558

114.5

0.126

0.12

0.138

0.15

0.204

0.216

0.432

0.42

0.408

0.684

0.564

0.63

160

0.138

0.126

0.024

0.126

0.12

0.168

0.198

0.204

0.234

0.42

0.438

0.444

186.5

0.198

0.192

0.144

0.198

0.144

0.132

0.18

0.228

-0.024

0.24

0.456

0.354

210.5

0.264

0.258

0.276

0.198

0.27

0.198

0.162

0.264

-0.024

0.258

0.24

0.276

234.5

0.204

0.228

0.222

0.144

0.228

0.168

0.246

0.27

-0.012

0.294

0.222

0.138


time/h

group 1

group 2

group 3

group 4

17.5

0.506

0.332

0.56

0.674

41

0.762

0.766

0.87

1.124

67

1.138

1.112

1.216

1.476

86

1.13

1.094

1.262

1.474

114.5

1.264

1.282

1.404

1.662

160

1.18

1.232

1.406

1.766

186.5

1.368667

1.401333

1.646

2.114

210.5

1.272

1.298667

1.588

1.890667

234.5

1.26

1.267333

1.56

2.126667

time/h

1;1

2;1

3;1

4;1

1;2

2;2

3;2

4;2

1;3

2;3

3;3

4;3

17.5

0.241

0.129

0.247

0.377

0.243

0.202

0.317

0.374

0.276

0.168

0.276

0.261

41

0.383

0.389

0.439

0.574

0.365

0.372

0.434

0.566

0.395

0.389

0.433

0.547

67

0.579

0.525

0.649

0.766

0.572

0.549

0.61

0.71

0.558

0.596

0.565

0.74

86

0.56

0.526

0.652

0.749

0.579

0.526

0.643

0.711

0.556

0.59

0.6

0.753

114.5

0.6

0.611

0.738

0.836

0.656

0.642

0.7

0.806

0.642

0.67

0.668

0.851

160

0.643

0.64

0.756

0.91

0.548

0.652

0.722

0.901

0.581

0.556

0.631

0.839

186.5

0.784

0.665

0.875

1.184

0.649

0.676

0.771

0.999

0.62

0.761

0.393

0.988

210.5

0.699

0.636

0.855

0.969

0.599

0.656

0.733

0.932

0.61

0.656

0.4

0.935

234.5

0.682

0.612

0.83

0.993

0.525

0.643

0.73

0.953

0.683

0.646

0.426

1.244

II. Biofilm formation and culture conditions established



Number

materials

Diameter of pores/μm

ration

stability

1

Mixed fiber membrane + blotting paper

0.45

10:1(3.5x10^7)

×

2

Mixed film + toilet paper

0.45

10:1

×

3

Polypropylene film + absorbent paper

0.1

10:1

×

4

Absorbent paper

/

10:1

×

5

Writing paper

/

10:1

×

6

Mixed film + writing paper

0.45

10:1

×

7

Polypropylene film + writing paper

0.1

10:1

×

8

Mixed fiber membrane

0.45

10:1

×

9

Mixed fiber membrane

0.22

10:1

×

10

Mixed fiber membrane

3

10:1

×

11

Mixed fiber membrane

0.45

20:2

×

12

Mixed fiber membrane

3

20:2

×

13

Mixed fiber membrane

3

10:2

×

14

Mixed fiber membrane

0.22

5:1

15

Mixed fiber membrane

0.45

5:1

16

Mixed fiber membrane

0.22

3:1

17

Mixed fiber membrane

0.45

3:1

18

Mixed film (front)

3

3:1

×

19

Qualitative filter paper

/

10:1

20

Qualitative filter paper

/

20:2

21

Qualitative filter paper

/

5:1

22

Qualitative filter paper

/

3:1

23

cotton

/

10:1

24

Mixed film (reverse)

3

3:1

×

25

Cloth

/

10:1

26

Cloth

/

5:1

27

Cloth

/

20;2

28

Cloth

/

3:1

29

Mixed fiber membrane

3

10:1

×

30

Mixed fiber membrane

3

5:1

×

31

Mixed fiber membrane

3

3:1

×

32

Mixed fiber membrane

3

20:2

33

Mixed fiber membrane

3

20:1

×

34

Mixed fiber membrane

3

50:1

35

Mixed fiber membrane

3

100:1

×

36

Mixed fiber membrane

0.45

10:1

37

Mixed fiber membrane

0.45

20:1

×

38

Mixed fiber membrane

0.45

50:1

×

39

Mixed fiber membrane

0.45

100:1

×

40

Mixed fiber membrane

0.45

5:1

×

41

Mixed fiber membrane

0.22

10:1

×

42

Mixed fiber membrane

0.22

20:1

×

43

Mixed fiber membrane

0.22

5:1

×

44

Mixed fiber membrane

0.22

3:1

×

45

Mixed fiber membrane

3

50:1

×

46

Mixed fiber membrane

0.22

10:2

×

47

Mixed film (front)

3

10:4

48

Mixed film (reverse)

