History

iGEM teams pursuing PET related projects

SUMMARY OF WET LAB RESULTS

UC DAVIS 2012

- Confirmed cutinase activity using PNPB esterase assay

- Engineered E. coli ethylene glycol metabolism with directed evolution

BAU-Indonesia 2012

- Isolation of cutinase gene from nature with primers

TU Darmstadt 2012

- Surface display of cutinase on E. coli

- Attempted TPA transport into E. coli, further research required

- Expressed all TPH enzymes, did not attempt to measure activity

- Confirmed anaerobic conversion of PCA via AroY and XylE enzymes

Imperial_College 2013

- Produced P3HB bioplastic from mixed waste containing at least some PET

METU_Turkey 2014

- Reduced catechol to pyruvate

ITB-Indonesia 2014

- LC cutinatse activity confimed with SEM, PNPB

Pasteur Paris 2015

- PNPB assay to confirm activity of esterase EST13

- Fluorescent detection of TPA can not be accomplished when in LB broth.

Harvard 2016

- Petase function confirmed with PNPB

- Bacteria produced electric current when supplied with unspecified quantity of TPA

ASIJ Tokyo 2016

- Attempt at detecting PET degradation by mass change failed

UoA_NewZealand 2016

- Assembled PETase part with His tag

BGU-Israel 2016

- PNPB and EM to confirm LC cutinase activity

- P. putida can grow on PCA as sole carbon source, but not TPA.

- E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation.

- Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution

- Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity.

TJUSLS China 2016

- HPLC detection of MHET to confirm PETase activity in varying conditions

- Surface display of PETase in E. coli

Tianjin 2016

- EM confirmation of PETase activity of PET film degradation

- Multispectral scanning quantified PETase products for cell free system

Baltimore BioCrew 2016

- Planned to weigh PET degradation, no results

UESTC China 2016

- SEM and PNPB to confirm PETase activity

- Possible detection of TPA by UV vis (higher absorbance across spectrum)

AUC_Turkey 2016

- Withdrawn

ITB-Indonesia 2017

- PNPB and SEM to confirm PETase activity

- Successful biofilm formation on PET, but biofilm matrix hampered PETase activity.

Baltimore BioCrew 2017

- Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity

BOKU-Vienna 2017

- Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded

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If you see this then 2018 results from other teams have not been posted in time to show them here before the wiki freeze.