Difference between revisions of "Team:Munich/assembly2.html"
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<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Brigit</td> | + | <td>Brigit Tunaj</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 31: | Line 33: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Brigit</td> | + | <td>Brigit Tunaj</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 54: | Line 58: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Sophie | + | <td>Sophie Kurzbach</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 77: | Line 84: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Brigit</td> | + | <td>Brigit Tunaj</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 101: | Line 111: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Sophie | + | <td>Sophie Kurzbach</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> Burst size analysis, | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/0/03/T--Munich--BurstSizeAnalysisHeinemann2007.pdf" target="_blank">Burst size analysis</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 123: | Line 137: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Sophie | + | <td>Sophie Kurzbach</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 145: | Line 162: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> Brigit</td> | + | <td> Brigit Tunaj</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 168: | Line 188: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Sophie | + | <td>Sophie Kurzbach</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 192: | Line 215: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> Brigit</td> | + | <td> Brigit Tunaj</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 215: | Line 241: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> Brigit</td> | + | <td> Brigit Tunaj</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 236: | Line 265: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Sophie | + | <td>Sophie Kurzbach</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> Burst size analysis, | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/0/03/T--Munich--BurstSizeAnalysisHeinemann2007.pdf" target="_blank">Burst size analysis</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 19:46, 15 October 2018
T7 and Phage Assembly in commercial cell-extract
2018/07/31| Participants: | Brigit Tunaj |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | T7 and T4 Phage: 2% PEG, 4% PEG, 6% PEG |
| Results: | T4 4%: 3*10^4 PFU/mL, T7 %, 1*10^8 PFU/mL |
Phage Titer from T4 and T7
2018/08/02| Participants: | Brigit Tunaj |
| Protocol: | Agar overlay plaque assay |
| Notes: | - |
| Results: | T4 Phage: 1,5*10^ 10 PFU/mL T7 Phage: 5*10^9 PFU/mL |
T7 Phage Assembly in commercial cell-extract
2018/08/08- 2018/08/09| Participants: | Sophie Kurzbach |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | DNA from Kilian (10,37 nM) |
| Results: | no plaques |
T4 and 3S Phage Assembly in commercial cell-extract
2018/08/08- 2018/08/09| Participants: | Brigit Tunaj |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | - |
| Results: | 3S Phage: 2*10^3 PFU/mL T4 Phage: 5*10^4 PFU/mL |
T7 Phage Burst size Analysis and Assembly in commercial cell-extract
2018/08/08| Participants: | Sophie Kurzbach |
| Protocol: | Burst size analysis, PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | - |
| Results: | Burst size: 408 |
T7 Phage Assembly in commercial cell-extract
2018/08/10- 2018/08/11| Participants: | Sophie Kurzbach |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | DNA from Kilian (10,37 nM) |
| Results: | no plaques |
3S Phage Assembly in commercial cell-extract
2018/08/07 2018/08/08| Participants: | Brigit Tunaj |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | - |
| Results: | no plaques |
T7 Phage Assembly in commercial cell-extract
2018/08/13- 2018/08/14| Participants: | Sophie Kurzbach |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | DNA from Kilian |
| Results: | no plaques |
T4 and 3S Phage Assembly in commercial cell-extract
2018/08/16- 2018/08/17| Participants: | Brigit Tunaj |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | DNA:300 ng |
| Results: | T4 Phage: 1*10^3 PFU/mL T7 Phage: 2*10^3 PFU/mL |
| Participants: | Brigit Tunaj |
| Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | - |
| Results: | 10^3 PFU/mL |
| Participants: | Sophie Kurzbach |
| Protocol: | Burst size analysis, PhageT7AssemblyTXTL, Agar overlay plaque assay |
| Notes: | - |
| Results: | Burst size: 1250 |