3

10:4




III. Establishment of standard method to evaluate the stability of the biofilm




Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

1-1A

1.23

0.793

1.622

1.508

1.08

1.922

20.99186

1-1

0.270319

1-1B

0.233

0.2

0.423

0.511

0.487

0.723

7.77669

1-2A

0.867

0.55

1.151

1.145

0.837

1.451

15.81377

1-2

0.314562

1-2B

0.272

0.189

0.378

0.55

0.476

0.678

7.25726

1-3A

0.72

0.46

0.987

0.998

0.747

1.287

14.02073

1-3

0.466449

1-3B

0.609

0.395

0.829

0.887

0.682

1.129

12.25741

1-4A

0.659

0.437

0.921

0.937

0.724

1.221

13.27893

1-4

0.57297

1-4B

1.073

0.662

1.336

1.351

0.949

1.636

17.81706

1-5A

0.813

0.574

1.2

1.091

0.861

1.5

16.36178

1-5

0.660063

1-5B

0.846

0.551

1.157

2.248

1.676

2.914

31.77002

1-6A

0.955

0.645

1.377

1.233

0.932

1.677

18.33853

1-6

0.547671

1-6B

0.546

0.356

0.726

1.648

1.286

2.052

22.20392


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

1-1A

0.398

0.362

0.756

0.676

0.649

1.056

11.46006

1-1

0.624916

1-1B

0.92

0.68

1.445

1.198

0.967

1.745

19.09323

1-2A

0.532

0.39

0.858

0.81

0.677

1.158

12.61492

1-2

0.538813

1-2B

0.71

0.464

1.048

0.988

0.751

1.348

14.73822

1-3A

0.255

0.232

0.492

0.533

0.519

0.792

8.5433

1-3

0.669452

1-3B

0.919

0.578

1.276

0.512

0.865

1.576

17.30254

1-4A

0.234

0.177

0.38

1.141

0.464

0.68

7.25216

1-4

0.698348

1-4B

0.863

0.549

1.229

0.524

0.836

1.529

16.78929

1-5A

0.246

0.206

0.434

0.984

0.493

0.734

7.85996

1-5

0.643324

1-5B

0.706

0.445

0.995

0.522

0.732

1.295

14.17671

1-6A

0.244

0.228

0.458

1.045

0.515

0.758

8.1056

1-6

0.656481

1-6B

0.767

0.482

1.109

0.278

0.769

1.409

15.49015


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

2-1A

1.232

0.843

1.703

1.51

1.13

2.003

21.87455

2-1

0.207334

2-1B

0.168

0.122

0.239

0.446

0.409

0.539

5.72161

2-2A

1.176

0.811

1.604

1.454

1.098

1.904

20.75516

2-2

0.309094

2-2B

0.405

0.281

0.562

0.683

0.568

0.862

9.28534

2-3A

0.903

0.657

1.32

1.181

0.944

1.62

17.64876

2-3

0.428306

2-3B

0.66

0.443

0.917

0.938

0.73

1.217

13.22221

2-4A

0.905

0.62

1.228

1.183

0.907

1.528

16.61538

2-4

0.441088

2-4B

0.665

0.46

0.91

0.943

0.747

1.21

13.11268

2-5A

0.987

0.656

1.328

1.265

0.943

1.628

17.73838

2-5

0.381236

2-5B

0.478

0.341

0.709

0.756

0.628

1.009

10.92905

2-6A

1.308

0.874

1.697

1.586

1.161

1.997

21.74963

2-6

0.36848

2-6B

0.679

0.441

0.872

0.957

0.728

1.172

12.69052


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

2-1A

0.564

0.429

0.877

0.842

0.716

1.177

12.77745

2-1

0.596511

2-1B

0.981

0.678

1.428

1.259

0.965

1.728

18.88998

2-2A

0.61

0.505

1.062

0.888

0.792

1.362

14.84898

2-2

0.52318

2-2B

0.89

0.561

1.193

1.168

0.848

1.493

16.29269

2-3A

0.414

0.3

0.635

0.692

0.587

0.935

10.12041

2-3

0.645336

2-3B

1

0.647

1.384

1.278

0.934

1.684

18.4148

2-4A

0.305

0.222

0.47

0.583

0.509

0.77

8.294

2-4

0.710082

2-4B

1.152

0.737

1.557

1.43

1.024

1.857

20.31409

2-5A

0.524

0.451

0.93

0.802

0.738

1.23

13.37482

2-5

0.627275

2-5B

1.34

0.864

1.76

1.618

1.151

2.06

22.50902

2-6A

0.429

0.38

0.77

0.707

0.667

1.07

11.59576

2-6

0.639574

2-6B

1.517

0.763

1.585

1.795

1.05

1.885

20.57665


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

3-1A

1.786

1.18

2.119

2.064

1.467

2.419

26.24085

3-1

0.205789

3-1B

6.275

0.213

0.383

6.553

0.5

0.683

6.79931

3-2A

1.521

0.967

1.894

1.799

1.254

2.194

23.92382

