Difference between revisions of "Team:NDC-HighRiverAB/Parts"

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Revision as of 16:15, 14 October 2018

PARTS

This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:

- First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in pseudomonas aeruginosa. (Part:BBa_K2694001)

- For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli. (Part:BBa_R0011)

- The RBS we used is B0034. (Part:BBa_B0034)

- Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part:BBa_B0015)

- After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000.