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{{iTesla-SoundBio}} | {{iTesla-SoundBio}} | ||
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+ | <h1>Recording Our Progress | ||
+ | <script> | ||
+ | $("#title").text("Lab Notebook") | ||
+ | </script> | ||
+ | </h1> | ||
+ | |||
+ | <body> | ||
<style> | <style> | ||
− | + | h1 { | |
− | font: | + | font: 600 1.5em/1 'Raleway', sans-serif; |
color: rgba(0,0,0,.5); | color: rgba(0,0,0,.5); | ||
text-align: center; | text-align: center; | ||
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width: 100%; | width: 100%; | ||
− | padding-top: | + | padding-top: 50px; |
− | padding-bottom: | + | padding-bottom: 50px; |
} | } | ||
− | + | .column { | |
− | + | float: left; | |
− | + | width: 30%; | |
− | + | padding: 15px; | |
− | + | } | |
− | + | .protocols { | |
− | + | margin: 5px; | |
+ | border: 1px solid #ccc; | ||
+ | float: left; | ||
+ | width: 20%; | ||
} | } | ||
− | + | .protocols:hover { | |
− | + | border: 1px solid #777; | |
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− | + | ||
} | } | ||
− | + | .protocols img { | |
− | + | width: 100%; | |
− | + | height: auto; | |
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} | } | ||
+ | .desc { | ||
+ | padding: 15px; | ||
+ | text-align: center; | ||
+ | } | ||
</style> | </style> | ||
− | |||
− | < | + | <div class="column"> |
+ | <h2> | ||
+ | <p><strong>Date:</strong> 2018/06/02</p> | ||
+ | <a href=" https://static.igem.org/mediawiki/2018/3/3e/T--iTesla-SoundBio--Lab_Notebook_2018-6-2.pdf">Transform blue coral amilCP into already competent dh5 alpha cells</a> | ||
− | < | + | <p><strong>Date:</strong> 2018/06/08</p> |
− | <a href="https:// | + | <a href=" https://static.igem.org/mediawiki/2018/3/3c/T--iTesla-SoundBio--Lab_Notebook_2018-6-8.pdf"> PCR Practice and E. Coli Culturing</a> |
− | + | ||
− | < | + | <p><strong>Date:</strong> 2018/06/09</p> |
− | <a href="https:// | + | <a href="https://static.igem.org/mediawiki/2018/7/70/T--iTesla-SoundBio--Lab_Notebook_2018-6-9.pdf"> Miniprep Integration vector and BBa_k608006 (biobricking backbone) for future |
− | </ | + | digestion and ligation. Also, to run an analytical gel on last night’s PCR to analyze its success. </a> |
− | < | + | <p><strong>Date:</strong> 2018/06/10</p> |
− | <a href="https://2018.igem.org/ | + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--iTesla-SoundBio--Lab_Notebook_2018-6-8.pdf"> |
− | </ | + | Repeat Miniprep of Biobrick Backbone </a> |
+ | |||
+ | <p><strong>Date:</strong> 2018/06/11</p> | ||
+ | <a href="https://static.igem.org/mediawiki/2018/a/a4/T--iTesla-SoundBio--Lab_Notebook_2018-6-11.pdf"> | ||
+ | Redo PCR of gblocks and Redo Analytical Gel on First PCR</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/06/16</p> | ||
+ | <a href="https://static.igem.org/mediawiki/2018/7/77/T--iTesla-SoundBio--Lab_Notebook_2018-6-16.pdf"> | ||
+ | Digest of Miniprepped Biobricking Backbone and Analytical Gel</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/06/17</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/4/46/T--iTesla-SoundBio--Lab_Notebook_2018-6-17.pdf"> | ||
+ | Gel Purifying Digested BB Backbone slices and Digesting PCR'd FC 1 </a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/06/21</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/1/14/T--iTesla-SoundBio--Lab_Notebook_2018-6-21.pdf"> | ||
+ | Second Miniprep of Biobricking Backbone & Integration Vector of B. Subtilis and testing PCRs with phusion stuff AND test-digest for SacII and BglII</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/06/22</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/2/21/T--iTesla-SoundBio--Lab_Notebook_2018-6-22.pdf"> | ||
+ | Gel Test of psb1a3, pax01, and test-digest for BglII/SacII. BB digest of psb1a3, and purification gel</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/06/30</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/f/f2/T--iTesla-SoundBio--Lab_Notebook_2018-6-30.pdf"> | ||
+ | Gel Extraction of psb1A3, Ligation of FC Frag 1 and psb1a3, and Transformation of Ligated Plasmid into E.coli</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/05</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/6/6d/T--iTesla-SoundBio--Lab_Notebook_2018-7-5.pdf"> | ||
+ | Digesting, running a purification gel and gel extracting miniprepped psb1a3 </a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/06</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/3/3a/T--iTesla-SoundBio--Lab_Notebook_2018-7-6.pdf"> | ||