3-2

0.210971

3-2B

0.212

0.143

0.299

0.49

0.43

0.599

6.39675

3-3A

1.482

0.95

1.872

1.76

1.237

2.172

23.69242

3-3

0.240367

3-3B

0.291

0.194

0.399

0.569

0.481

0.699

7.49689

3-4A

1.632

1.049

1.982

1.91

1.336

2.282

24.83146

3-4

0.259145

3-4B

0.374

0.241

0.506

0.652

0.528

0.806

8.68582

3-5A

1.578

1.011

1.979

1.856

1.298

2.279

24.85875

3-5

0.394575

3-5B

0.666

0.532

1.18

0.944

0.819

1.48

16.20122

3-6A

1.145

0.897

1.71

1.423

1.184

2.01

21.8813

3-6

0.324573

3-6B

0.495

0.332

0.673

0.773

0.619

0.973

10.51495


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

3-1A

0.885

0.548

1.109

1.163

0.835

1.409

15.31771

3-1

0.394527

3-1B

0.496

0.317

0.626

0.774

0.604

0.926

9.98102

3-2A

0.82

0.51

1.036

1.098

0.797

1.336

14.51638

3-2

0.418606

3-2B

0.503

0.311

0.665

0.781

0.598

0.965

10.45185

3-3A

0.882

0.548

1.146

1.16

0.835

1.446

15.7564

3-3

0.333124

3-3B

0.313

0.204

0.432

0.591

0.491

0.732

7.87078

3-4A

0.824

0.513

1.094

1.102

0.8

1.394

15.19874

3-4

0.456218

3-4B

0.631

0.404

0.872

0.909

0.691

1.172

12.75134

3-5A

0.827

0.533

1.101

1.105

0.82

1.401

15.25065

3-5

0.445687

3-5B

0.601

0.377

0.827

0.879

0.664

1.127

12.26207

3-6A

0.774

0.482

1.038

1.052

0.769

1.338

14.58688

3-6

0.405307

3-6B

0.432

0.269

0.616

0.71

0.556

0.916

9.94156


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

1-1A

1.838

1.334

1.878

2.116

1.621

2.178

23.14368

1-1

0.152838

1-1B

-0.002

-0.104

0.078

0.276

0.183

0.378

4.1754

1-2A

1.959

1.787

1.897

2.237

2.074

2.197

22.66153

1-2

0.15882

1-2B

0.002

-0.102

0.087

0.28

0.185

0.387

4.27865

1-3A

1.96

1.833

1.885

2.238

2.12

2.185

22.44841

1-3

0.182859

1-3B

0.061

-0.063

0.168

2.217

0.224

0.468

5.02348

1-4A

1.939

1.676

1.883

0.225

1.963

2.183

22.82753

1-4

0.130565

1-4B

-0.053

-0.128

0.025

2.229

0.159

0.325

3.42807

1-5A

1.951

1.879

1.831

0.425

2.166

2.131

21.88271

1-5

0.223914

1-5B

0.147

0

0.282

1.765

0.287

0.582

6.31352

1-6A

1.487

0.904

1.686

0.167

1.191

1.986

21.6866

1-6

0.104997

1-6B

-0.111

-0.176

-0.069

0.278

0.111

0.231

2.54417


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

2-1A

1.476

1.2

1.853

1.754

1.487

2.153

23.08275

2-1

0.262895

2-1B

0.256

0.072

0.445

0.534

0.359

0.745

8.23267

2-2A

1.785

1.276

1.831

2.063

1.563

2.131

22.68029

2-2

0.472915

2-2B

1.427

0.846

1.576

1.705

1.133

1.876

20.34938

2-3A

0.278

0.287

0.3

3.09078

2-3

2-3B

0.278

0.287

0.3

3.09078

2-4A

1.87

1.501

1.812

2.148

1.788

2.112

22.10184

2-4

0.352981

2-4B

0.559

0.258

0.794

0.837

0.545

1.094

12.05764

2-5A

1.856

1.524

1.765

2.134

1.811

2.065

21.51059

2-5

0.451895

2-5B

1.058

0.57

1.317

1.336

0.857

1.617

17.73479

2-6A

1.707

1.136

1.78

1.985

1.423

2.08

22.29778

2-6

0.390238

2-6B

0.778

0.395

1

1.056

0.682

1.3

14.27024


Correction value

group number

λ666

λ653

λ470

λ666

λ653

λ470

c

Shedding rate

3-1A

1.832

1.315

1.872

2.11

1.602

2.172

23.10232

3-1

0.256887

3-1B

0.23

0.041

0.42

0.508

0.328

0.72

7.98624

3-2A

1.891

1.496

1.84

2.169

1.783

2.14

22.43966

3-2

0.283524

3-2B

0.294

0.073

0.5

0.572

0.36

0.8

8.87984

3-3A

1.845

1.372

1.832

2.123

1.659

2.132

22.5395

3-3

0.340541

3-3B

0.484

0.195

0.75

0.762

0.482

1.05

11.63926

3-4A

1.734

1.155

1.837

2.012

1.442

2.137

22.94181

3-4

0.340739

3-4B

0.507

0.206

0.77

0.785

0.493

1.07

11.85748

3-5A

1.577

0.993

1.726

