Difference between revisions of "Team:HZAU-China/Attributions"

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     <link href="bootstrap.min.css" rel="stylesheet">
 
     <!-- 导航栏CSS -->
 
     <!-- 导航栏CSS -->
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             <a href="https://2018.igem.org/Team:HZAU-China"><img src="https://static.igem.org/mediawiki/2018/7/75/T--HZAU-China--logo-nav.png"
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                    <a class="nav_head" href="https://2018.igem.org/Team:HZAU-China">
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                        <span>Project</span>
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                        <span class="xsjPic"></span>
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            margin: 0 !important;
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                    </a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Description">Description</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Design">Design</a>
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        #content {
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Results">Results</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Demonstrate">Demonstrate</a>
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                    <a class="nav_head" href="#">
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                        <span>Wetlab</span>
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                        <span class="xjtPic"></span>
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        #bodyContent h1,
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                    </a>
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        #bodyContent h2,
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
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        #bodyContent h3,
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
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        #bodyContent h4,
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">InterLab</a>
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        #bodyContent h5 {
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                </li>
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        #team_name {
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                        <span>Drylab</span>
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                        <span class="xjtPic"></span>
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                    </a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Model">Model</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Software">Software</a>
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                        <span class="xjtPic"></span>
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                    </a>
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        * {
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
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                        <span>About Us</span>
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                    </a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Team">Team</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Attributions">Attributions</a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Collaborations">Collaborations</a>
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                        <span>Parts</span>
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                 <div class="h2">Wet Lab</div>
 
                 <div class="h2">Wet Lab</div>
 
                 <div class="h3">Gene Editing</div>
 
                 <div class="h3">Gene Editing</div>
−
                 <p><i>SifA</i> knock out is completed by Zhunjun Xia. Zhuoqi Huang and Wenxin Hu also gave
+
                 <p>SifA and SipD knock out is completed by Zhunjun Xia, Zhuoqi Huang and Wenxin Hu also gave
 
                     assistance.</p>
 
                     assistance.</p>
 
                 <div class="h3">Plasmid Construction</div>
 
                 <div class="h3">Plasmid Construction</div>
 
                 <p>Intracellular Environment-Dependent Circuits:</p>
 
                 <p>Intracellular Environment-Dependent Circuits:</p>
−
                 <p>The intracellular environment-dependent circuits is designed by Mo Qiqin and Zhujun Xia.</p>
+
                 <p>The intracellular environment-dependent circuits is designed by Mo Quoin and Zhujun Xia.</p>
 
                 <p>The intracellular environment-dependent circuits is constructed by Mo Qiqin. Ruonan Tian also gave
 
                 <p>The intracellular environment-dependent circuits is constructed by Mo Qiqin. Ruonan Tian also gave
 
                     assistance.</p>
 
                     assistance.</p>
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                 <br>
 
                 <br>
 
                 <p>Targeting Specificity and Surface-Displaying:</p>
 
                 <p>Targeting Specificity and Surface-Displaying:</p>
−
                 <p>RGD-inserted OmpA circuit clone and construction of <i>Salmonella</i> SL1344_RS05255 (OmpA coding
+
                 <p>RGD-inserted OmpA circuit clone and construction of Salmonella SL1344_RS05255 (OmpA coding
 
                     sequence) are completed by Yinqing Zeng and Lingyu Zhong.</p>
 
                     sequence) are completed by Yinqing Zeng and Lingyu Zhong.</p>
 
                 <p>The circuit of surface-display system is constructed by Lingyu Zhong.
 
                 <p>The circuit of surface-display system is constructed by Lingyu Zhong.
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                 <p>Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and three GSDMD full length mutations)
 
                 <p>Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and three GSDMD full length mutations)
 
                     are constructed by Zhiqing Guo and Wenxin Hu.</p>
 
                     are constructed by Zhiqing Guo and Wenxin Hu.</p>
−
                 <p>The description of submitted plasmids by ATP assay and cell phenotype via transfection is designed
+
                 <p>The description of submitted plasmids by ATP assay and cell phenotype via transfection is de-signed
 
                     and completed by Zhujun Xia.
 
                     and completed by Zhujun Xia.
 
                 </p>
 
                 </p>
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                     and CFU measurement of chemical control system.</p>
 
                     and CFU measurement of chemical control system.</p>
 
                 <div class="h3">Cell Phenotype Verification</div>
 
                 <div class="h3">Cell Phenotype Verification</div>
−
                 <p>Cell phenotype verifications are designed and completed by Zhujun Xia, including the verification
+
                 <p>Cell phenotype verifications are designed and completed by Zhujun Xia, including the verifi-cation
 
                     of chemical control system, and targeting specificity, all these circuits and systems work so well.</p>
 
                     of chemical control system, and targeting specificity, all these circuits and systems work so well.</p>
 
