Difference between revisions of "Team:NUS Singapore-A/Demonstrate"
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<h2>XYLOSE AS FEEDSTOCK</h2> | <h2>XYLOSE AS FEEDSTOCK</h2> | ||
| − | <p> | + | <p>✔Successfully constructed an inducible xylose-utilizing module</p> |
| − | <p> | + | <p>✔Demonstrated improved growth of <i>E. coli</i> BL21* containing this module in xylose and glucose-xylose mixture</p> |
<h2>DE NOVO BIOSYNTHESIS</h2> | <h2>DE NOVO BIOSYNTHESIS</h2> | ||
| − | <p> | + | <p>✔Successfully constructed a naringenin-producing plasmid with just a single missing enzyme required for full de novo synthesis</p> |
| − | <p> | + | <p>✔Demonstrated production of naringenin from coumaric acid in E. coli Acella and BL21*</p> |
<h2>LUTEOLIN</h2> | <h2>LUTEOLIN</h2> | ||
| − | <p> | + | <p>✔Successfully constructed chemically-inducible and light inducible luteolin-producing plasmids</p> |
| − | <p> | + | <p>✔Demonstrated production of luteolin from naringenin in E. coli BL21*</p> |
| − | <p> | + | <p>✔Characterized Bba_ (F3’H), Bba (FNS), Bba (pBAD-FNS)</p> |
<h2>BLUE LIGHT REPRESSIBLE SYSTEM</h2> | <h2>BLUE LIGHT REPRESSIBLE SYSTEM</h2> | ||
| − | <p> | + | <p>✔Improved characterization of EL222 blue light repressible promoter, P<sub>BLrep</sub> (BBa_)</p> |
| − | <p> | + | <p>✔Improved characterization of EL222 blue light inducible promoter, P<sub>BLind</sub> (BBa_)</p> |
| − | <p> | + | <p>✔Demonstrated blue light repression of luteolin production</p> |
<h2>STRESS REPORTER</h2> | <h2>STRESS REPORTER</h2> | ||
| − | <p> | + | <p>✔Successfully constructed a stress reporter module</p> |
| − | <p> | + | <p>✔Characterized the burden responsive promoter, P<sub>htpG1</sub></p> |
| − | <p> | + | <p>✔Demonstrated stress induced by naringenin- and luteolin-producing plasmids</p> |
<h2>CELL-MACHINE INTERFACE</h2> | <h2>CELL-MACHINE INTERFACE</h2> | ||
| − | <p> | + | <p>✔Designed and built devices which help characterize optogenetic circuits in petri dishes and 250 ml conical flasks</p> |
| − | <p> | + | <p>✔Designed and built a 500 ml working volume benchtop optogenetic bioreactor, which comprises a peristaltic pump, 2-in-1 OD and fluorescence sensor, and fermentation chamber</p> |
| − | <p> | + | <p>✔Designed and implemented a feedback control system to control the optogenetic bioreactor</p> |
</p> | </p> | ||
Revision as of 13:21, 14 October 2018
Demonstrate
Gold Medal Criterion #4
XYLOSE AS FEEDSTOCK
✔Successfully constructed an inducible xylose-utilizing module
✔Demonstrated improved growth of E. coli BL21* containing this module in xylose and glucose-xylose mixture
DE NOVO BIOSYNTHESIS
✔Successfully constructed a naringenin-producing plasmid with just a single missing enzyme required for full de novo synthesis
✔Demonstrated production of naringenin from coumaric acid in E. coli Acella and BL21*
LUTEOLIN
✔Successfully constructed chemically-inducible and light inducible luteolin-producing plasmids
✔Demonstrated production of luteolin from naringenin in E. coli BL21*
✔Characterized Bba_ (F3’H), Bba (FNS), Bba (pBAD-FNS)
BLUE LIGHT REPRESSIBLE SYSTEM
✔Improved characterization of EL222 blue light repressible promoter, PBLrep (BBa_)
✔Improved characterization of EL222 blue light inducible promoter, PBLind (BBa_)
✔Demonstrated blue light repression of luteolin production
STRESS REPORTER
✔Successfully constructed a stress reporter module
✔Characterized the burden responsive promoter, PhtpG1
✔Demonstrated stress induced by naringenin- and luteolin-producing plasmids
CELL-MACHINE INTERFACE
✔Designed and built devices which help characterize optogenetic circuits in petri dishes and 250 ml conical flasks
✔Designed and built a 500 ml working volume benchtop optogenetic bioreactor, which comprises a peristaltic pump, 2-in-1 OD and fluorescence sensor, and fermentation chamber
✔Designed and implemented a feedback control system to control the optogenetic bioreactor
Please see the 2018 Medals Page for more information.