Difference between revisions of "Team:Munich/engineering4.html"
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<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
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<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Mini Prep</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 20:50, 15 October 2018
9/5
Transforming E. Coli Rosetta for pRED/ET engineering
2018/09/05| Participants: | Dominic Schwarz |
| Protocol: | Electrocompetent transformation |
| Notes: | we used 1µg of DNA for maximum efficiency |
| Results: | No colonies. Something prevents E.Coli Rosetta from being transformed with pRED/ET. Ori's have been checked for compatibility and E.Coli Rosetta can be transformed with plasmids otherwise. We switched focus from this part of the project which is why we did not continue on those results. |
DNA preparation for pRED/ET Engineering
2018/09/05| Participants: | Dominic Schwarz |
| Protocol: | Mini Prep |
| Notes: | |
| Results: | 48ng/ul; This sample was prepared for storage in the Simmel Lab. |