+ | Miniprepping psb1a3 (Round #3)</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/07</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/b/b7/T--iTesla-SoundBio--Lab_Notebook_2018-7-7.pdf"> | ||
+ | Digesting and gel purifying psb1a3, PCRing Frag 1</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/12</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/9/99/T--iTesla-SoundBio--Lab_Notebook_2018-7-12.pdf"> | ||
+ | Gel extracting digested psb1a3, PCRing and PCR purifying Factor C Frag 2</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/14</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/f/fa/T--iTesla-SoundBio--Lab_Notebook_2018-7-14.pdf"> | ||
+ | Digest psb1a3, gel purify, gel extract and nanodrop digested psb1a3</a> | ||
− | < | + | <p><strong>Date:</strong> 2018/07/16</p> |
− | <a href="https:// | + | <a href= "https://static.igem.org/mediawiki/2018/3/33/T--iTesla-SoundBio--Lab_Notebook_2018-7-16.pdf"> |
+ | Digesting Frag 1, Ligating Frag 1 and Digested psb1a3, Transforming into E.coli, Transforming Interlab plasmids into E.coli, and Miniprepping psb1a3 </a> | ||
+ | </h2> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="column"> |
− | <a href="https://2018.igem.org/ | + | <h2> |
+ | |||
+ | |||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/20</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/e/ef/T--iTesla-SoundBio--Lab_Notebook_2018-7-20.pdf"> | ||
+ | ESETITTTTTTTTTTTTTTTTTTTTTTTTT </a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/21</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/e/ec/T--iTesla-SoundBio--Lab_Notebook_2018-7-21.pdf"> | ||
+ | PCRing more Fragment 1, Running an analytical gel, digesting psb1a3 and psb1c3, running a purificaiton gel, gel extracting and nanodropping resulting digests</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/22</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/2/2c/T--iTesla-SoundBio--Lab_Notebook_2018-7-22.pdf"> | ||
+ | Miniprepping psb1a3 and psb1c3, digesting, running a purification gel and gel extracting</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/27</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/3/34/T--iTesla-SoundBio--Lab_Notebook_2018-7-27.pdf"> | ||
+ | Digesting Frag 1, Ligating Frag 1 and psb1a3/c3, Transforming into E.coli</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/29</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/0/03/T--iTesla-SoundBio--Lab_Notebook_2018-7-29.pdf"> | ||
+ | More PCRs of Fragment 1</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/30</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/f/f9/T--iTesla-SoundBio--Lab_Notebook_2018-7-30.pdf"> | ||
+ | Miniprep, digest, and gel purification of pSB1C3, pcr and pcr purification of fragment 1 geneblock</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/07/31</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/b/b5/T--iTesla-SoundBio--Lab_Notebook_2018-7-31.pdf"> | ||
+ | Miniprepping psb1a3 (Round #3)</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/01</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/7/73/T--iTesla-SoundBio--Lab_Notebook_2018-8-1.pdf"> | ||
+ | Running a Purification Gel on Digested FC 1 NOT SUCCESSFUL</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/02</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/1/14/T--iTesla-SoundBio--Lab_Notebook_2018-8-2.pdf"> | ||
+ | Purification Gel of psb1C3</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/03</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/6/6c/T--iTesla-SoundBio--Lab_Notebook_2018-8-3.pdf"> | ||
+ | PCR fragment 1</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/07</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/6/6b/T--iTesla-SoundBio--Lab_Notebook_2018-8-07.pdf"> | ||
+ | PCR, Digest Fragment 1 & Digest psb1C3</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/08</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/4/42/T--iTesla-SoundBio--Lab_Notebook_2018-8-8.pdf"> | ||
+ | Debugging PCR using psb1C3 and DMSO</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/09</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/a/a4/T--iTesla-SoundBio--Lab_Notebook_2018-8-9.pdf"> | ||
+ | PCR'ing Fragment 2, Digesting psb1C3</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/10</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/8/8e/T--iTesla-SoundBio--Lab_Notebook_2018-8-10.pdf"> | ||
+ | Gel extraction of digested fragment 2</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/11</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/3/3f/T--iTesla-SoundBio--Lab_Notebook_2018-8-11.pdf.pdf"> | ||
