Difference between revisions of "Team:TPHS San Diego/Results"

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                     This experiment has two parts: one tested the effectiveness of chitinase breakdown using our synthesized strain of ChiA and the other tested the ability of said strain tobe mutated and we used mathematical models to predict whether or not enzyme evolution via error prone PCR is possible.
 
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In proving the ability of GST-ChiA-FLAG (our synthesized construct) to secrete chitinase, we used two methods. In the solution assay there was significant chitinolytic activity and the levels of degradation were measured using colorimetric methods and absorbance measurements. (see Figure 1)
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                        <td><img src = "https://static.igem.org/mediawiki/2018/3/3f/T--TPHS_San_Diego--Fig1.png"></td>
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Revision as of 01:36, 16 October 2018

TPHS IGEM Wiki

Results

Results

This experiment has two parts: one tested the effectiveness of chitinase breakdown using our synthesized strain of ChiA and the other tested the ability of said strain tobe mutated and we used mathematical models to predict whether or not enzyme evolution via error prone PCR is possible.

In proving the ability of GST-ChiA-FLAG (our synthesized construct) to secrete chitinase, we used two methods. In the solution assay there was significant chitinolytic activity and the levels of degradation were measured using colorimetric methods and absorbance measurements. (see Figure 1)

Caption: this is a banana!

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