Difference between revisions of "Team:Munich/chibiobrick1.html"
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<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td><a href="https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html" target="_blank">Oligodimerization</a> | + | <td><a href="https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html" target="_blank">Oligodimerization</a> </td> |
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</table> | </table> | ||
Revision as of 14:01, 15 October 2018
Forming oligodimers from single-strand DNA chi6 sequences
2018/06/01| Participants: | Dominic |
| Protocol: | Oligodimerization |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic |
| Protocol: | Restrition digest, Ligation, Chemical transformation, Mini prep, Sequencing |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic |
| Protocol: | Restrition digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination. |