Difference between revisions of "Team:UMaryland/PETaseIntro"
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SUMMARY OF WET LAB RESULTS | SUMMARY OF WET LAB RESULTS | ||
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UC DAVIS 2012 | UC DAVIS 2012 | ||
| − | Confirmed cutinase activity using PNPB esterase assay | + | <p> |
| − | Engineered E. coli ethylene glycol metabolism with directed evolution | + | <p> |
| + | Confirmed cutinase activity using PNPB esterase assay | ||
| + | <p> | ||
| + | <p> | ||
| + | Engineered E. coli ethylene glycol metabolism with directed evolution | ||
| + | <p> | ||
| + | <p> | ||
BAU-Indonesia 2012 | BAU-Indonesia 2012 | ||
| − | Isolation of cutinase gene from nature with primers | + | <p> |
| + | <p> | ||
| + | Isolation of cutinase gene from nature with primers | ||
| + | <p> | ||
| + | <p> | ||
TU Darmstadt 2012 | TU Darmstadt 2012 | ||
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| + | <p> | ||
Surface display of cutinase on E. coli | Surface display of cutinase on E. coli | ||
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| + | <p> | ||
Attempted TPA transport into E. coli, further research required | Attempted TPA transport into E. coli, further research required | ||
| + | <p> | ||
| + | <p> | ||
Expressed all TPH enzymes, did not attempt to measure activity | Expressed all TPH enzymes, did not attempt to measure activity | ||
| + | <p> | ||
| + | <p> | ||
Confirmed anaerobic conversion of PCA via AroY and XylE enzymes | Confirmed anaerobic conversion of PCA via AroY and XylE enzymes | ||
| + | <p> | ||
| + | <p> | ||
Imperial_College 2013 | Imperial_College 2013 | ||
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Produced P3HB bioplastic from mixed waste containing at least some PET | Produced P3HB bioplastic from mixed waste containing at least some PET | ||
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METU_Turkey 2014 | METU_Turkey 2014 | ||
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Reduced catechol to pyruvate | Reduced catechol to pyruvate | ||
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ITB-Indonesia 2014 | ITB-Indonesia 2014 | ||
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LC cutinatse activity confimed with SEM, PNPB | LC cutinatse activity confimed with SEM, PNPB | ||
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Pasteur Paris 2015 | Pasteur Paris 2015 | ||
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PNPB assay to confirm activity of esterase EST13 | PNPB assay to confirm activity of esterase EST13 | ||
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Fluorescent detection of TPA can not be accomplished when in LB broth. | Fluorescent detection of TPA can not be accomplished when in LB broth. | ||
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Harvard 2016 | Harvard 2016 | ||
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Petase function confirmed with PNPB | Petase function confirmed with PNPB | ||
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Bacteria produced electric current when supplied with unspecified quantity of TPA | Bacteria produced electric current when supplied with unspecified quantity of TPA | ||
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ASIJ Tokyo 2016 | ASIJ Tokyo 2016 | ||
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Attempt at detecting PET degradation by mass change failed | Attempt at detecting PET degradation by mass change failed | ||
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UoA_NewZealand 2016 | UoA_NewZealand 2016 | ||
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Assembled PETase part with His tag | Assembled PETase part with His tag | ||
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BGU-Israel 2016 | BGU-Israel 2016 | ||
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PNPB and EM to confirm LC cutinase activity | PNPB and EM to confirm LC cutinase activity | ||
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P. putida can grow on PCA as sole carbon source, but not TPA. | P. putida can grow on PCA as sole carbon source, but not TPA. | ||
| − | E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation. Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution | + | <p> |
| + | <p> | ||
| + | E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation. | ||
| + | <p> | ||
| + | <p> | ||
| + | Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution | ||
| + | <p> | ||
| + | <p> | ||
Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity. | Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity. | ||
| + | <p> | ||
| + | <p> | ||
TJUSLS China 2016 | TJUSLS China 2016 | ||
| + | <p> | ||
| + | <p> | ||
HPLC detection of MHET to confirm PETase activity in varying conditions | HPLC detection of MHET to confirm PETase activity in varying conditions | ||
| + | <p> | ||