1.855

1.28

2.026

21.8885

3-5

0.386312

3-5B

0.63

0.291

0.944

0.908

0.578

1.244

13.77864

3-6A

1.823

1.288

1.87

2.101

1.575

2.17

23.12092

3-6

0.263178

3-6B

0.249

0.048

0.444

0.527

0.335

0.744

8.25834




Name Count Shedding rate Name Count Shedding rate Name Count Shedding rate
1-1-A1.b 587 0.324511 1 1 A1.bmp 363 0.305927 Name Count
1-1-A2.b 486 0.370466 2 1 A1.bmp 493 0.295714 1 1 A1.bmp 0.65362 177
1-2-A1.b 107 0.548523 3 1 A1.bmp 270 0.429175 1 1 A1.bmp 0.401389 431 1 1 A2.bmp 0.558577 211
1-2-A2.b 191 0.39557 1 1 A2.bmp 361 0.188764 2 1 A1.bmp 0.566441 385 2 1 A1.bmp 0.65917 197
1-3-A1.b 445 0.589104 2 1 A2.bmp 376 0.304991 3 1 A1.bmp 0.437247 278 2 1 A2.bmp 0.590361 204
1-3-A2.b 536 0.557391 3 1 A2.bmp 310 0.376258 1 1 A2.bmp 0.43909 419 3 1 A1.bmp 0.472934 185
1-4-A1.b 264 0.649867 1 1 B1.bmp 160 2 1 A2.bmp 0.61215 332 3 1 A2.bmp 0.467085 170
1-4-A2.b 203 0.713681 2 1 B1.bmp 207 3 1 A2.bmp 0.389277 262 1 2 A1.bmp 0.632495 190
1-5-A1.b 224 0.68 3 1 B1.bmp 203 1 2 A1.bmp 0.514739 214 1 2 A2.bmp 0.618304 171
1-5-A2.b 206 0.725699 1 1 B2.bmp 84 2 2 A1.bmp 0.633867 320 2 2 A1.bmp 0.613095 195
1-6-A1.b 260 0.651475 2 1 B2.bmp 165 3 2 A1.bmp 0.697616 241 2 2 A2.bmp 0.583541 167
1-6-A2.b 305 0.621588 3 1 B2.bmp 187 1 2 A2.bmp 0.570796 194 3 2 A1.bmp 0.763209 121
2-1-A1.b 413 0.258528 1 2 A1.bmp 392 0.395062 2 2 A2.bmp 0.652387 284 3 2 A2.bmp 0.670082 161
2-1-A2.b 353 0.206742 2 2 A1.bmp 377 0.486376 3 2 A2.bmp 0.65 287 1 3 A1.bmp 0.649798 173
2-2-A1.b 409 0.30678 3 2 A1.bmp 330 0.495413 1 3 A1.bmp 0.469914 370 1 3 A2.bmp 0.571956 232
2-2-A2.b 440 0.376771 1 2 A2.bmp 339 0.447883 2 3 A1.bmp 0.520286 201 2 3 A1.bmp 0.717626 157
2-3-A1.b 905 0.349856 2 2 A2.bmp 247 0.606688 3 3 A1.bmp 0.541374 327 2 3 A2.bmp 0.736287 125
2-3-A2.b 974 0.284875 3 2 A2.bmp 325 0.497682 1 3 A2.bmp 0.428148 386 3 3 A1.bmp 0.518519 182
2-4-A1.b 293 0.691254 1 2 B1.bmp 256 2 3 A2.bmp 0.485294 210 3 3 A2.bmp 0.693252 150
2-4-A2.b 231 0.724344 2 2 B1.bmp 357 3 3 A2.bmp 0.542484 350 1 4 A1.bmp 0.517007 284
2-5-A1.b 321 0.682178 3 2 B1.bmp 324 1 4 A1.bmp 0.497436 294 1 4 A2.bmp 0.473934 222
2-5-A2.b 247 0.723094 1 2 B2.bmp 275 2 4 A1.bmp 0.387608 425 2 4 A1.bmp 0.679389 168
2-6-A1.b 332 0.736926 2 2 B2.bmp 381 3 4 A1.bmp 0.614545 318 2 4 A2.bmp 0.686441 148
2-6-A2.b 320 0.772566 3 2 B2.bmp 322 1 4 A2.bmp 0.516611 291 3 4 A1.bmp 0.465328 293
3-1-A1.b 687 0.375455 1 3 A1.bmp 290 0.451796 2 4 A2.bmp 0.446959 391 3 4 A2.bmp 0.559415 241
3-1-A2.b 713 0.360538 2 3 A1.bmp 300 0.563319 3 4 A2.bmp 0.595062 328 1 5 A1.bmp 0.723539 175
3-2-A1.b 435 0.358407 3 3 A1.bmp 158 0.649667 1 5 A1.bmp 0.396936 433 1 5 A2.bmp 0.552083 215
3-2-A2.b 453 0.329882 1 3 A2.bmp 302 0.419231 2 5 A1.bmp 0.408618 398 2 5 A1.bmp 0.65897 192
3-3-A1.b 396 0.644843 2 3 A2.bmp 206 0.615672 3 5 A1.bmp 0.501269 393 2 5 A2.bmp 0.686992 154
3-3-A2.b 415 0.632743 3 3 A2.bmp 182 0.712934 1 5 A2.bmp 0.418429 385 3 5 A1.bmp 0.714286 164
3-4-A1.b 439 0.624786 1 3 B1.bmp 239 2 5 A2.bmp 0.485755 361 3 5 A2.bmp 0.697588 163
3-4-A2.b 348 0.657817 2 3 B1.bmp 387 3 5 A2.bmp 0.529412 336 1 6 A1.bmp 0.434091 249
3-5-A1.b 325 0.773519 3 3 B1.bmp 293 1 6 A1.bmp 0.570565 213 1 6 A2.bmp 0.521411 190
3-5-A2.b 336 0.753485 1 3 B2.bmp 218 2 6 A1.bmp 0.536716 347 2 6 A1.bmp 0.565121 197
3-6-A1.b 319 0.708676 2 3 B2.bmp 330 3 6 A1.bmp 0.520301 319 2 6 A2.bmp 0.452174 252
3-6-A2.b 364 0.71316 3 3 B2.bmp 452 1 6 A2.bmp 0.547655 299 3 6 A1.bmp 0.64775 180
1 4 A1.bmp 144 0.518395 2 6 A2.bmp 0.553398 276 3 6 A2.bmp 0.734317 144
1-1-B1.b 282 2 4 A1.bmp 320 0.580603 3 6 A2.bmp 0.471891 310
1-1-B2.b 286 3 4 A1.bmp 163 0.757079 1 1 B1.bmp 289
