Difference between revisions of "Team:NUS Singapore-A/Demonstrate"
| Line 30: | Line 30: | ||
<h2>BLUE LIGHT REPRESSIBLE SYSTEM</h2> | <h2>BLUE LIGHT REPRESSIBLE SYSTEM</h2> | ||
| − | <p>Improved characterization of EL222 blue light repressible promoter, | + | <p>Improved characterization of EL222 blue light repressible promoter, P<sub>BLrep</sub> (BBa_)</p> |
| − | <p>Improved characterization of EL222 blue light inducible promoter, | + | <p>Improved characterization of EL222 blue light inducible promoter, P<sub>BLind</sub> (BBa_)</p> |
<p>Demonstrated blue light repression of luteolin production</p> | <p>Demonstrated blue light repression of luteolin production</p> | ||
| Line 37: | Line 37: | ||
<p>Constructed a stress reporter module</p> | <p>Constructed a stress reporter module</p> | ||
| − | <p>Characterized the burden responsive promoter, | + | <p>Characterized the burden responsive promoter, P<sub>htpG1</sub></p> |
<p>Demonstrated stress induced by naringenin- and luteolin-producing plasmids</p> | <p>Demonstrated stress induced by naringenin- and luteolin-producing plasmids</p> | ||
Revision as of 13:20, 14 October 2018
Demonstrate
Gold Medal Criterion #4
XYLOSE AS FEEDSTOCK
Successfully constructed an inducible xylose-utilizing module
Demonstrated improved growth of E. coli BL21* containing this module in xylose and glucose-xylose mixture
DE NOVO BIOSYNTHESIS
Successfully constructed naringenin-producing plasmid with just a single missing enzyme required for full de novo synthesis
Demonstrated production of naringenin from coumaric acid in E. coli Acella and BL21*
LUTEOLIN
Successfully constructed chemically-inducible and light inducible luteolin-producing plasmid
Demonstrated production of luteolin from naringenin in E. coli BL21*
Characterized Bba_ (F3’H), Bba (FNS), Bba (pBAD-FNS)
BLUE LIGHT REPRESSIBLE SYSTEM
Improved characterization of EL222 blue light repressible promoter, PBLrep (BBa_)
Improved characterization of EL222 blue light inducible promoter, PBLind (BBa_)
Demonstrated blue light repression of luteolin production
STRESS REPORTER
Constructed a stress reporter module
Characterized the burden responsive promoter, PhtpG1
Demonstrated stress induced by naringenin- and luteolin-producing plasmids
CELL-MACHINE INTERFACE
Designed and built devices which help characterize optogenetic circuits in petri dishes and 250 ml conical flasks
Designed and built a 500 ml working volume benchtop optogenetic bioreactor, which comprises a peristaltic pump, 2-in-1 OD and fluorescence sensor, and fermentation chamber
Designed and implemented a feedback control system to control the optogenetic bioreactor
Please see the 2018 Medals Page for more information.