| + | <p> | ||
Surface display of PETase in E. coli | Surface display of PETase in E. coli | ||
| + | <p> | ||
| + | <p> | ||
Tianjin 2016 | Tianjin 2016 | ||
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| + | <p> | ||
EM confirmation of PETase activity of PET film degradation | EM confirmation of PETase activity of PET film degradation | ||
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Multispectral scanning quantified PETase products for cell free system | Multispectral scanning quantified PETase products for cell free system | ||
| + | <p> | ||
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Baltimore BioCrew 2016 | Baltimore BioCrew 2016 | ||
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Planned to weigh PET degradation, no results | Planned to weigh PET degradation, no results | ||
| + | <p> | ||
| + | <p> | ||
UESTC China 2016 | UESTC China 2016 | ||
| + | <p> | ||
| + | <p> | ||
SEM and PNPB to confirm PETase activity | SEM and PNPB to confirm PETase activity | ||
| + | <p> | ||
| + | <p> | ||
Possible detection of TPA by UV vis (higher absorbance across spectrum) | Possible detection of TPA by UV vis (higher absorbance across spectrum) | ||
| + | <p> | ||
| + | <p> | ||
AUC_Turkey 2016 | AUC_Turkey 2016 | ||
| + | <p> | ||
| + | <p> | ||
Withdrawn | Withdrawn | ||
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| + | <p> | ||
ITB-Indonesia 2017 | ITB-Indonesia 2017 | ||
| + | <p> | ||
| + | <p> | ||
PNPB and SEM to confirm PETase activity | PNPB and SEM to confirm PETase activity | ||
| + | <p> | ||
| + | <p> | ||
Successful biofilm formation on PET, but biofilm matrix hampered PETase activity. | Successful biofilm formation on PET, but biofilm matrix hampered PETase activity. | ||
| + | <p> | ||
| + | <p> | ||
Baltimore BioCrew 2017 | Baltimore BioCrew 2017 | ||
| + | <p> | ||
| + | <p> | ||
Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity | Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity | ||
| + | <p> | ||
| + | <p> | ||
BOKU-Vienna 2017 | BOKU-Vienna 2017 | ||
| + | <p> | ||
| + | <p> | ||
Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded | Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded | ||
| − | + | <p> | |
| + | <p> | ||
If you see this then 2018 results from other teams have not been posted in time to show them here before the wiki freeze. | If you see this then 2018 results from other teams have not been posted in time to show them here before the wiki freeze. | ||
<p> | <p> | ||
Revision as of 16:29, 15 October 2018
History
iGEM teams pursuing PET related projects
SUMMARY OF WET LAB RESULTS
UC DAVIS 2012
Confirmed cutinase activity using PNPB esterase assay
Engineered E. coli ethylene glycol metabolism with directed evolution
BAU-Indonesia 2012
Isolation of cutinase gene from nature with primers
TU Darmstadt 2012
Surface display of cutinase on E. coli
Attempted TPA transport into E. coli, further research required
Expressed all TPH enzymes, did not attempt to measure activity
Confirmed anaerobic conversion of PCA via AroY and XylE enzymes
Imperial_College 2013
Produced P3HB bioplastic from mixed waste containing at least some PET
METU_Turkey 2014
Reduced catechol to pyruvate
ITB-Indonesia 2014
LC cutinatse activity confimed with SEM, PNPB
Pasteur Paris 2015
PNPB assay to confirm activity of esterase EST13
Fluorescent detection of TPA can not be accomplished when in LB broth.
Harvard 2016
Petase function confirmed with PNPB
Bacteria produced electric current when supplied with unspecified quantity of TPA
ASIJ Tokyo 2016
Attempt at detecting PET degradation by mass change failed
UoA_NewZealand 2016
Assembled PETase part with His tag
BGU-Israel 2016
PNPB and EM to confirm LC cutinase activity
P. putida can grow on PCA as sole carbon source, but not TPA.
E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation.
Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution
Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity.
TJUSLS China 2016
HPLC detection of MHET to confirm PETase activity in varying conditions
Surface display of PETase in E. coli
Tianjin 2016
EM confirmation of PETase activity of PET film degradation
Multispectral scanning quantified PETase products for cell free system
Baltimore BioCrew 2016
Planned to weigh PET degradation, no results
UESTC China 2016
SEM and PNPB to confirm PETase activity
Possible detection of TPA by UV vis (higher absorbance across spectrum)
AUC_Turkey 2016
Withdrawn
ITB-Indonesia 2017
PNPB and SEM to confirm PETase activity
Successful biofilm formation on PET, but biofilm matrix hampered PETase activity.
Baltimore BioCrew 2017
Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity
BOKU-Vienna 2017
Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded
If you see this then 2018 results from other teams have not been posted in time to show them here before the wiki freeze.