1-2-B1.b 130 1 4 A2.bmp 149 0.404 1 2 B1.bmp 227 4 1 A1.bmp 0.643369 199
1-2-B2.b 125 2 4 A2.bmp 216 0.594747 1 3 B1.bmp 328 4 1 A2.bmp 0.509158 268
1-3-B1.b 638 3 4 A2.bmp 158 0.692607 1 4 B1.bmp 291 5 1 A1.bmp 0.696774 235
1-3-B2.b 675 1 4 B1.bmp 155 1 5 B1.bmp 285 5 1 A2.bmp 0.604588 293
1-4-B1.b 490 2 4 B1.bmp 443 1 6 B1.bmp 283 6 1 A1.bmp 0.641068 215
1-4-B2.b 506 3 4 B1.bmp 508 2 1 B1.bmp 503 6 1 A2.bmp 0.638264 225
1-5-B1.b 476 1 4 B2.bmp 101 2 2 B1.bmp 554 4 2 A1.bmp 0.749169 151
1-5-B2.b 545 2 4 B2.bmp 317 2 3 B1.bmp 218 4 2 A2.bmp 0.849421 78
1-6-B1.b 486 3 4 B2.bmp 356 2 4 B1.bmp 269 5 2 A1.bmp 0.741214 162
1-6-B2.b 501 1 5 A1.bmp 270 0.300518 2 5 B1.bmp 275 5 2 A2.bmp 0.751984 125
2-1-B1.b 144 2 5 A1.bmp 218 0.581574 2 6 B1.bmp 402 6 2 A1.bmp 0.772616 186
2-1-B2.b 92 3 5 A1.bmp 208 0.665056 3 1 B1.bmp 216 6 2 A2.bmp 0.801757 158
2-2-B1.b 181 1 5 A2.bmp 216 0.345455 3 2 B1.bmp 556 4 3 A1.bmp 0.692057 283
2-2-B2.b 266 2 5 A2.bmp 170 0.719472 3 3 B1.bmp 386 4 3 A2.bmp 0.639286 303
2-3-B1.b 487 3 5 A2.bmp 236 0.641337 3 4 B1.bmp 507 5 3 A1.bmp 0.809079 143
2-3-B2.b 388 1 5 B1.bmp 116 3 5 B1.bmp 395 5 3 A2.bmp 0.814768 148
2-4-B1.b 656 2 5 B1.bmp 303 3 6 B1.bmp 346 6 3 A1.bmp 0.663717 190
2-4-B2.b 607 3 5 B1.bmp 413 1 1 B2.bmp 328 6 3 A2.bmp 0.748239 143
2-5-B1.b 689 1 5 B2.bmp 114 1 2 B2.bmp 258 4 4 A1.bmp 0.744624 190
2-5-B2.b 645 2 5 B2.bmp 436 1 3 B2.bmp 289 4 4 A2.bmp 0.70936 177
2-6-B1.b 930 3 5 B2.bmp 422 1 4 B2.bmp 311 5 4 A1.bmp 0.72103 195
2-6-B2.b 1087 1 6 A1.bmp 129 0.724359 1 5 B2.bmp 277 5 4 A2.bmp 0.814229 141
3-1-B1.b 413 2 6 A1.bmp 157 0.761035 1 6 B2.bmp 362 6 4 A1.bmp 0.808889 129
3-1-B2.b 402 3 6 A1.bmp 101 0.827055 2 1 B2.bmp 524 6 4 A2.bmp 0.8448 97
3-2-B1.b 243 1 6 A2.bmp 125 0.734607 2 2 B2.bmp 533 4 5 A1.bmp 0.755072 169
3-2-B2.b 223 2 6 A2.bmp 137 0.777597 2 3 B2.bmp 198 4 5 A2.bmp 0.809295 119
3-3-B1.b 719 3 6 A2.bmp 73 0.870567 2 4 B2.bmp 316 5 5 A1.bmp 0.864516 105
3-3-B2.b 715 1 6 B1.bmp 339 2 5 B2.bmp 341 5 5 A2.bmp 0.809976 160
3-4-B1.b 731 2 6 B1.bmp 500 2 6 B2.bmp 342 6 5 A1.bmp 0.70232 231
3-4-B2.b 669 3 6 B1.bmp 483 3 1 B2.bmp 167 6 5 A2.bmp 0.681885 216
3-5-B1.b 1110 1 6 B2.bmp 346 3 2 B2.bmp 533 4 6 A1.bmp 0.693452 206
3-5-B2.b 1027 2 6 B2.bmp 479 3 3 B2.bmp 415 4 6 A2.bmp 0.753205 154
3-6-B1.b 776 3 6 B2.bmp 491 3 4 B2.bmp 482 5 6 A1.bmp 0.736342 222
3-6-B2.b 905 3 5 B2.bmp 378 5 6 A2.bmp 0.753482 177
3 6 B2.bmp 277 6 6 A1.bmp 0.720772 246
4-1-A1.b 380 0.37397 4 1 A1.bmp 400 0.28952 6 6 A2.bmp 0.726716 223
4-1-A2.b 341 0.373162 5 1 A1.bmp 357 0.283133 4 1 A1.bmp 0.6102 214
4-2-A1.b 747 0.348168 6 1 A1.bmp 704 0.391003 5 1 A1.bmp 0.528724 484 7 1 A1.bmp 0.636147 306
4-2-A2.b 818 0.274823 4 1 A2.bmp 513 0.236607 6 1 A1.bmp 0.481888 472 7 1 A2.bmp 0.549869 343
4-3-A1.b 505 0.491952 5 1 A2.bmp 400 0.233716 4 1 A2.bmp 0.647834 187 8 1 A1.bmp 0.625 261
4-3-A2.b 562 0.515517 6 1 A2.bmp 784 0.33503 5 1 A2.bmp 0.589547 534 8 1 A2.bmp 0.625551 255
4-4-A1.b 348 0.678967 4 2 A1.bmp 318 0.485437 6 1 A2.bmp 0.449 551 9 1 A1.bmp 0.364879 604
4-4-A2.b 361 0.666359 5 2 A1.bmp 346 0.425249 4 2 A1.bmp 0.674129 262 9 1 A2.bmp 0.643527 190
4-5-A1.b 320 0.692308 6 2 A1.bmp 841 0.446346 5 2 A1.bmp 0.515643 418 7 2 A1.bmp 0.675481 270
4-5-A2.b 203 0.758907 4 2 A2.bmp 347 0.445687 6 2 A1.bmp 0.597403 403 7 2 A2.bmp 0.638849 251
4-6-A1.b 243 0.79213 5 2 A2.bmp 362 0.431711 4 2 A2.bmp 0.686003 249 8 2 A1.bmp 0.526839 238
4-6-A2.b 272 0.697778 6 2 A2.bmp 680 0.511494 5 2 A2.bmp 0.669903 374 8 2 A2.bmp 0.412892 337
5-1-A1.b 644 0.445306 4 3 A1.bmp 157 0.705441 6 2 A2.bmp 0.652559 353 9 2 A1.bmp 0.664748 233
5-1-A2.b 651 0.468137 5 3 A1.bmp 190 0.595745 4 3 A1.bmp 0.707293 293 9 2 A2.bmp 0.569849 428
