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   <h4 align="justify">June 15<sup>th</sup><br>Center for Research in Food and Development A.C. (CIAD) visit</h4><br>
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   <h4 align="justify">June 27<sup>th</sup><br>Meeting with MVZ Ernesto Tanús Sánchez, MVZ Adolfo Arroyo, and Ing. José Luis González Miranda.</h4><br>
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   <div class="a"><p align="justify">The main objective of the realization of this visit was to get to know and evaluate the ideal conditions with which <i>M. plutonius</i> and <i>P. larvae</i> grow and how are they handled <i>in vitro</i>. On another hand, we looked forward to knowing the practices of in vitro bee breeding.</p></div><br>
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   <div class="a"><p align="justify">For us, it was very important to receive feedback from specialists and be able to exchange opinions about our project. After the video call we had with Ernesto Tanús, we were able to keep in contact with him, since he was visiting the city of Chihuahua we took advantage of the opportunity and we scheduled a meeting that was also attended by the President of the National Association of Veterinary Doctors Specialists in Bees (ANMVEA) (MVZ Adolfo Arroyo) and a beekeeping technician from the Secretary of Agriculture, Livestock, Rural Development, Fishing and Food (SAGARPA) (Ing. José Luis González Miranda). The legal framework consultant accompanied by two active members of human practices attended this meeting that lasted approximately 1h 30. We were looking forward to presenting our project, for them to the give us their general insights, discuss the considerations of legal aspects that involve our project, and receive counseling of the bee's anatomy.</p></div></div>
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  <div class="a"><p align="justify">Four members of the team had the opportunity to travel to Cuauhtémoc and attend this visit where we invested approximately four hours, first in the laboratory and then in the field. M. S. Alejandro Romo Chacón (who has participated in more than 20 scientific &nbsp;dissemination &nbsp;articles &nbsp;related &nbsp;to &nbsp;bees or</p></div></div>
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<p align="justify">agriculture) welcomed us to the facilities and once we entered the laboratory he began to explain us about the bees’ life cycle and about some of the products and instruments beekeepers use to handle hives. &nbsp;Later, we &nbsp;were &nbsp;able &nbsp;to &nbsp;meet M.S.</p>
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      <p align="justify"> Adrián Ponce de León, who is researching about foulbrood bacteria, and along with M.S. Romo, he explained us about procedures and safety considerations in order to manage the in vitro growth of <i>P. larvae</i> and <i>M. plutonius</i>.</p><br>
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      <div class="a"><p align="justify">The day of our visit, M.S. Romo and M.S. Ponce de León planned to go to the bee field and carry out bee larvae transferences, for its following in vitro breeding. This activity is one of the parts that implies our project once we reach the experimentation stage with the insects and M.S. Romo and M.S. Ponce de León attentively invited us to accompany them to the field, they even lent us suits and veils to interact &nbsp;directly &nbsp;with &nbsp;the &nbsp;bees! &nbsp;Previously &nbsp;we &nbsp;had &nbsp;consulted &nbsp;a</p></div></div>
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      <p align="justify">protocol that showed us all the details about the in vitro breeding of bees and by observing the techniques used by Ponce de León for the transference, we were able to validate and somehow, to begin with, the practice of this activity.</p><br>
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  <div class="a"><p align="justify">Interacting with the bees and being able to observe the composition of the bee boxes, the way in which the different foods were applied and the way in which beekeepers approached the bees were all key elements that allowed us to define the optimum application method of our product and helped us ensure its appropriate integration regarding each characteristic of the hive organization and the work done by beekeepers.</p></div><br>
 
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  <div class="a"><p align="justify">When returning to the CIAD facilities we had the opportunity to discuss the use of bees in the laboratory, how many bees were used? What was this number based on? Were the &nbsp;three &nbsp;R’s considered? The</p></div></div>
 
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<p align="justify">reality is that in Mexico there does not exist such regulations and the bioethical part of working with bees in the laboratory is quite unattended. We found this situation somewhat unsettling and it led us to perform a more extensive analysis of this topic. (consult the bioethical essay in)</p><br>
 
  
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<div class="a"><p align="justify">M.S. Romo and M.S. Ponce de León could broaden their panorama on the research that is currently being done with bees and their diseases thanks to the sharing we made with them of our project and the allusion that synthetic biology is its fundamental part.</p></div><br>
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<div class="a"><p align="justify">Thanks to this visit we were able to have our first real approach with bees, we knew the reality of working with them and working with the two strains according to a specialized laboratory that has already experienced the proper biosafety considerations. This visit gave us different data that is very useful for the whole experimental plan of our project, and besides that, we lived a little of what beekeepers live every day, like traveling inside the truck with many bees that stayed inside from the field and even experiencing some bee-stings. We were also able to capture amazing photos of the hives!</p></div><br>
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<h4 align="justify">References</h4>
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<li>Image retrieved from: Grupo Caresner S. de R.L de C.V. (2018). Museo Menonita. https://www.mexicodestinos.com/chihuahua/tours/museo-menonita.html</li>
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Revision as of 14:44, 14 October 2018

Erwinions
















June 27th
Meeting with MVZ Ernesto Tanús Sánchez, MVZ Adolfo Arroyo, and Ing. José Luis González Miranda.


For us, it was very important to receive feedback from specialists and be able to exchange opinions about our project. After the video call we had with Ernesto Tanús, we were able to keep in contact with him, since he was visiting the city of Chihuahua we took advantage of the opportunity and we scheduled a meeting that was also attended by the President of the National Association of Veterinary Doctors Specialists in Bees (ANMVEA) (MVZ Adolfo Arroyo) and a beekeeping technician from the Secretary of Agriculture, Livestock, Rural Development, Fishing and Food (SAGARPA) (Ing. José Luis González Miranda). The legal framework consultant accompanied by two active members of human practices attended this meeting that lasted approximately 1h 30. We were looking forward to presenting our project, for them to the give us their general insights, discuss the considerations of legal aspects that involve our project, and receive counseling of the bee's anatomy.


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