+ | Analytical gel of frag 1 PCRs and gel extraction of fragment 2 digests</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/12</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/3/39/T--iTesla-SoundBio--Lab_Notebook_2018-8-12.pdf"> | ||
+ | Ligation of pSB1C3 + frag 2 and transformation</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/21</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/1/18/T--iTesla-SoundBio--Lab_Notebook_2018-8-21.pdf"> | ||
+ | Miniprep of pSB1C3+mRFP, PCR of fragment 2 and fragment 1</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/22</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/e/ee/T--iTesla-SoundBio--Lab_Notebook_2018-8-22.pdf"> | ||
+ | PCR of fragments 1, 2, and pSB1C3 and analytical gel</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/26</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/8/80/T--iTesla-SoundBio--Lab_Notebook_2018-8-26.pdf"> | ||
+ | PCR of fragments 1 and 2</a> | ||
+ | |||
+ | </h2> | ||
</div> | </div> | ||
− | </ | + | <div class="column"> |
− | </ | + | <h2> |
+ | |||
+ | <p><strong>Date:</strong> 2018/08/27</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/b/b8/T--iTesla-SoundBio--Lab_Notebook_2018-8-27.pdf"> | ||
+ | Practice with bacillus</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/29</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/4/4d/T--iTesla-SoundBio--Lab_Notebook_2018-8-29.pdf"> | ||
+ | Practice FastClone Transformation</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/08/31</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/6/6c/T--iTesla-SoundBio--Lab_Notebook_2018-8-31.pdf"> | ||
+ | Miniprep and PCR of xylose cassette and PCR of pAX01</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/01</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/d/df/T--iTesla-SoundBio--Lab_Notebook_2018-9-1.pdf"> | ||
+ | more pcrs.....</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/02</p> | ||
+ | <a href= "hhttps://static.igem.org/mediawiki/2018/8/8d/T--iTesla-SoundBio--Lab_Notebook_2018-9-2.pdf"> | ||
+ | Analytical gel of PCRs, digest with DpnI, and transformation</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/08</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/e/e3/T--iTesla-SoundBio--Lab_Notebook_2018-9-8.pdf"> | ||
+ | Transformation of B. subtilis with pAX01 and PCRs of pAX01</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/09</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/e/e8/T--iTesla-SoundBio--Lab_Notebook_2018-9-9.pdf"> | ||
+ | practice transformation with bacillus</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/15</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/9/91/T--iTesla-SoundBio--Lab_Notebook_2018-9-15.pdf"> | ||
+ | Transformation of competent B. subtilis 168</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/21</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/4/40/T--iTesla-SoundBio--Lab_Notebook_2018-9-21.pdf"> | ||
+ | More transformations and minipreps of pAX01</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/22</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/5/5d/T--iTesla-SoundBio--Lab_Notebook_2018-9-22.pdf"> | ||
+ | More transformations with bacillus</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/23 AM</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/4/4d/T--iTesla-SoundBio--Lab_Notebook_2018-9-23-AM.pdf"> | ||
+ | Transformation Tests</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/23 PM</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/5/51/T--iTesla-SoundBio--Lab_Notebook_2018-9-23-PM.pdf.pdf"> | ||
+ | Primer Design</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/28</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/1/18/T--iTesla-SoundBio--Lab_Notebook_2018-9-28.pdf"> | ||
+ | Buffers and Prep stuff</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/29</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/c/c8/T--iTesla-SoundBio--Lab_Notebook_2018-9-29.pdf"> | ||
+ | Colony PCR and transformation stuff</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/09/30</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/1/14/T--iTesla-SoundBio--Lab_Notebook_2018-9-30.pdf"> | ||
+ | More colony PCRs</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/10/03 AM</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/c/ca/T--iTesla-SoundBio--Lab_Notebook_2018-10-03.pdf"> | ||
+ | Instructions for David about colony PCR</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/10/06 PM</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/b/bd/T--iTesla-SoundBio--Lab_Notebook_2018-10-06.pdf"> | ||