5-2-A1.b 506 0.546188 6 3 A1.bmp 502 0.658736 5 3 A1.bmp 0.633958 332 7 3 A1.bmp 0.711388 185
5-2-A2.b 367 0.604526 4 3 A2.bmp 224 0.645008 6 3 A1.bmp 0.601093 292 7 3 A2.bmp 0.705167 194
5-3-A1.b 411 0.585685 5 3 A2.bmp 239 0.518145 4 3 A2.bmp 0.636542 370 8 3 A1.bmp 0.65764 177
5-3-A2.b 307 0.578297 6 3 A2.bmp 589 0.637984 5 3 A2.bmp 0.65874 287 8 3 A2.bmp 0.639229 206
5-4-A1.b 336 0.745455 4 4 A1.bmp 297 0.566423 6 3 A2.bmp 0.680492 208 9 3 A1.bmp 0.591727 227
5-4-A2.b 259 0.755891 5 4 A1.bmp 165 0.708481 4 4 A1.bmp 0.608737 412 9 3 A2.bmp 0.608392 224
5-5-A1.b 123 0.855803 6 4 A1.bmp 645 0.547686 5 4 A1.bmp 0.668874 350 7 4 A1.bmp 0.671756 258
5-5-A2.b 115 0.879581 4 4 A2.bmp 271 0.577223 6 4 A1.bmp 0 317 7 4 A2.bmp 0.62379 272
5-6-A1.b 216 0.823385 5 4 A2.bmp 200 0.646018 4 4 A2.bmp 0.583728 440 8 4 A1.bmp 0.637097 180
5-6-A2.b 235 0.78733 6 4 A2.bmp 513 0.645718 5 4 A2.bmp 0.603235 417 8 4 A2.bmp 0.550403 223
6-1-A1.b 406 0.518957 4 5 A1.bmp 287 0.612162 6 4 A2.bmp 0 258 9 4 A1.bmp 0.261084 300
6-1-A2.b 323 0.537911 5 5 A1.bmp 298 0.535826 4 5 A1.bmp 0.671789 299 9 4 A2.bmp 0.321348 302
6-2-A1.b 373 0.604873 6 5 A1.bmp 515 0.654362 5 5 A1.bmp 0.697382 289 7 5 A1.bmp 0.555556 324
6-2-A2.b 306 0.600522 4 5 A2.bmp 266 0.5744 6 5 A1.bmp 0.682731 237 7 5 A2.bmp 0.406732 423
6-3-A1.b 303 0.494157 5 5 A2.bmp 255 0.558059 4 5 A2.bmp 0.685649 276 8 5 A1.bmp 0.490046 333
6-3-A2.b 236 0.564576 6 5 A2.bmp 552 0.606557 5 5 A2.bmp 0.636364 380 8 5 A2.bmp 0.458054 323
6-4-A1.b 240 0.704433 4 6 A1.bmp 110 0.741176 6 5 A2.bmp 0.635475 261 9 5 A1.bmp 0.516393 295
6-4-A2.b 151 0.743633 5 6 A1.bmp 135 0.800591 4 6 A1.bmp 0.460568 513 9 5 A2.bmp 0.487663 353
6 6 A1.bmp 347 0.737519 5 6 A1.bmp 0.807074 180 7 6 A1.bmp 0.74812 201
4 6 A2.bmp 137 0.676123 6 6 A1.bmp 0.535452 380 7 6 A2.bmp 0.730408 258
4-1-B1.b 227 5 6 A2.bmp 176 0.740413 4 6 A2.bmp 0.416838 568 8 6 A1.bmp 0.495756 297
4-1-B2.b 203 6 6 A2.bmp 347 0.737915 5 6 A2.bmp 0.717494 239 8 6 A2.bmp 0.597744 214
4-2-B1.b 399 4 1 B1.bmp 163 6 6 A2.bmp 0.539638 331 9 6 A1.bmp 0.755039 158
4-2-B2.b 310 4 2 B1.bmp 300 4 1 B1.bmp 335 9 6 A2.bmp 0.777603 141
4-3-B1.b 489 4 3 B1.bmp 376 4 2 B1.bmp 542
4-3-B2.b 598 4 4 B1.bmp 388 4 3 B1.bmp 708
4-4-B1.b 736 4 5 B1.bmp 453 4 4 B1.bmp 641
4-4-B2.b 721 4 6 B1.bmp 315 4 5 B1.bmp 612
4-5-B1.b 720 5 1 B1.bmp 141 4 6 B1.bmp 438
4-5-B2.b 639 5 2 B1.bmp 256 5 1 B1.bmp 543
4-6-B1.b 926 5 3 B1.bmp 280 5 2 B1.bmp 445
4-6-B2.b 628 5 4 B1.bmp 401 5 3 B1.bmp 575
5-1-B1.b 517 5 5 B1.bmp 344 5 4 B1.bmp 707
5-1-B2.b 573 5 6 B1.bmp 542 5 5 B1.bmp 666
5-2-B1.b 609 6 1 B1.bmp 452 5 6 B1.bmp 753
5-2-B2.b 561 6 2 B1.bmp 678 6 1 B1.bmp 439
5-3-B1.b 581 6 3 B1.bmp 969 6 2 B1.bmp 598
5-3-B2.b 421 6 4 B1.bmp 781 6 3 B1.bmp 440
5-4-B1.b 984 6 5 B1.bmp 975 6 4 B1.bmp
5-4-B2.b 802 6 6 B1.bmp 975 6 5 B1.bmp 510
5-5-B1.b 730 4 1 B2.bmp 159 6 6 B1.bmp 438
5-5-B2.b 840 4 2 B2.bmp 279 4 1 B2.bmp 344
5-6-B1.b 1007 4 3 B2.bmp 407 4 2 B2.bmp 544
5-6-B2.b 870 4 4 B2.bmp 370 4 3 B2.bmp 648
6-1-B1.b 438 4 5 B2.bmp 359 4 4 B2.bmp 617
6-1-B2.b 376 4 6 B2.bmp 286 4 5 B2.bmp 602
6-2-B1.b 571 5 1 B2.bmp 122 4 6 B2.bmp 406
6-2-B2.b 460 5 2 B2.bmp 275 5 1 B2.bmp 767
6-3-B1.b 296 5 3 B2.bmp 257 5 2 B2.bmp 759
6-3-B2.b 306 5 4 B2.bmp 365 5 3 B2.bmp 554
6-4-B1.b 572 5 5 B2.bmp 322 5 4 B2.bmp 634
6-4-B2.b 438 5 6 B2.bmp 502 5 5 B2.bmp 665
6 1 B2.bmp 395 5 6 B2.bmp 607
7-1-A1.b 383 0.623772 6 2 B2.bmp 712 6 1 B2.bmp 449
7-1-A2.b 403 0.548206 6 3 B2.bmp 1038 6 2 B2.bmp 663
7-2-A1.b 370 0.65709 6 4 B2.bmp 935 6 3 B2.bmp 443
7-2-A2.b 345 0.629431 6 5 B2.bmp 851 6 4 B2.bmp
7-3-A1.b 411 0.547357 6 6 B2.bmp 977 6 5 B2.bmp 455
