Difference between revisions of "Team:UMaryland/PETaseIntro"
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- Engineered E. coli ethylene glycol metabolism with directed evolution | - Engineered E. coli ethylene glycol metabolism with directed evolution | ||
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BAU-Indonesia 2012 | BAU-Indonesia 2012 | ||
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- Isolation of cutinase gene from nature with primers | - Isolation of cutinase gene from nature with primers | ||
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TU Darmstadt 2012 | TU Darmstadt 2012 | ||
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- Confirmed anaerobic conversion of PCA via AroY and XylE enzymes | - Confirmed anaerobic conversion of PCA via AroY and XylE enzymes | ||
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Imperial_College 2013 | Imperial_College 2013 | ||
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- Produced P3HB bioplastic from mixed waste containing at least some PET | - Produced P3HB bioplastic from mixed waste containing at least some PET | ||
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METU_Turkey 2014 | METU_Turkey 2014 | ||
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- Reduced catechol to pyruvate | - Reduced catechol to pyruvate | ||
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ITB-Indonesia 2014 | ITB-Indonesia 2014 | ||
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- LC cutinatse activity confimed with SEM, PNPB | - LC cutinatse activity confimed with SEM, PNPB | ||
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Pasteur Paris 2015 | Pasteur Paris 2015 | ||
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- Fluorescent detection of TPA can not be accomplished when in LB broth. | - Fluorescent detection of TPA can not be accomplished when in LB broth. | ||
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Harvard 2016 | Harvard 2016 | ||
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- Bacteria produced electric current when supplied with unspecified quantity of TPA | - Bacteria produced electric current when supplied with unspecified quantity of TPA | ||
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ASIJ Tokyo 2016 | ASIJ Tokyo 2016 | ||
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- Attempt at detecting PET degradation by mass change failed | - Attempt at detecting PET degradation by mass change failed | ||
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UoA_NewZealand 2016 | UoA_NewZealand 2016 | ||
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- Assembled PETase part with His tag | - Assembled PETase part with His tag | ||
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BGU-Israel 2016 | BGU-Israel 2016 | ||
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- Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity. | - Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity. | ||
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TJUSLS China 2016 | TJUSLS China 2016 | ||
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- Surface display of PETase in E. coli | - Surface display of PETase in E. coli | ||
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Tianjin 2016 | Tianjin 2016 | ||
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- Multispectral scanning quantified PETase products for cell free system | - Multispectral scanning quantified PETase products for cell free system | ||
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Baltimore BioCrew 2016 | Baltimore BioCrew 2016 | ||
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- Planned to weigh PET degradation, no results | - Planned to weigh PET degradation, no results | ||
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UESTC China 2016 | UESTC China 2016 | ||
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- Possible detection of TPA by UV vis (higher absorbance across spectrum) | - Possible detection of TPA by UV vis (higher absorbance across spectrum) | ||
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AUC_Turkey 2016 | AUC_Turkey 2016 | ||
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- Withdrawn | - Withdrawn | ||
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ITB-Indonesia 2017 | ITB-Indonesia 2017 | ||
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- Successful biofilm formation on PET, but biofilm matrix hampered PETase activity. | - Successful biofilm formation on PET, but biofilm matrix hampered PETase activity. | ||
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Baltimore BioCrew 2017 | Baltimore BioCrew 2017 | ||
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- Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity | - Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity | ||
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BOKU-Vienna 2017 | BOKU-Vienna 2017 | ||
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Revision as of 16:41, 15 October 2018
History
iGEM teams pursuing PET related projects
SUMMARY OF WET LAB RESULTS
UC DAVIS 2012
- Confirmed cutinase activity using PNPB esterase assay
- Engineered E. coli ethylene glycol metabolism with directed evolution
BAU-Indonesia 2012
- Isolation of cutinase gene from nature with primers
TU Darmstadt 2012
- Surface display of cutinase on E. coli
- Attempted TPA transport into E. coli, further research required
- Expressed all TPH enzymes, did not attempt to measure activity
- Confirmed anaerobic conversion of PCA via AroY and XylE enzymes
Imperial_College 2013
- Produced P3HB bioplastic from mixed waste containing at least some PET
METU_Turkey 2014
- Reduced catechol to pyruvate
ITB-Indonesia 2014
- LC cutinatse activity confimed with SEM, PNPB
Pasteur Paris 2015
- PNPB assay to confirm activity of esterase EST13
- Fluorescent detection of TPA can not be accomplished when in LB broth.
Harvard 2016
- Petase function confirmed with PNPB
- Bacteria produced electric current when supplied with unspecified quantity of TPA
ASIJ Tokyo 2016
- Attempt at detecting PET degradation by mass change failed
UoA_NewZealand 2016
- Assembled PETase part with His tag
BGU-Israel 2016
- PNPB and EM to confirm LC cutinase activity
- P. putida can grow on PCA as sole carbon source, but not TPA.
- E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation.
- Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution
- Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity.
TJUSLS China 2016
- HPLC detection of MHET to confirm PETase activity in varying conditions
- Surface display of PETase in E. coli
Tianjin 2016
- EM confirmation of PETase activity of PET film degradation
- Multispectral scanning quantified PETase products for cell free system
Baltimore BioCrew 2016
- Planned to weigh PET degradation, no results
UESTC China 2016
- SEM and PNPB to confirm PETase activity
- Possible detection of TPA by UV vis (higher absorbance across spectrum)
AUC_Turkey 2016
- Withdrawn
ITB-Indonesia 2017
- PNPB and SEM to confirm PETase activity
- Successful biofilm formation on PET, but biofilm matrix hampered PETase activity.
Baltimore BioCrew 2017
- Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity
BOKU-Vienna 2017
- Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded
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If you see this then 2018 results from other teams have not been posted in time to show them here before the wiki freeze.