Difference between revisions of "Team:Munich/chibiobrick1.html"
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<a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>, | <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>, | ||
<a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Mini prep</a>, | <a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Mini prep</a>, | ||
| − | <a href="https://static.igem.org/mediawiki/2018/a/ab/T--Munich--WL1_Sequencing_sample_preparation.pdf" target="_blank">Sequencing</a> | + | <a href="https://static.igem.org/mediawiki/2018/a/ab/T--Munich--WL1_Sequencing_sample_preparation.pdf" target="_blank">Sequencing</a> |
Revision as of 13:38, 15 October 2018
Forming oligodimers from single-strand DNA chi6 sequences
2018/06/01| Participants: | Dominic |
| Protocol: | Oligodimerization |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic |
| Protocol: | Restrition digest, Ligation, Chemical transformation, Mini prep, Sequencing |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic |
| Protocol: | restriction digest, PCR purification, ligation, chem trafo, PCR, Agarose gel |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination. |