7-3-A2.b 428 0.523915 6 6 B2.bmp 388
7-4-A1.b 354 0.681368 7 1 A1.bmp 0.339244
7-4-A2.b 270 0 8 1 A1.bmp 0.392725
7-5-A1.b 274 0.724346 9 1 A1.bmp 0.469333 7 1 A1.bmp 0.387211 345
7-5-A2.b 258 0.75452 7 1 A2.bmp 0.354497 8 1 A1.bmp 0.480808 257
7-6-A1.b 311 0 8 1 A2.bmp 0.482699 9 1 A1.bmp 0.207463 531
7-6-A2.b 244 0 9 1 A2.bmp 0.46817 7 1 A2.bmp 0.296937 528
8-1-A1.b 747 0.297272 7 2 A1.bmp 0.435593 8 1 A2.bmp 0.442043 284
8-1-A2.b 718 0.295388 8 2 A1.bmp 0.470644 9 1 A2.bmp 0.303509 397
8-2-A1.b 460 0.580292 9 2 A1.bmp 0.531943 7 2 A1.bmp 0.432574 324
8-2-A2.b 581 0.545027 7 2 A2.bmp 0.355177 8 2 A1.bmp 0.363636 245
8-3-A1.b 318 0.612195 8 2 A2.bmp 0.410433 9 2 A1.bmp 0.445026 318
8-3-A2.b 70 0.87037 9 2 A2.bmp 0.699593 7 2 A2.bmp 0.419469 328
8-4-A1.b 414 0.653556 7 3 A1.bmp 0.431046 8 2 A2.bmp 0.328704 290
8-4-A2.b 268 0.74717 8 3 A1.bmp 0.486074 9 2 A2.bmp 0.390649 404
8-5-A1.b 242 0.819268 9 3 A1.bmp 0.632469 7 3 A1.bmp 0.485994 367
8-5-A2.b 218 0.809107 7 3 A2.bmp 0.359357 8 3 A1.bmp 0.508982 164
8-6-A1.b 248 0.730727 8 3 A2.bmp 0.441745 9 3 A1.bmp 0.475124 211
8-6-A2.b 203 0.762016 9 3 A2.bmp 0.673988 7 3 A2.bmp 0.3821 359
9-1-A1.b 770 0.338488 7 4 A1.bmp 0.504492 8 3 A2.bmp 0.457143 190
9-1-A2.b 821 0.340562 8 4 A1.bmp 0.567127 9 3 A2.bmp 0.489083 234
9-2-A1.b 470 0.596567 9 4 A1.bmp 0.595455 7 4 A1.bmp 0.435275 349
9-2-A2.b 562 0.544201 7 4 A2.bmp 0.529412 8 4 A1.bmp 0.376206 194
9-3-A1.b 352 0.739452 8 4 A2.bmp 0.636057 9 4 A1.bmp 0.319846 353
9-3-A2.b 338 0.731959 9 4 A2.bmp 0.584027 7 4 A2.bmp 0.311644 402
9-4-A1.b 352 0.671028 7 5 A1.bmp 0.522232 8 4 A2.bmp 0.449086 211
9-4-A2.b 300 0.70902 8 5 A1.bmp 0.614652 9 4 A2.bmp 0.317647 348
9-5-A1.b 361 0.685266 9 5 A1.bmp 0.544304 7 5 A1.bmp 0.442225 391
9-5-A2.b 308 0.713755 7 5 A2.bmp 0.453597 8 5 A1.bmp 0.281818 316
9-6-A1.b 357 0.646184 8 5 A2.bmp 0.527002 9 5 A1.bmp 0.508065 305
9-6-A2.b 299 0.704253 9 5 A2.bmp 0.547794 7 5 A2.bmp 0.359511 367
7 6 A1.bmp 0.53997 8 5 A2.bmp 0.377193 284
7-1-B1.b 635 8 6 A1.bmp 0.541726 9 5 A2.bmp 0.478873 296
7-1-B2.b 489 9 6 A1.bmp 0.599162 7 6 A1.bmp 0.368421 432
7-2-B1.b 709 7 6 A2.bmp 0.502183 8 6 A1.bmp 0.350195 334
7-2-B2.b 586 8 6 A2.bmp 0.546267 9 6 A1.bmp 0.501969 253
7-3-B1.b 497 9 6 A2.bmp 0.591153 7 6 A2.bmp 0.310861 368
7-3-B2.b 471 7 1 B1.bmp 8 6 A2.bmp 0.392079 307
7-4-B1.b 757 7 2 B1.bmp 9 6 A2.bmp 0.476285 265
7 3 B1.bmp 7 1 B1.bmp 218
7-5-B1.b 720 7 4 B1.bmp 7 2 B1.bmp 247
7-5-B2.b 793 7 5 B1.bmp 7 3 B1.bmp 347
7 6 B1.bmp 7 4 B1.bmp 269
8 1 B1.bmp 7 5 B1.bmp 310
8-1-B1.b 316 8 2 B1.bmp 7 6 B1.bmp 252
8-1-B2.b 301 8 3 B1.bmp 8 1 B1.bmp 238
8-2-B1.b 636 8 4 B1.bmp 8 2 B1.bmp 140
8-2-B2.b 696 8 5 B1.bmp 8 3 B1.bmp 170
8-3-B1.b 502 8 6 B1.bmp 8 4 B1.bmp 117
8-3-B2.b 470 9 1 B1.bmp 8 5 B1.bmp 124
8-4-B1.b 781 9 2 B1.bmp 8 6 B1.bmp 180
8-4-B2.b 792 9 3 B1.bmp 9 1 B1.bmp 139
8-5-B1.b 1097 9 4 B1.bmp 9 2 B1.bmp 255
8-5-B2.b 924 9 5 B1.bmp 9 3 B1.bmp 191
8-6-B1.b 673 9 6 B1.bmp 9 4 B1.bmp 166
8-6-B2.b 650 7 1 B2.bmp 9 5 B1.bmp 315
9-1-B1.b 394 7 2 B2.bmp 9 6 B1.bmp 255
9-1-B2.b 424 7 3 B2.bmp 7 1 B2.bmp 223
9-2-B1.b 695 7 4 B2.bmp 7 2 B2.bmp 237
9-2-B2.b 671 7 5 B2.bmp 7 3 B2.bmp 222
9-3-B1.b 999 7 6 B2.bmp 7 4 B2.bmp 182
9-3-B2.b 923 8 1 B2.bmp 7 5 B2.bmp 206
9-4-B1.b 718 8 2 B2.bmp 7 6 B2.bmp 166
9-4-B2.b 731 8 3 B2.bmp 8 1 B2.bmp 225
9-5-B1.b 786 8 4 B2.bmp 8 2 B2.bmp 142
9-5-B2.b 768 8 5 B2.bmp 8 3 B2.bmp 160
9-6-B1.b 652 8 6 B2.bmp 8 4 B2.bmp 172
9-6-B2.b 712 9 1 B2.bmp 8 5 B2.bmp 172
9 2 B2.bmp 8 6 B2.bmp 198
9 3 B2.bmp 9 1 B2.bmp 173
9 4 B2.bmp 9 2 B2.bmp 259
9 5 B2.bmp 9 3 B2.bmp 224
9 6 B2.bmp 9 4 B2.bmp 162
9 5 B2.bmp 272
9 6 B2.bmp 241



