Difference between revisions of "Team:TUDelft/Parts/PartsCollection"

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When constructing our BioBricks, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. We created a part collection comprised of 6 basic parts encoding for different EPO coding sequences, 2 basic parts encoding for the required components for targeted sequencing and 1 composite part: our fusion protein. All BioBricks are submitted with elaborate characterisation so the user may choose the best protein for their project accordingly. Our favourite basic part is <a href="http://parts.igem.org/Part:BBa_K2643001" class="grnbl" target="_blank">BBa_K2643001</a> and our favourite composite part is <a href="http://parts.igem.org/Part:BBa_K2643000" class="grnbl" target="_blank">BBa_K2643000</a>.
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<caption class="figgrnbl"><b>Table 1.</b> BioBrick Parts submitted by TU Delft iGEM 2018 team. All BioBricks were constructed by the team members involved in wetlab: Susan Bouwmeester, Kavish Kohabir, Venda Mangkusaputra, Timmy Paez, Lisbeth Schmidtchen and Nicole Bennis</caption>
  
 
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Revision as of 11:04, 14 October 2018

partcollection

Part Collection

When constructing our BioBricks, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. We created a part collection comprised of 6 basic parts encoding for different EPO coding sequences, 2 basic parts encoding for the required components for targeted sequencing and 1 composite part: our fusion protein. All BioBricks are submitted with elaborate characterisation so the user may choose the best protein for their project accordingly. Our favourite basic part is BBa_K2643001 and our favourite composite part is BBa_K2643000.

Table 1. BioBrick Parts submitted by TU Delft iGEM 2018 team. All BioBricks were constructed by the team members involved in wetlab: Susan Bouwmeester, Kavish Kohabir, Venda Mangkusaputra, Timmy Paez, Lisbeth Schmidtchen and Nicole Bennis
Fave Part Name Type Description Designer
♥ BBa_K2643000 Composite dxCas9-linker-Tn5 fusion protein (HIS Tag) TU Delft 2018
♥ BBa_K2643004 Basic Coding region (CDS) of human erythropoietin (EPO) hormone TU Delft 2018
BBa_K2643005 Basic Human EPO gene with artificial intron (615 bp) TU Delft 2018
BBa_K2643006 Basic Human EPO gene with artificial intron (135 bp) TU Delft 2018
BBa_K2643007 Basic Human EPO gene with 2 artificial introns (615 + 135 bp) TU Delft 2018
BBa_K2643008 Basic Human EPO gene with mutation 1 (5 bp) TU Delft 2018
BBa_K2643009 Basic Human EPO gene with mutation 2 (12 bp) TU Delft 2018
BBa_K2643011 Basic Mosaic Ends-flanked Kanamycin cassette and RFP TU Delft 2018
BBa_K2643012 Basic T7p expressing sgRNA targeting the EPO coding sequence TU Delft 2018