Difference between revisions of "Team:Bio Without Borders/Experiments"
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<p> 1) Thaw on ice. Add 5 micro of Dna (one ligation one control) sit for 30 min. </p> | <p> 1) Thaw on ice. Add 5 micro of Dna (one ligation one control) sit for 30 min. </p> | ||
<p> 2) Heat shock at 42C for 30 seconds. </p> | <p> 2) Heat shock at 42C for 30 seconds. </p> | ||
Revision as of 19:47, 20 June 2018
Experiments
Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.
Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
Transformation Test
1) Thaw on ice. Add 5 micro of Dna (one ligation one control) sit for 30 min.
2) Heat shock at 42C for 30 seconds.
3) Add 950 uL of SOC (from fridge).
4) Spread 50-100uL of cells and ligation mixtures onto the plates.
5) Incubate overnight at 37C.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project