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Revision as of 21:19, 15 October 2018
Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Chemical transformation |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab. |
Redo: Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | Chemical Transformation |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. We decided to redo the experiment by electroporation. |
Redo:Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | Restrition digest |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. pRED/ET engineering seems to not work in Rosetta. we postponed this experiment to do it with PD Dr. Lassak at the LMU again. |