IV.Screening materials for biofilm attachment




Name

Count

1

1

A1.bmp

0.415525

128

1

2

A1.bmp

0.175824

150

1

3

A1.bmp

0.23125

123

1

1

A2.bmp

0.431694

104

1

2

A2.bmp

0.242647

103

1

3

A2.bmp

0.368056

91

2

1

A1.bmp

0.195946

119

2

2

A1.bmp

0.260638

139

2

3

A1.bmp

0.349754

132

2

1

A2.bmp

0.161017

99

2

2

A2.bmp

0.257669

121

2

3

A2.bmp

0.315217

126

3

1

A1.bmp

0.112963

479

3

2

A1.bmp

0.331776

143

3

3

A1.bmp

0.358974

175

3

1

A2.bmp

0.085106

473

3

2

A2.bmp

0.373832

134

3

3

A2.bmp

0.243402

258

4

1

A1.bmp

0.237069

177

4

2

A1.bmp

0.420765

106

4

3

A1.bmp

0.504505

110

4

1

A2.bmp

0.305419

141

4

2

A2.bmp

0.357143

108

4

3

A2.bmp

0.569536

65

5

1

A1.bmp

0.358744

143

5

2

A1.bmp

0.438095

118

5

3

A1.bmp

0.421053

143

5

1

A2.bmp

0.333333

162

5

2

A2.bmp

0.330189

142

5

3

A2.bmp

0.296875

225

6

1

A1.bmp

0.435294

96

6

2

A1.bmp

0.449664

82

6

3

A1.bmp

0.5

50

6

1

A2.bmp

0.38835

126

6

2

A2.bmp

0.601266

63

6

3

A2.bmp

0.581818

46

1

1

B1.bmp

91

1

2

B1.bmp

32

1

3

B1.bmp

37

2

1

B1.bmp

29

2

2

B1.bmp

49

2

3

B1.bmp

71

3

1

B1.bmp

61

3

2

B1.bmp

71

3

3

B1.bmp

98

4

1

B1.bmp

55

4

2

B1.bmp

77

4

3

B1.bmp

112

5

1

B1.bmp

80

5

2

B1.bmp

92

5

3

B1.bmp

104

6

1

B1.bmp

74

6

2

B1.bmp

67

6

3

B1.bmp

50

1

1

B2.bmp

79

1

2

B2.bmp

33

1

3

B2.bmp

53

2

1

B2.bmp

19

2

2

B2.bmp

42

2

3

B2.bmp

58

3

1

B2.bmp

44

3

2

B2.bmp

80

3

3

B2.bmp

83

4

1

B2.bmp

62

4

2

B2.bmp

60

4

3

B2.bmp

86

5

1

B2.bmp

81

5

2

B2.bmp

70

5

3

B2.bmp

95

6

1

B2.bmp

80

6

2

B2.bmp

95

6

3

B2.bmp

64




V.Verification of effects of our parts




Name

Count

1

1

A1.bmp

0.330935

186

1

2

A1.bmp

0.259912

168

1

3

A1.bmp

0.310924

164

1

1

A2.bmp

0.260223

199

1

2

A2.bmp

0.18239

260

1

3

A2.bmp

0.268293

180

2

1

A1.bmp

0.244541

173

2

2

A1.bmp

0.19084

106

2

3

A1.bmp

0.546512

39

2

1

A2.bmp

0.215768

189

2

2

A2.bmp

0.232394

109

2

3

A2.bmp

0.597938

39

3

1

A1.bmp

0.590164

50

3

2

A1.bmp

0.359551

57

3

3

A1.bmp

0.280576

100

3

1

A2.bmp

0.504065

61

3

2

A2.bmp

0.411111

53

3

3

A2.bmp

0.178218

83

4

1

A1.bmp

0.392157

124

4

2

A1.bmp

0.57672

80

4

3

A1.bmp

0.536585

57

4

1

A2.bmp

0.406977

102

4

2

A2.bmp

0.433121

89

4

3

A2.bmp

0.415663

97

5

1

A1.bmp

0.383784

114

5

2

A1.bmp

0.379747

98

5

3

A1.bmp

0.438819

133

5

1

A2.bmp

0.370166

114

5

2

A2.bmp

0.20625

127

5

3

A2.bmp

0.365079

120

6

1

A1.bmp

0.322835

86

6

2

A1.bmp

0.303797

110

6

3

A1.bmp

0.35

65

6

1

A2.bmp

0.306569

95

6

2

A2.bmp

0.263566

95

6

3

A2.bmp

0.376147

68

7

1

A1.bmp

0.558252

91

7

2

A1.bmp

0.555556

52

7

3

A1.bmp

0.363265

156

7

1

A2.bmp

0.493976

84

7

2

A2.bmp

0.663866

40

7

3

A2.bmp

0.216981

166

8

1

A1.bmp

0.362069

111

8

2

A1.bmp

0.464646

53

8

3

A1.bmp

0.376623

96

8

1

A2.bmp

0.425

92

8

2

A2.bmp

0.541176

39

8

3

A2.bmp

0.458333

91

1

1

B1.bmp

92

1

2

B1.bmp

59

1

3

B1.bmp

74

2

1

B1.bmp

56

2

2

B1.bmp

25

2

3

B1.bmp

47

3

1

B1.bmp

72

3

2

B1.bmp

32

3

3

B1.bmp

39

4

1

B1.bmp

80

4

2

B1.bmp

109

4

3

B1.bmp

66

5

1

B1.bmp

71

5

2

B1.bmp

60

5

3

B1.bmp

104

6

1

B1.bmp

41

6

2

B1.bmp

48

6

3

B1.bmp

35

7

1

B1.bmp

115

7

2

B1.bmp

65

7

3

B1.bmp

89

8

1

B1.bmp

63

8

2

B1.bmp

46

8

3

B1.bmp

58

1

1

B2.bmp

70

1

2

B2.bmp

58

1

3

B2.bmp

66

2

1

B2.bmp

52

2

2

B2.bmp

33

2

3

B2.bmp

58

3

1

B2.bmp

62

3

2

B2.bmp

37

3

3

B2.bmp

18

4

1

B2.bmp

70

4

2

B2.bmp

68

4

3

B2.bmp

69

5

1

B2.bmp

67

5

2

B2.bmp

33

5

3

B2.bmp

69

6

1

B2.bmp

42

6

2

B2.bmp

34

6

3

B2.bmp

41

7

1

B2.bmp

82

7

2

B2.bmp

79

7

3

B2.bmp

46

8

1

B2.bmp

68

8

2

B2.bmp

46

8

3

B2.bmp

77