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| − | <div class="column full_size judges-will-not-evaluate"> | + | <div class="row"><h1>Part: PRhl_31_CheZ_J23117_34_RhlR</h1></div> |
| − | <h3>★ ALERT! </h3> | + | <div class="row"><h2>Bba_K2594011</h2></div> |
| − | <p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p> | + | <div class="row"><p> The plasmid senses and responds to N-butanoyl-L-Homoserine Lactone (C4-HSL), a quorum sensing molecule that is present in Pseudomonas aeruginosa, a common component of biofilms. The J23117 promoter constitutively expresses RhlR, which activates the PRhl promoter site when bound to C4-HSL. Once quorum sensing signals are detected, the production of CheZ, a protein responsible for chemotactic ability, begins. As the plasmid senses C4-HSL, the microbe begins moving in a straight-line path along the concentration gradient. If the microbe leaves the concentration gradient, it will begin tumbling again until it enters back into the gradient, eventually arriving at the biofilm. </ p></ div> |
| − | <p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p> | + | |
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| − | <h1>Composite Parts</h1>
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| − | A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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| − | <p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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| − | <h3>Note</h3>
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| − | <p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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| − | <h3>Best Composite Part Special Prize</h3>
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| − | <p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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| − | <b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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Part: PRhl_31_CheZ_J23117_34_RhlR
Bba_K2594011
The plasmid senses and responds to N-butanoyl-L-Homoserine Lactone (C4-HSL), a quorum sensing molecule that is present in Pseudomonas aeruginosa, a common component of biofilms. The J23117 promoter constitutively expresses RhlR, which activates the PRhl promoter site when bound to C4-HSL. Once quorum sensing signals are detected, the production of CheZ, a protein responsible for chemotactic ability, begins. As the plasmid senses C4-HSL, the microbe begins moving in a straight-line path along the concentration gradient. If the microbe leaves the concentration gradient, it will begin tumbling again until it enters back into the gradient, eventually arriving at the biofilm.
