Difference between revisions of "Team:Munich/chiinhibition1.html"
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> | + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, |
RecBCD Inhibition in vitro | RecBCD Inhibition in vitro | ||
</td> | </td> | ||
| Line 35: | Line 35: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> | + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, |
RecBCD Inhibition in vitro | RecBCD Inhibition in vitro | ||
</td> | </td> | ||
| Line 61: | Line 61: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> | + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, |
RecBCD Inhibition in vitro | RecBCD Inhibition in vitro | ||
</tr> | </tr> | ||
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<td> | <td> | ||
<a href="https://static.igem.org/mediawiki/2018/7/7e/T--Munich--WL1_Restriction_Digest.pdf" target="_blank">Restrition digest</a>, | <a href="https://static.igem.org/mediawiki/2018/7/7e/T--Munich--WL1_Restriction_Digest.pdf" target="_blank">Restrition digest</a>, | ||
| − | <a href="https://international.neb.com/protocols/2015/11/23/monarch-pcr-and-dna-cleanup-kit-protocol" target="_blank">PCR purification</a> | + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-pcr-and-dna-cleanup-kit-protocol" target="_blank">PCR purification</a> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 108: | Line 108: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> | + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, |
RecBCD Inhibition in vitro | RecBCD Inhibition in vitro | ||
</tr> | </tr> | ||
Revision as of 14:54, 15 October 2018
In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/06/15| Participants: | Dominic Schwarz |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Results: | linear DNA was not degraded: we found out later that RecBCD was expired, but a positive control was missing. |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/06/19| Participants: | Enikö Baligács |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Notes: | The experiment was redone by another student and a positive control was added. |
| Results: | linear DNA was not degraded: We found out that RecBCD was expired and not functional. |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/07/02| Participants: | Julia Mayer |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Notes: | |
| Results: | The agarose gel was prepared wrong. Therefore, samples were lost and could not be evaluated. |
linearizing pSB1C3_RFP
2018/08/01| Participants: | Dominic Schwarz |
| Protocol: | Restrition digest, PCR purification |
| Notes: | EcoRI-HF |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/08/02| Participants: | Enikö Baligács, Julia Mayer |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Notes: | The experiment was repeated with newly ordered recBCD |
| Results: | Chi6 oligomers inhibit RecBCD but are degraded within 30min. We went on to show these results in cell extract. |
