Difference between revisions of "Team:NUS Singapore-Sci/Parts"

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   <caption style="font-size:13px;"> <strong> Table 1. Basic BioBrik Parts </strong> </caption>
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     <th>Part Name</th>
 
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   <caption style="font-size:13px;"> <strong> Table 2. Composite BioBrik Parts </strong> </caption>
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     <th>Part Name</th>
 
     <th>Part Name</th>

Revision as of 05:54, 16 October 2018

NUS Singapore Science: About Us!

Parts

Basic Parts
Table 1. Basic BioBrick Parts
Part Name Component Description
BBa_K2807000 rAPOBEC1 Rat APOBEC1. Used to perform the C-to-U conversion.
BBa_K2807001 dPspCas13b "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands.
BBa_K2807002 PspCas13b Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands.
BBa_K2807005 eGFP Consistutively expressed GFP reporter. Used in the Reporter system.
BBa_K2807006 mCherry Red flurophore protein. Used in the Reporter system.
BBa_K2807007 T2A Self cleavage peptide. Used in the Reporter system.
BBa_K2807008 GLB1 Human lysosomal acid beta galactosidase. Used in the GLB reporter system.
BBa_K2807009 eGFP mutant reporter eGFP with a mutated start codon (ATG -> ACG).
BBa_K2807010 GLB1 CCG mutant Non-functional beta galactosidase with mutation of I389 to P389.
BBa_K2807011 GLB1 TCT mutant Non-functional beta galactosidase with mutation of F107 to L107.
Composite Parts
Table 2. Composite BioBrick Parts
Part Name Component Description
BBa_K2807003 rAPOBEC1-XTEN-dPspCas13b The completed Editor system performing C-to-U conversion on targeted mRNA sequences.
BBa_K2807012 eGFP-T2A-mCherry The completed Dual-Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807013 eGFP-T2A-mCherry mutant The completed Dual-Reporter system with single base mutation in start codon of eGFP. Used to evaluate base-editing efficiency.
BBa_K2807014 eGFP-GLB1 WT The completed GLB-Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807015 eGFP-GLB1 CCG mutant The completed GLB-Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.
BBa_K2807016 eGFP-GLB1 TCT mutant The completed GLB-Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.