Attributions

• PI's
  Jan Kok

  Dirk-Jan Scheffers
• Instructors
  Andreas Milias

  Linda Dijkshoorn

  Agnieszka Wegrzyn

  Sergey Lunev
• General Support
  Lisbeth Ravnkilde Kjolbye: for providing us with many contacts to get our project off the ground

  Thanks to Matthijs’ personnel pass for free coffee

  Sonja Billerbeck: advice about the iGEM competition in general

  Siger Holsappel: general support in logistics

  Ger Telkamp: for providing us with lab-equipment from the practicum pool

  Manon Dusseljee: for financial administrative support

  Hannah Dosenko: for taking our photos and group picture

  Winand Slingenbergh and Fablab Groningen: for helping us with our 3D print designs
• Fundraising help and advice
  Chienes Metus: For aiding us in our financial planning and taking care of all of our bills

  Mareike Schmidt: For giving advice on organizing our fundraising barbecue

  Derk Bosker: For guidance in the crowd-funding page
• Lab Support
  Tau Jang: for providing us with A. Thaliana for extracting the PAL2 and general lab advice

  Jeroen Nijland: for guiding us with CRISPR-Cas9 and providing us with an extra lab and protocols and giving access to HPLC system

  Arnold Driessen: for advice about the use of CRISPR-Cas9 and evolution experiments in our project and lab access

  Jan Kiel: for helping us setting up our lab and providing us with cellulose

  Wiktor Szymanski: for advice about phosphorylation of cellulose and for allowing us to use space and materials in their laboratory

  Dirk Jan Slotboom: for providing us with access to a plate reader for the Interlab study

  Aditya Iyer: for help in the Interlab plate-reader experiments and supply of lab materials

  Hemant Kumar: for guidance in the cellulase activity assays & advise how to improve the HPLC protocol for the Bordeaux collaboration

  Alisa Garaeva: for providing us with a plasmid for our collaboration with iGEM Leiden

  Amanda van Tilburg: for delivery of primers and donation of BL21(DE3) strain

  Buu Minh Tran: for providing us with a plasmid for our collaboration with iGEM Leiden

  Vakhil Takhaveev: for being so kind to already transform these plasmids in E. coli for us

  Jakob Viel: for helping us find -80 freezer space, and locating some chemicals

  Marisya Majsnerowska: for providing us with materials to visualise our His-tagged proteins on Western blots

  Andre Boltjes: for feedback on the styrene extraction protocol

  Ruben Maaskant: for helping with the GC-MS and GC-FID methods

  Arjen Krikken: for providing strains, plasmids, and some restriction enzymes

  Huala Wu: for providing YPD Zeocin plates

  Anna Lauxen: for instructions on the Tecan Geneos platereader

  Niels de Kok: for providing us with E. coli DH5a and BL21 (DE3)

  Marten Exterkate: for cloning advice

  László Mózsik: for providing a kanamycin stock and general lab advice

  Chris Williams: for providing general tips and zeocin

  Jhonatan Hernandez-Valdes: for instructions on the Thermo-Scientific Varioskan LUX platereader

  Luiza Morawska: for instructions on the Olympus DeltaVision fluorescence microscope
• Lab design Support
  Bert Poolman: for giving us advice on how to export styrene from the cell

  Jan Willem Meints: for helping us develop a HPLC quantification method for styrene

  Marco Fraaije: for advice about a cellulase-activity assay

  Dick Janssen: For advice on experimental design of the enzymatic cascade
• (Integrated) Human Practices and Public Engagement Support
  Karin Ree: for giving us tips on who to contact

  Tjerk Douwma: for helping us doing carbon footprint analysis (Life Cycle Analysis)

  Katja Loos: for advice on biodegradable plastics

  Francesco Picchioni: for advise on what plastic to go for

  Martin van Dord: for advice about plastics and rubbers (NRK)

  Pieter Imhof: for advice about the production of biochemicals (BioBTX)

  Machiel van Steenis (Program manager Innovative Biomass Applications): for advice about the application of biomass-based technologies

  Gert-Jan Euverink: for advice about the use of sewage material as a source of our cellulose and technical improvements for our yeast strain

  Frans Timmermans: for helping us extrapolate our project to alternative biomass sources (algae as cellulose source)

  Andre Fay: for sharing his expertise in the field of biomass

  Korienke Smit and Niek Savelkoul: for coaching us about integrated human practices (RIVM)

  ScienceLinx: for helping us with education and public engagement

  Scholierenacademie: for giving us the opportunity to educate primary school students

  Paddepoel: for giving us a stage for our outreach event to educate the general population on science and our project in general

  Girbe Buist: for supplying us with materials for our outreach event in Paddepoel

  Jeroen Tieleman (Bioclear Earth): for giving us tips on the resource of biomass
• Outreach and Social Media
  Daniel Oostenbrink: for helping us fine-tune our animation’s script

  Joris-Jan Coenraads: for lending us his microphone to record our animation
• Drylab Support
  Agnieszka Wegrzyn: for general modelling advice and advice on flux based modelling

  Matthias Heinemann: for giving us advice on modelling our project using Flux balance Analysis

  Siewert-Jan Marrink: for discussing modelling approaches with us

  Pim Frederix: for giving us a group workshop on Molecular Dynamics

  Paolo Telles de Souza: for helping to setup the cellulose binding molecular dynamics model