                 <div class="h3">Bio-Safety Verification</div>
 
                 <div class="h3">Bio-Safety Verification</div>
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                     our data passed the check of the Measurement Committee successfully.</p>
 
                     our data passed the check of the Measurement Committee successfully.</p>
 
                 <div class="h2">Dry Lab</div>
 
                 <div class="h2">Dry Lab</div>
−
                 <p>Mathematical model and app of <i>Salmonella</i> infection and the releasing of GSDMD are built by Songtao
+
                 <p>Mathematical model and app of salmonella infection and the releasing of GSDMD are built by Songtao
−
                     Chen.</p>
+
                     Chen.
−
                     <p>ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD
+
                     ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD
−
                     is built by Xichen Rao.</p>
+
                     is built by Xichen Rao.
−
                     <p>The modeling of adjusting the strength of RBS and ATc with fixed GSDMD-N275 concentration is completed
+
                     The modeling of adjusting the strength of RBS and ATc with fixed GSDMD-N concentration is completed
−
                     by Yini Miao. The future work is confirming the threshold of GSDMD-N275.</p>
+
                     by Yini Miao. The future work is confirming the threshold of GSDMD-N275.
−
                     <p>The modeling of the promoter sifA and promoter entC is completed by Ruonan Tian.
+
                     The modeling of the promoter sifA and promoter entC is completed by Ruonan Tian.
 
                 </p>
 
                 </p>
 
                 <div class="h2">Software</div>
 
                 <div class="h2">Software</div>
Line 776: Line 565:
 
                 <div class="h2">Cell lines</div>
 
                 <div class="h2">Cell lines</div>
 
                 <p>
 
                 <p>
−
                     Thanks to Prof. Shan Li's laboratory, Huazhong Agricultural University, for sharing the cell lines
+
                     Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the cell lines
 
                     of HEK293T, HELA, MCF7, iBMDM, our experiment can process successfully.
 
                     of HEK293T, HELA, MCF7, iBMDM, our experiment can process successfully.
 
                 </p>
 
                 </p>
−
                 <p> Thanks to Dr. Feng Shao's laboratory, for sharing the cell line of GSDMD<sup>-/-</sup> HELA cells, it provides
+
                 <p> Thanks to Dr. Feng Shao’s laboratory, for sharing the cell line of GSDMD-/- HELA cells, it provides
 
                     us great support.
 
                     us great support.
 
                 </p>
 
                 </p>
Line 786: Line 575:
 
                 </p>
 
                 </p>
 
                 <div class="h2">Bacteria</div>
 
                 <div class="h2">Bacteria</div>
−
                 <p>Thanks to Prof. Shan Li's laboratory, Huazhong Agricultural University, for sharing the strain of
+
                 <p>Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the strain of
−
                     <i>Salmonella</i> <i>enterica</i> var. Typhimurium SL1344.
+
                     Salmonella enterica var. Typhimurium SL1344.
 
                 </p>
 
                 </p>
−
                 <p> Thanks to Dr. Chenli Liu from SIAT CSynBER's generous share (Strain: <i>E.coli</i> K-12 MG1655 with
+
                 <p> Thanks to Dr. Chenli Liu from SIAT CSynBER’s generous share (Strain: E.coli K-12 MG1655 with
 
                     mCherry in the genome), our experiment can process successfully.
 
                     mCherry in the genome), our experiment can process successfully.
 
                 </p>
 
                 </p>
 
                 <div class="h2">Gene Knock In and Knock Out</div>
 
                 <div class="h2">Gene Knock In and Knock Out</div>
−
                 <p>Thanks to Prof. Shan Li's laboratory, Huazhong Agricultural University, for providing the protocol
+
                 <p>Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for providing the protocol
 
                     and material of gene editing based on two-step allelic exchange, it provides us great help.
 
                     and material of gene editing based on two-step allelic exchange, it provides us great help.
 
                 </p>
 
                 </p>
Line 800: Line 589:
 
                 </p>
 
                 </p>
 
                 <div class="h2">Plasmid Backbone</div>
 
                 <div class="h2">Plasmid Backbone</div>
−
                 <p>Thanks to Prof. Shan Li's laboratory, Huazhong Agricultural University, for sharing the plasmid
+
                 <p>Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the plasmid
 
                     backbone (Pcs2-eGFP) to us.</p>
 
                     backbone (Pcs2-eGFP) to us.</p>
 
                 <div class="h2">Sponsors</div>
 
                 <div class="h2">Sponsors</div>
−
                 <p>Thanks to Genescript and IDT for DNA sequence synthesis service.</p>
+
                 <p>Thanks to Genescript and IDT for DNA sequence synthesis service</p>
−
                 <p>Thanks to Snapgene and MathWorks for free software supporting.</p>
+
                 <p>Thanks to Snapgene and MathWorks for free software supporting</p>
 
                 <br>
 
                 <br>
 
                 <br>
 
                 <br>

Revision as of 23:48, 15 October 2018

Attributions

Our project is designed by Zhujun Xia and all circuits is designed by Zhujun Xia, YinQing Zeng and Mo Qiqin.

Wet Lab
Gene Editing

SifA and SipD knock out is completed by Zhunjun Xia, Zhuoqi Huang and Wenxin Hu also gave assistance.

