Difference between revisions of "Team:Bordeaux/Safety"

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{{Bordeaux}}
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{{:Team:Bordeaux/Template/Navbar}}
 
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<h1> Safety </h1>
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        /* Top Banner */
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        /* Presentation text*/
  
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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            width: 100% !important;
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<li>Choosing a non-pathogenic chassis</li>
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            background-color: #3F51B5;
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<li>Choosing parts that will not harm humans / animals / plants</li>
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            color: white;
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        }
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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</div>
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            background-color: #FF9800;
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            color: white;
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h3>Safe Shipment</h3>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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        <h1 class="text-longshadow">Safety</h1>
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        <p>Safety is our main concern at iGEM Bordeaux. We were committed in order to identify and manage risks involved by
 +
            our project. To reach this goal, we implemented containment practices and good laboratory practices in accordance
 +
            to practices already used in our laboratory, in order to avoid any risks for lab workers and environment.</p>
 +
        <p>Members of the team who have worked in the lab received specific safety training by lab’s staff. This training provided
 +
            basic information such as correct conduct to adopt in case of accident or fire including location of emergency
 +
            exit or safety showers.
 +
        </p>
 +
        <h2>Biologicals risks</h2>
 +
        <p>Our project included no specific biological risks with the use of Escherichia coli strain DH5alpha classified as
 +
            risk group 1 organisms. This strain will not cause any disease issue for healthy adult. The only health issue
 +
            would be for immunosuppressed individual in case of ingestion. Another risk would be the cross contamination
 +
            of common materials or other culture at the lab. Moreover, as we use genetic engineered organism with especially
 +
            antibiotic resistance gene, we need to avoid any environmental exposure to these organisms. In order to prevent
 +
            theses issues, we have implemented strictly containment practices such as the use of biosafety cabinet and specific
 +
            disposal for biological waste In case of development of our project at bigger scale, the containment of our engineered
 +
            E.coli will not be an issue as the biocatalysis of HMF in FDCA could happen in a closed bioreactor.
 +
        </p>
 +
        <h2>Chemical risks</h2>
 +
        <p>The main risks involved by our project will be the use of chemicals. Main risks are caused by the use of EtBr (Ethidium
 +
            bromide) which is carcinogen and HMF which can cause skin, eyes and respiratory irritation. The use of nitrile
 +
            gloves, protection glasses and lab coat are mandatory to prevent any contact. Since HMF is a powder, it is handled
 +
            under a laboratory fume hood to prevent respiratory exposure.
 +
        </p>
 +
        <p>In order to implement an efficient safety policy into iGEM Bordeaux activities, we try to identify and assess every
 +
            risk involved by our project and take measure to prevent any of this risk.</p>
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Revision as of 08:19, 15 October 2018

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Safety

Safety is our main concern at iGEM Bordeaux. We were committed in order to identify and manage risks involved by our project. To reach this goal, we implemented containment practices and good laboratory practices in accordance to practices already used in our laboratory, in order to avoid any risks for lab workers and environment.

Members of the team who have worked in the lab received specific safety training by lab’s staff. This training provided basic information such as correct conduct to adopt in case of accident or fire including location of emergency exit or safety showers.

Biologicals risks

Our project included no specific biological risks with the use of Escherichia coli strain DH5alpha classified as risk group 1 organisms. This strain will not cause any disease issue for healthy adult. The only health issue would be for immunosuppressed individual in case of ingestion. Another risk would be the cross contamination of common materials or other culture at the lab. Moreover, as we use genetic engineered organism with especially antibiotic resistance gene, we need to avoid any environmental exposure to these organisms. In order to prevent theses issues, we have implemented strictly containment practices such as the use of biosafety cabinet and specific disposal for biological waste In case of development of our project at bigger scale, the containment of our engineered E.coli will not be an issue as the biocatalysis of HMF in FDCA could happen in a closed bioreactor.

Chemical risks

The main risks involved by our project will be the use of chemicals. Main risks are caused by the use of EtBr (Ethidium bromide) which is carcinogen and HMF which can cause skin, eyes and respiratory irritation. The use of nitrile gloves, protection glasses and lab coat are mandatory to prevent any contact. Since HMF is a powder, it is handled under a laboratory fume hood to prevent respiratory exposure.

In order to implement an efficient safety policy into iGEM Bordeaux activities, we try to identify and assess every risk involved by our project and take measure to prevent any of this risk.