Difference between revisions of "Team:Jiangnan"

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{{Jiangnan}}
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<title>JiangNan</title>
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<h1> Welcome to iGEM 2018! </h1>
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<script type="text/javascript" src="js/main.js"></script>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/materialize/0.100.2/css/materialize.min.css">
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<link rel="stylesheet" type="text/css" href="css/navbar.css">
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<img src="http://placehold.it/1080x320/c4baba/e4dede">
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<ul>
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<a href="#" id="0"><img id="0img" src="./normnavbar/logo.png" style="height: 45px;"></a>
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<ul id="nav-mobile" class="right hide-on-med-and-down">
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<a id="1" class="dropdown-button" href="" data-activates="projectdropdown">
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<img id="1img" src="./normnavbar/project.png">
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<ul id="projectdropdown" class="dropdown-content">
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<li><a href="#">Overview</a></li>
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<li class="divider"></li>
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<li><a href="#">Description</a></li>
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<li class="divider"></li>
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<li><a href="#">achivement</a></li>
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<li class="divider"></li>
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<li><a href="#">design</a></li>
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<li><a href="#">basic parts</a></li>
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<li><a href="#">composite parts</a></li>
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<li><a href="#">protocols</a></li>
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<li><a href="#">interlab</a></li>
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<li><a href="#">lab journal</a></li>
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<li><a href="#">about us</a></li>
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<li><a href="#">attribution</a></li>
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<a id="7" class="dropdown-button" href="safety.html" data-activates="safetydropdown">
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h5>Project Description</h5>
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<p>The goal of our project is to enable 3 features of our chassis cell, i.e.</p>
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<p>high titration, suspension cultivation, and broad spectrum to reduce the production cost and increase the yield of viruses for vaccine production.</p>
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<p>The chassis cell we used here is. MDBK cells.</p>
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<p style="width: 700px;">Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titration of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titration. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titration was further increased in response to plasma irradiation. </p>
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<p style="width: 700px;">Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation. </p>
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<img src="mainimages/broadtitle.png" width="300">
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<p style="width: 700px;">Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.  
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<img id="broadmove" src="mainimages/broadmove.png" width="400" style="position: absolute; top: 3000px; right: 40px;">
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      <ul class="section table-of-contents">
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        <li><a href="#high_titer">High titration</a></li>
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        <li><a href="#suspension">Suspension</a></li>
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        <li><a href="#broadspectrum">Broad spectrum</a></li>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Revision as of 10:52, 25 June 2018

<!DOCTYPE html> JiangNan

Project Description

The goal of our project is to enable 3 features of our chassis cell, i.e.

high titration, suspension cultivation, and broad spectrum to reduce the production cost and increase the yield of viruses for vaccine production.

The chassis cell we used here is. MDBK cells.



Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titration of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titration. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titration was further increased in response to plasma irradiation.



Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.



Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.