+ | analysis of colony pcr results and then...other stuff</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/10/07</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/4/45/T--iTesla-SoundBio--Lab_Notebook_2018-10-07.pdf"> | ||
+ | Leads Meeting</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/10/11</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/3/36/T--iTesla-SoundBio--Lab_Notebook_2018-10-11.pdf"> | ||
+ | Colony PCRs (for integration confirmation and 16s sequencing)</a> | ||
+ | |||
+ | <p><strong>Date:</strong> 2018/10/13</p> | ||
+ | <a href= "https://static.igem.org/mediawiki/2018/1/14/T--iTesla-SoundBio--Lab_Notebook_2018-10-13.pdf"> | ||
+ | Colony PCR for 16s Genome Sequencing</a> | ||
+ | </h2> | ||
+ | </div> |
Revision as of 00:56, 15 October 2018
Recording Our Progress
Date: 2018/06/02
Transform blue coral amilCP into already competent dh5 alpha cellsDate: 2018/06/08
PCR Practice and E. Coli CulturingDate: 2018/06/09
Miniprep Integration vector and BBa_k608006 (biobricking backbone) for future digestion and ligation. Also, to run an analytical gel on last night’s PCR to analyze its success.Date: 2018/06/10
Repeat Miniprep of Biobrick BackboneDate: 2018/06/11
Redo PCR of gblocks and Redo Analytical Gel on First PCRDate: 2018/06/16
Digest of Miniprepped Biobricking Backbone and Analytical GelDate: 2018/06/17
Gel Purifying Digested BB Backbone slices and Digesting PCR'd FC 1Date: 2018/06/21
Second Miniprep of Biobricking Backbone & Integration Vector of B. Subtilis and testing PCRs with phusion stuff AND test-digest for SacII and BglIIDate: 2018/06/22
Gel Test of psb1a3, pax01, and test-digest for BglII/SacII. BB digest of psb1a3, and purification gelDate: 2018/06/30
Gel Extraction of psb1A3, Ligation of FC Frag 1 and psb1a3, and Transformation of Ligated Plasmid into E.coliDate: 2018/07/05
Digesting, running a purification gel and gel extracting miniprepped psb1a3Date: 2018/07/06
Miniprepping psb1a3 (Round #3)Date: 2018/07/07
Digesting and gel purifying psb1a3, PCRing Frag 1Date: 2018/07/12
Gel extracting digested psb1a3, PCRing and PCR purifying Factor C Frag 2Date: 2018/07/14
Digest psb1a3, gel purify, gel extract and nanodrop digested psb1a3Date: 2018/07/16
Digesting Frag 1, Ligating Frag 1 and Digested psb1a3, Transforming into E.coli, Transforming Interlab plasmids into E.coli, and Miniprepping psb1a3Date: 2018/07/20
ESETITTTTTTTTTTTTTTTTTTTTTTTTTDate: 2018/07/21
PCRing more Fragment 1, Running an analytical gel, digesting psb1a3 and psb1c3, running a purificaiton gel, gel extracting and nanodropping resulting digestsDate: 2018/07/22
Miniprepping psb1a3 and psb1c3, digesting, running a purification gel and gel extractingDate: 2018/07/27
Digesting Frag 1, Ligating Frag 1 and psb1a3/c3, Transforming into E.coliDate: 2018/07/29
More PCRs of Fragment 1Date: 2018/07/30
Miniprep, digest, and gel purification of pSB1C3, pcr and pcr purification of fragment 1 geneblockDate: 2018/07/31
Miniprepping psb1a3 (Round #3)Date: 2018/08/01
Running a Purification Gel on Digested FC 1 NOT SUCCESSFULDate: 2018/08/02
Purification Gel of psb1C3Date: 2018/08/03
PCR fragment 1Date: 2018/08/07
PCR, Digest Fragment 1 & Digest psb1C3Date: 2018/08/08
Debugging PCR using psb1C3 and DMSODate: 2018/08/09
PCR'ing Fragment 2, Digesting psb1C3Date: 2018/08/10
Gel extraction of digested fragment 2Date: 2018/08/11
Analytical gel of frag 1 PCRs and gel extraction of fragment 2 digestsDate: 2018/08/12
Ligation of pSB1C3 + frag 2 and transformationDate: 2018/08/21
Miniprep of pSB1C3+mRFP, PCR of fragment 2 and fragment 1Date: 2018/08/22
PCR of fragments 1, 2, and pSB1C3 and analytical gelDate: 2018/08/26
PCR of fragments 1 and 2Date: 2018/08/27
Practice with bacillusDate: 2018/08/29
Practice FastClone TransformationDate: 2018/08/31
Miniprep and PCR of xylose cassette and PCR of pAX01Date: 2018/09/01
more pcrs.....Date: 2018/09/02
Analytical gel of PCRs, digest with DpnI, and transformationDate: 2018/09/08
Transformation of B. subtilis with pAX01 and PCRs of pAX01Date: 2018/09/09
practice transformation with bacillusDate: 2018/09/15
Transformation of competent B. subtilis 168Date: 2018/09/21
More transformations and minipreps of pAX01Date: 2018/09/22
More transformations with bacillusDate: 2018/09/23 AM
Transformation TestsDate: 2018/09/23 PM
Primer DesignDate: 2018/09/28
Buffers and Prep stuffDate: 2018/09/29
Colony PCR and transformation stuffDate: 2018/09/30
More colony PCRsDate: 2018/10/03 AM
Instructions for David about colony PCRDate: 2018/10/06 PM
analysis of colony pcr results and then...other stuffDate: 2018/10/07
Leads MeetingDate: 2018/10/11
Colony PCRs (for integration confirmation and 16s sequencing)Date: 2018/10/13
Colony PCR for 16s Genome Sequencing