Difference between revisions of "Team:BCU/Parts"

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<h1>Parts</h1>
 
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
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<h3>Note</h3>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<h3>Adding parts to the registry</h3>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
 
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ADD PARTS
 
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<h3>Part Table </h3>
 
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
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<groupparts>iGEM18 BCU</groupparts>
 
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<span><a  href="https://2018.igem.org/Team:BCU">HOME</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Team">TEAM</a></span>
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<span>PROJECT</span>
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<div class="dropdown-content">
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<p><a href="https://2018.igem.org/Team:BCU/Description">Description</a></p>
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<span>EXPERIMENT</span>
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<p><a href="https://2018.igem.org/Team:BCU/Design">Design</a></p>
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<p><a href="https://2018.igem.org/Team:BCU/Improve">Materials&Reagents</a></p>
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<p><a href="https://2018.igem.org/Team:BCU/Demonstrate">Methods</a></p>
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<p><a href="https://2018.igem.org/Team:BCU/Results">Results</a></p>
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<span><a href="https://2018.igem.org/Team:BCU/Parts">PARTS</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/InterLab">INTERLAB</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Notebook">NOTEBOOK</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Attributions">ATTRIBUTION</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Model">MEDAL</a></span>
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<h1>Parts</h1>
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<h2>Basic Parts</h2>
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BBa_K2567000: nicA (Nicotine oxidoreductase)
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(http://parts.igem.org/wiki/index.php?title=Part:BBa_K2567000)
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The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation.
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We optimization the nicA in order to express protein successfully. Including but not limited to:
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• Codon usage bias
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• GC content
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• CpG dinucleotides content
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• mRNA secondary structure
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• Cryptic splicing sites
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• Premature PolyA sites
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• Internal chi sites and ribosomal binding sites
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• Negative CpG islands
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• RNA instability motif (ARE)
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• Repeat sequences (direct repeat, reverse repeat, and Dyad repeat)
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• Restriction sites that may interfere with cloning
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Additional sequences we propose to improve translational performance
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(1) To increase the efficiency of translational initiation
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• Kozak sequence
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• Shine-Dalgarno Sequence
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(2) To increase the efficiency of translational termination
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• Stop codon(TAA)
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Composite Parts
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BBa_K2567001: express nicA and degrade nicotine
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(http://parts.igem.org/Part:BBa_K2567001)
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Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine.
  
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Revision as of 10:21, 14 October 2018

无标题文档

HOME
TEAM
PROJECT

Description

EXPERIMENT

Design

Materials&Reagents

Methods

Results

PARTS
INTERLAB
NOTEBOOK
ATTRIBUTION
MEDAL

Parts

Basic Parts

BBa_K2567000: nicA (Nicotine oxidoreductase) (http://parts.igem.org/wiki/index.php?title=Part:BBa_K2567000) The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation. We optimization the nicA in order to express protein successfully. Including but not limited to: • Codon usage bias • GC content • CpG dinucleotides content • mRNA secondary structure • Cryptic splicing sites • Premature PolyA sites • Internal chi sites and ribosomal binding sites • Negative CpG islands • RNA instability motif (ARE) • Repeat sequences (direct repeat, reverse repeat, and Dyad repeat) • Restriction sites that may interfere with cloning Additional sequences we propose to improve translational performance (1) To increase the efficiency of translational initiation • Kozak sequence • Shine-Dalgarno Sequence (2) To increase the efficiency of translational termination • Stop codon(TAA) Composite Parts BBa_K2567001: express nicA and degrade nicotine (http://parts.igem.org/Part:BBa_K2567001) Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine.