EstebanDLPT (Talk | contribs) |
EstebanDLPT (Talk | contribs) |
||
Line 1: | Line 1: | ||
− | {{Tec-Monterrey}} | + | {{:Team:Tec-Monterrey/Templates/Tec-Monterrey_General_Format}} |
<html> | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | #globalWrapper { | ||
+ | background: rgb(248,247,246); | ||
+ | } | ||
+ | #myImage { | ||
+ | position:absolute; | ||
+ | top: 0; | ||
+ | right: 0; | ||
+ | max-width: 100%; | ||
+ | } | ||
+ | .masthead { | ||
+ | min-height: 30rem; | ||
+ | position: relative; | ||
+ | width: 100%; | ||
+ | padding-top: 8rem; | ||
+ | padding-bottom: 8rem; | ||
+ | background: rgb(248,247,246); | ||
+ | } | ||
+ | .masthead h1 { | ||
+ | font-size: 4rem; | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | } | ||
+ | @media (min-width: 992px) { | ||
+ | .masthead { | ||
+ | height: 100vh; | ||
+ | } | ||
+ | .masthead h1 { | ||
+ | font-size: 5.5rem; | ||
+ | } | ||
+ | } | ||
+ | #project { | ||
+ | -webkit-transition: height 4s; | ||
+ | -moz-transition: height 4s; | ||
+ | -ms-transition: height 4s; | ||
+ | -o-transition: height 4s; | ||
+ | transition: height 4s; | ||
+ | height: auto; | ||
+ | background-image: url('/img/logoOficialCirculos.png'); | ||
+ | background-repeat: no-repeat; | ||
+ | background-position: center; | ||
+ | background-size: contain; | ||
+ | text-align: center; | ||
+ | } | ||
+ | #team { | ||
+ | text-align: center; | ||
+ | } | ||
+ | #myTable{ | ||
+ | width: 95%; | ||
+ | margin: 0px auto !important; | ||
+ | padding: 10px; | ||
+ | border: 2px solid rgb(1,99,60) !important; | ||
+ | border-collapse: collapse; | ||
+ | vertical-align: middle !important; | ||
+ | table-layout: fixed; | ||
+ | } | ||
+ | #myTable td{ | ||
+ | width: 33%; | ||
+ | border: 2px solid rgb(1,99,60) !important; | ||
+ | border-collapse: collapse; | ||
+ | text-align: center; | ||
+ | padding: 5px; | ||
+ | } | ||
− | + | #myTable p { | |
− | + | text-align:center; | |
− | + | font-size: 14px; | |
− | + | } | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | #HP{ | |
− | + | background: rgb(248,247,246); | |
− | + | } | |
− | + | .service-icon { | |
+ | background-color: #fff; | ||
+ | color: #1D809F; | ||
+ | height: 7rem; | ||
+ | width: 7rem; | ||
+ | display: block; | ||
+ | line-height: 7.5rem; | ||
+ | font-size: 2.25rem; | ||
+ | box-shadow: 0 3px 3px 0 rgba(0, 0, 0, 0.1); | ||
+ | } | ||
− | + | .callout { | |
− | + | padding: 15rem 0; | |
− | + | background: linear-gradient(90deg, rgba(255, 255, 255, 0.1) 0%, rgba(255, 255, 255, 0.1) 100%), url("../img/bg-callout.jpg"); | |
− | + | background-position: center center; | |
+ | background-repeat: no-repeat; | ||
+ | background-size: cover; | ||
+ | } | ||
− | + | .callout h2 { | |
− | + | font-size: 3.5rem; | |
− | + | font-weight: 700; | |
− | + | display: block; | |
+ | max-width: 30rem; | ||
+ | } | ||
− | + | .portfolio-item { | |
− | + | display: block; | |
− | + | position: relative; | |
− | + | overflow: hidden; | |
+ | max-width: 530px; | ||
+ | margin: auto auto 1rem; | ||
+ | } | ||
− | + | .portfolio-item .caption { | |
− | + | display: flex; | |
− | + | height: 100%; | |
− | + | width: 100%; | |
+ | background-color: rgba(33, 37, 41, 0.2); | ||
+ | position: absolute; | ||
+ | top: 0; | ||
+ | bottom: 0; | ||
+ | z-index: 1; | ||
+ | } | ||
− | + | .portfolio-item .caption .caption-content { | |
− | + | color: #fff; | |
− | + | margin: auto 2rem 2rem; | |
− | + | } | |
− | < | + | .portfolio-item .caption .caption-content h2 { |
− | < | + | font-size: 0.8rem; |
− | < | + | text-transform: uppercase; |
− | </ | + | } |
+ | </style> | ||
+ | </head> | ||
+ | <body id="page-top"> | ||
+ | <!-- Header --> | ||
+ | <header class="masthead" style="border-bottom: 20px solid #2f6361;"> | ||
+ | <img id="myImage" src="https://static.igem.org/mediawiki/2018/8/82/T--Tec-Monterrey--Tec_ECoding_background.jpeg"> | ||
+ | <div class="container text-center my-auto" style="position: relative; top: 60%; left: 9%; max-width: 50%;"> | ||
+ | <a class="btn btn-primary btn-xl" href="#project">Learn about the project</a> | ||
+ | </div> | ||
+ | <div class="overlay"></div> | ||
+ | </header> | ||
− | < | + | <!-- Project --> |
− | < | + | <section id="project" style="background: #d3edf2; text-align: center;"> |
− | < | + | <div style="width: 80%; margin: auto; text-align: justify; background: #f2fafc;"> |
− | </ | + | <div style="width: 96%; margin: auto; text-align: justify; background: #fdfdfd; padding-top: 10px;"> |
+ | <div style="border-left: 5px solid #2f6361; padding: 20px 0px 20px 20px; margin: 0px 0px 0px 10px;"> | ||
+ | <div class="header-title" style="color: #3582d2;"> | ||
+ | E-Coding | ||
+ | </div> | ||
+ | <div class="header-subtitle" style="color: #5da565;"> | ||
+ | Storing the world one base at a time | ||
+ | </div> | ||
+ | </div> | ||
+ | <div style="border-top: 5px solid #397471; border-right: 5px solid #2a8481; padding: 20px 20px 20px 20px; margin: 0px 10px 0px 10px; color: #2f6361;"> | ||
+ | The research for storing vast amounts of information in smaller devices has been an attention seeking topic in multidisciplinary areas of knowledge all around the world. | ||
+ | <br> | ||
+ | <br> | ||
+ | <q>DNA is an attractive target for information storage because of its capacity for high-density information encoding, longevity under easily achieved conditions, and proven track record as an information bearer</q> <br> Baum, 1995 | ||
+ | </div> | ||
+ | <div style="padding: 0px 20px 20px 20px; margin: 0px 10px 0px 10px; color: #2f6361;"> | ||
+ | Nevertheless, this potential has been limited due to the lack of efficient editing tools. CRISPR-Cas has become popular as an editing tool for its high specificity, low cost, and easy handling, compared to other editing techniques. In our work, we use Cas1-Cas2, proteins in charge of new protospacer adquisition in the CRISPR array. Predesigned sequences (-70pb aprox) in the form of ssDNA are produced in E.coli by induction of a promoter, and adquired by the complex Cas1-Cas2 to be integrated into the array. To produce this oligonucleotides, we use the retrotranscriptase EC86. A subsequent sequencing of the array will confirm the integration of the oligos in the CRISPR locus. We measure the integration rate in terms of stimuli intensity, and time. We hypothise that this system can store ordered data in vivo of many stimuli, just by changing the promoter. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
− | < | + | <!-- Team--> |
− | <h6> | + | <section id="team" style="border-bottom: 20px solid #2f6361; background:#d3edf2;"> |
+ | <div style="width: 80%; margin: 0 auto; text-align: justify; background: #f2fafc;"> | ||
+ | <div style="width: 96%; margin: 0 auto; text-align: justify; background: #fdfdfd; padding-top: 20px; padding-bottom: 20px;"> | ||
+ | <div> | ||
+ | <table align="center" id="myTable"> | ||
+ | <tr> | ||
+ | <th colspan="3" style="text-align:center;"><h5>Team Members</h5></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <h6>Esteban de la Peña <a href="https://www.linkedin.com/in/esteban-de-la-pe%C3%B1a-th%C3%A9venet/" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
+ | <p>In charge of making the wiki</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Alan Ávila <a href="https://www.linkedin.com/in/alanavilarmz/" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
+ | <p>Team Management/Wet Lab</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Adrián Hernández <a href= "https://www.linkedin.com/in/adri%C3%A1n-hern%C3%A1ndez-mendoza-0184ba14b/" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
+ | <p>Interlab managment & Dry Lab</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <h6>Sofia Lara <a href= "https://www.linkedin.com/in/sof%C3%ADa-lara-507773123" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
<p>In charge of Human Practices</p> | <p>In charge of Human Practices</p> | ||
− | </ | + | </td> |
− | + | <td> | |
− | < | + | <h6>José Arnulfo Juárez Figueroa <a href= "https://www.linkedin.com/in/jos%C3%A9-arnulfo-ju%C3%A1rez-figueroa-1478a1159/" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> |
+ | <p>Team Management</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Ana E. Lasso de la Vega</h6> | ||
+ | <p>Team Management</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
<h6>Miranda Iriarte</h6> | <h6>Miranda Iriarte</h6> | ||
<p>Congress of Sciences iGEM</p> | <p>Congress of Sciences iGEM</p> | ||
− | </ | + | </td> |
− | + | <td> | |
− | < | + | |
<h6>Valeria Fuentes</h6> | <h6>Valeria Fuentes</h6> | ||
<p>Congress of Sciences iGEM</p> | <p>Congress of Sciences iGEM</p> | ||
− | </ | + | </td> |
− | + | <td> | |
− | < | + | <h6>Juan Fernández <a href= "https://www.linkedin.com/in/juanfernandezdelagarza/" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> |
+ | <p>Mathematical Model</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
<h6>Norma Garza</h6> | <h6>Norma Garza</h6> | ||
<p>Interlab managment/Drylab</p> | <p>Interlab managment/Drylab</p> | ||
− | </ | + | </td> |
− | + | <td> | |
− | < | + | <h6>Miriam Salas Ramírez</h6> |
+ | <p>Congress of Sciences iGEM</p> | ||
+ | </td> | ||
+ | <td> | ||
<h6>Victor Robledo</h6> | <h6>Victor Robledo</h6> | ||
<p>Wetlab/Drylab</p> | <p>Wetlab/Drylab</p> | ||
− | </ | + | </td> |
− | </ | + | </tr> |
+ | <tr> | ||
+ | <td> | ||
+ | <h6>Carlos Javier Cordero <a href= "https://www.linkedin.com/in/carlos-javier-cordero-oropeza-386593a9/" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
+ | <p>Drylab</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Nora Torres</h6> | ||
