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Revision as of 08:53, 15 October 2018
Our Mission
Our Project
Our Lab
The parts of our fusion proteins were ordered as separate sequences: mRPF, Prancer Purple, sgBP, silk repeats, cellulose binding module, and keratin binding domain. Constructs containing a chromoprotein sequence, a binding domain, and silk repeats in different combinations were assembled with Golden Gate. Purified and evaluated plasmids were transformed in E. coli production strain BL21, and the constructs were produced by inducing with IPTG. Proteins were purified with ÄKTA or a heat-based protocol based on our modeling results.
The tests performed included:
- The effect of protein production on cell growth.
- The binding of the cellulose binding module to nanocellulose and untreated cotton.
- The binding of the keratin binding domain to a keratin supplement and hair.
- The production of colorful silk fibers.
Our Team
Our Story