Plasmid Construction

Intracellular Environment-Dependent Circuits:

The intracellular environment-dependent circuits is designed by Mo Quoin and Zhujun Xia.

The intracellular environment-dependent circuits is constructed by Mo Qiqin. Ruonan Tian also gave assistance.


Chemical Control System:

The ATc induced chemical control system is constructed by He Yang, Zhuoqi Huang also gave assistance.

The TetR-eGFP fusion protein expression system for TetR expression measuring is constructed by He Yang, Zhuoqi Huang also gave assistance.


Targeting Specificity and Surface-Displaying:

RGD-inserted OmpA circuit clone and construction of Salmonella SL1344_RS05255 (OmpA coding sequence) are completed by Yinqing Zeng and Lingyu Zhong.

The circuit of surface-display system is constructed by Lingyu Zhong. Optimation of OmpA in E. Coli is completed by Zhujun Xia.


Plasmid Standardization:

Plasmid standardization is completed by Zhiqing Guo, Zhuoqi Huang by using 3A Assembly.


Plasmid Submission:

Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and three GSDMD full length mutations) are constructed by Zhiqing Guo and Wenxin Hu.

The description of submitted plasmids by ATP assay and cell phenotype via transfection is de-signed and completed by Zhujun Xia.

Bacterial Phenotype Verification

Bacterial phenotype verification are designed and completed by He Yang, including the OD measurement and CFU measurement of chemical control system.

Cell Phenotype Verification

Cell phenotype verifications are designed and completed by Zhujun Xia, including the verifi-cation of chemical control system, and targeting specificity, all these circuits and systems work so well.

Bio-Safety Verification

Safety verification is designed and completed by Zhujun Xia, which demonstrated our project is safe enough to be used.

InterLab

InterLab is conducted by Xichen Rao, Zhunjun Xia, Ruonan Tian and Heng Heng also gave assistance, our data passed the check of the Measurement Committee successfully.

Dry Lab

Mathematical model and app of salmonella infection and the releasing of GSDMD are built by Songtao Chen. ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD is built by Xichen Rao. The modeling of adjusting the strength of RBS and ATc with fixed GSDMD-N concentration is completed by Yini Miao. The future work is confirming the threshold of GSDMD-N275. The modeling of the promoter sifA and promoter entC is completed by Ruonan Tian.

Software

The applet of “Bacterial Colony Counter” is designed and programmed by Shuguang Wang.

Human Practice

Collaboration and communication with other teams is completed by Yinqing Zeng. Handicraft manufacture is designed and completed by Yinqing Zeng. Questionnaire preparation, the advertisement of synthetic biology and popular science articles written is completed by Heng Heng. Two posters for Eurasian exchange meetings and CCIC is completed by Ruonan Tian.

Website

Our intergrative wiki is designed and programmed by Shuguang Wang and the artist is designed by Yuying Xiang.

Our blog is built by Shuguang Wang.

Others

The plate reader machine operation is conducted by Mo Qiqin. Chief cleaner of the laboratory is Xichen Rao.

Acknowledgement

For each parts of experiments, our appreciations are as follow:

Cell lines

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the cell lines of HEK293T, HELA, MCF7, iBMDM, our experiment can process successfully.

Thanks to Dr. Feng Shao’s laboratory, for sharing the cell line of GSDMD-/- HELA cells, it provides us great support.

Thanks to Dr. Yi Zeng and Dr. Ms Hong Fan, Shanghai Institute of Biochemistry and Cell Biology, CAS, for sharing the cell line of MDA-MB-231.

Bacteria

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the strain of Salmonella enterica var. Typhimurium SL1344.

Thanks to Dr. Chenli Liu from SIAT CSynBER’s generous share (Strain: E.coli K-12 MG1655 with mCherry in the genome), our experiment can process successfully.

Gene Knock In and Knock Out

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for providing the protocol and material of gene editing based on two-step allelic exchange, it provides us great help.

Thanks to Pan Chu, team leader of iGEM-2016, Huazhong Agricultural University, for teaching us multigene editing based on CRISPR and λ-RED.

Plasmid Backbone

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the plasmid backbone (Pcs2-eGFP) to us.

Sponsors

Thanks to Genescript and IDT for DNA sequence synthesis service

Thanks to Snapgene and MathWorks for free software supporting




Thanks to College of Life Science and technology of Huazhong Agricultural University for providing the site and fund for us.

Thanks to Prof. Binguang Ma for instructing our modeling.

Thanks to Prof. Jin He as our Secondary PI and Prof. Shan Li for instructing our experiment.

Thanks to Prof. Jin He, Prof. Shan Li, Prof. Gang Cao, Prof. Donghai Peng and Prof. Ming Sun for giving us advices at the proposal presentation.

Thanks to Kening Chen, Wenqi Huang and Haimeng Li from iGEM-2017, Huazhong Agricultural University, for giving us advices.

We really appreciate your support!

Team
Attributions

Attributions

Acknowledgement

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