+ | <p>Team Management</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Roberto Vásquez</h6> | ||
+ | <p>Mathematical Model</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <h6>Samantha Peña</h6> | ||
+ | <p>Human Practices, and design</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Andrés Sánchez <a href= "http://linkedin.com/in/andresbsanchez" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
+ | <p>Mathematical Model</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Diego Valadez</h6> | ||
+ | <p>Wiki/Dry Lab</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <h6>Jesús Héctor López <a href= "https://www.linkedin.com/in/jesushectorlopezgrajeda" target="_blank"><i class="fa fa-linkedin-square"></i></a></h6> | ||
+ | <p>Dry Lab</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>Adriana Lizeth Rubio</h6> | ||
+ | <p>Dry Lab</p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <h6>María Eugenia Reyna</h6> | ||
+ | <p>Wetlab</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> | ||
− | |||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
+ | </section> | ||
− | + | <!-- Human Practices --> | |
− | < | + | <section id="HP" style="text-align: center;"> |
− | < | + | <div style="width: 80%; display: inline-block; margin: 20px auto 10px auto;"> |
− | + | <div style="width: 40%; float: left; padding-right: 20px;"> | |
− | + | <img src="https://static.igem.org/mediawiki/2018/5/53/T--Tec-Monterrey--Imagen_Memo_Pensante.jpeg"> | |
− | + | </div> | |
− | + | <div style="width: 60%; float: right; padding-top: 60px"> | |
− | <div | + | <div class="header-title" style="color: #3582d2;"> |
− | + | Human Practices | |
− | + | </div> | |
− | + | <div class="header-subtitle" style="color: #5da565;"> | |
− | + | Pollutants in Chiapas and Mexico | |
− | + | </div> | |
− | + | <p style="font-size: 18px; color: #2f6361;">Our project has many applications, but we decided to face a critical environmental problem in one southern state of Mexico: Chiapas. Chiapas is a beautiful state with megadiverse ecosystems, but it also has a severe water pollution problem that affects many communities close to the natural and polluted water sources. With the help of Cecropia and CONANP we will obtain water samples from the polluted sources and analyse them with several techniques in order to make a valuable overview.</p> | |
− | + | </div> | |
− | + | </div> | |
− | + | </section> | |
− | + | </body> | |
− | + | ||
− | + | ||
− | + | ||
− | <div | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </div> | + | |
− | + | ||
− | + | ||
− | + | ||
− | <div | + | |
− | <div class=" | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </div> | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | <div class=" | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </div> | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</html> | </html> | ||
+ | {{:Team:Tec-Monterrey/Templates/Tec-Monterrey_Footer}} |
Revision as of 15:05, 26 June 2018
E-Coding
Storing the world one base at a time
The research for storing vast amounts of information in smaller devices has been an attention seeking topic in multidisciplinary areas of knowledge all around the world.
Baum, 1995
DNA is an attractive target for information storage because of its capacity for high-density information encoding, longevity under easily achieved conditions, and proven track record as an information bearer
Baum, 1995
Nevertheless, this potential has been limited due to the lack of efficient editing tools. CRISPR-Cas has become popular as an editing tool for its high specificity, low cost, and easy handling, compared to other editing techniques. In our work, we use Cas1-Cas2, proteins in charge of new protospacer adquisition in the CRISPR array. Predesigned sequences (-70pb aprox) in the form of ssDNA are produced in E.coli by induction of a promoter, and adquired by the complex Cas1-Cas2 to be integrated into the array. To produce this oligonucleotides, we use the retrotranscriptase EC86. A subsequent sequencing of the array will confirm the integration of the oligos in the CRISPR locus. We measure the integration rate in terms of stimuli intensity, and time. We hypothise that this system can store ordered data in vivo of many stimuli, just by changing the promoter.
Human Practices
Pollutants in Chiapas and Mexico
Our project has many applications, but we decided to face a critical environmental problem in one southern state of Mexico: Chiapas. Chiapas is a beautiful state with megadiverse ecosystems, but it also has a severe water pollution problem that affects many communities close to the natural and polluted water sources. With the help of Cecropia and CONANP we will obtain water samples from the polluted sources and analyse them with several techniques in order to make a valuable overview.