Difference between revisions of "Team:SBS SH 112144/Composite Part"

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<h1>Composite Part</h1>
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<h4> Composite Tagged Lysozyme Gene</h4>
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<img src="https://static.igem.org/mediawiki/2018/4/4e/T--SBS_SH_112144--fused_tagged_lysozyme.png" width=1000 height=400>
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<p>Description: Our composite part within prefix and suffix consists of the most commonly used promoter, RBS, 6x His tag and terminator, as well as our functional coding sequence --- cyanophage lysozyme gene. This composite part is assembled through Gibson Assembly; then, it is fused with linearized vector, pSB1C3 backbone, via infusion. Ultimately, we successfully extract plasmids which contain our target gene from transformed bacteria. Furthermore, we purposely add 6x histag into our sequence in order to improve the efficacy and efficiency of expression and purification; it will also strengthen effects of binding to Ni-NTA column, making it possible to immobilize proteins and lyse cyanobacteria repeatedly in our prototype device. </p>
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<p>Length: 773 bp</p>
  
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<h4> Composite Lysozyme Gene</h4>
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<img src="https://static.igem.org/mediawiki/2018/e/ef/T--SBS_SH_112144--fused_lysozyme.png" width=1000 height=400>
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<h3>★  ALERT! </h3>
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<p>Description: The second part is utilized to demonstrate the pure effects of lysozyme in our function test--- cyanobacterial lysis reaction. Mainly applied in function test, this composite part without 6x his tag was fused with pSB1C3 backbone and then was successfully expressed in E.coli. The lysis reaction was then conducted in the solution with both cultivated cyanobacteria and expressed proteins. In this way, we could examine and quantify the lysis results, confirming the efficacy of cyanophage lysozyme. </p>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p>Length: 747 bp</p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Composite Parts</h1>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Note</h3>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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Revision as of 00:25, 16 October 2018

Composite Part

Composite Tagged Lysozyme Gene

Description: Our composite part within prefix and suffix consists of the most commonly used promoter, RBS, 6x His tag and terminator, as well as our functional coding sequence --- cyanophage lysozyme gene. This composite part is assembled through Gibson Assembly; then, it is fused with linearized vector, pSB1C3 backbone, via infusion. Ultimately, we successfully extract plasmids which contain our target gene from transformed bacteria. Furthermore, we purposely add 6x histag into our sequence in order to improve the efficacy and efficiency of expression and purification; it will also strengthen effects of binding to Ni-NTA column, making it possible to immobilize proteins and lyse cyanobacteria repeatedly in our prototype device.

Length: 773 bp

Composite Lysozyme Gene

Description: The second part is utilized to demonstrate the pure effects of lysozyme in our function test--- cyanobacterial lysis reaction. Mainly applied in function test, this composite part without 6x his tag was fused with pSB1C3 backbone and then was successfully expressed in E.coli. The lysis reaction was then conducted in the solution with both cultivated cyanobacteria and expressed proteins. In this way, we could examine and quantify the lysis results, confirming the efficacy of cyanophage lysozyme.

Length: 747 bp