Difference between revisions of "Team:Lambert GA/Experiments"

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Click on the step to be directed to the procedure!
 
Click on the step to be directed to the procedure!
  
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<b style="font-size:16px;">
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<b style="font-size:20px;">
 
<ol>
 
<ol>
 
<li><a style="color:black; font-size:16px;" href="#Step1">Miniprep/Nanodrop</a></li>
 
<li><a style="color:black; font-size:16px;" href="#Step1">Miniprep/Nanodrop</a></li>
 
<li><a style="color:black; font-size:16px;" href="#Step2">Digest</a></li>
 
<li><a style="color:black; font-size:16px;" href="#Step2">Digest</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step3">Gel</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step4">Ligation</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step5">Transformation/electroporation, Plate</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step6">Colony PCR (Screening)</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step7">Gel</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step8">Inoculate correct colony to a liquid culture</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step9">Miniprep/Nanodrop</a></li>
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<li><a style="color:black; font-size:16px;" href="#Step10">Sequence</a></li>
 
</ol>
 
</ol>
 
</b>
 
</b>
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<br><br>
 
<br><br>
 
<div id="content2">
 
<div id="content2">
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Miniprep: grown culture, microcentrifuge, 2 1.5mL microcentrifuge tubes, mini column and collection tube, Solution I, Solution II, Solution III, HBC Wash Buffer, DNA Wash Buffer, Elution Buffer, micropipette and tips
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<b>Miniprep:</b> grown culture, microcentrifuge, 2 1.5mL microcentrifuge tubes, mini column and collection tube, Solution I, Solution II, Solution III, HBC Wash Buffer, DNA Wash Buffer, Elution Buffer, micropipette and tips
 
<br>
 
<br>
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Nanodrop: nanodrop machine, miniprepped DNA, Kimtech wipes, micropipette and tips
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<b>Nanodrop:</b> nanodrop machine, miniprepped DNA, Kimtech wipes, micropipette and tips
 
<br>
 
<br>
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Digest: miniprepped DNA, dH₂O, 10X RE-Mix, standard restriction enzyme, micropipettes and tips
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<b>Digest:</b> miniprepped DNA, dH₂O, 10X RE-Mix, standard restriction enzyme, micropipettes and tips
 
<br>
 
<br>
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Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
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<b>Gel:</b> agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
 
<br>
 
<br>
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Ligation: vector, parts 1 and 2, ligase buffer, ligase, Antarctic phosphatase, microcentrifuge tube, ice, micropipette and tips  
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<b>Ligation:</b> vector, parts 1 and 2, ligase buffer, ligase, Antarctic phosphatase, microcentrifuge tube, ice, micropipette and tips  
 
<br>
 
<br>
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Transformation: ice, ligation mixture, competent cells, incubator, LB media, microcentrifuge tubes, micropipette and tips Plate: agar plate, micropipette and tips, beads
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<b>Transformation:</b> ice, ligation mixture, competent cells, incubator, LB media, microcentrifuge tubes, micropipette and tips Plate: agar plate, micropipette and tips, beads
 
<br>
 
<br>
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Colony PCR: dH₂O, buffer, VF₂, VR, Q5 polymerase, dNTP, DNA dilution, micropipette and tips, PCR tubes, thermocycler, ice  
+
<b>Colony PCR:</b> dH₂O, buffer, VF₂, VR, Q5 polymerase, dNTP, DNA dilution, micropipette and tips, PCR tubes, thermocycler, ice  
 
<br>
 
<br>
−
Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
+
<b>Gel:</b> agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
 
<br>
 
<br>
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Inoculate: LB media, dilution, micropipette and tips
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<b>Inoculate:</b> LB media, dilution, micropipette and tips
  
 
</div>
 
</div>
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<div id="Step2"></div>
 
<div id="Step2"></div>
  
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<div id="Step3"></div>
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<div id="Step4"></div>
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<div id="Step5"></div>
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<div id="Step6"></div>
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<div id="Step7"></div>
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<div id="Step8"></div>
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<div id="Step9"></div>
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<div id="Step10"></div>
  
 
</div>
 
</div>

Revision as of 00:52, 15 October 2018

CALM ELECTROPEN
BACKGROUND DATA & METHODS RESULTS DISCUSSION COLOR Q SUPPLEMENTARY REFERENCES
COLOR Q CHROME Q REFERENCES
PRINCIPLES & DESIGN MODEL & THEORY PRODUCT DESIGN REFERENCES
INTEGRATED HUMAN PRACTICES EDUCATION & ENGAGEMENT COLLABORATIONS
BASIC PARTS COMPOSITE PARTS
DESCRIPTION BACKGROUND DESIGN EXPERIMENTS LAB NOTEBOOK INTERLAB RESULTS DEMONSTRATE THE VISION
TEAM MEMBERS ATTRIBUTIONS
E X P E R I M E N T S



































Wet Lab Summary


Gaurav add wet lab summary


Work Flow




Materials


Miniprep: grown culture, microcentrifuge, 2 1.5mL microcentrifuge tubes, mini column and collection tube, Solution I, Solution II, Solution III, HBC Wash Buffer, DNA Wash Buffer, Elution Buffer, micropipette and tips
Nanodrop: nanodrop machine, miniprepped DNA, Kimtech wipes, micropipette and tips
Digest: miniprepped DNA, dH₂O, 10X RE-Mix, standard restriction enzyme, micropipettes and tips
Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
Ligation: vector, parts 1 and 2, ligase buffer, ligase, Antarctic phosphatase, microcentrifuge tube, ice, micropipette and tips
Transformation: ice, ligation mixture, competent cells, incubator, LB media, microcentrifuge tubes, micropipette and tips Plate: agar plate, micropipette and tips, beads
Colony PCR: dH₂O, buffer, VF₂, VR, Q5 polymerase, dNTP, DNA dilution, micropipette and tips, PCR tubes, thermocycler, ice
Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
Inoculate: LB media, dilution, micropipette and tips