Difference between revisions of "Team:HZAU-China/Attributions"

Revision as of 13:23, 16 October 2018

Attributions

Our project is designed by Zhujun Xia and all circuits is designed by Zhujun Xia, YinQing Zeng and Mo Qiqin.

Wet Lab

Gene Editing

SifA and SipD knock out is completed by Zhujun Xia, Zhuoqi Huang and Wenxin Hu also gave assistance.

Plasmid Construction

Intracellular Environment-Dependent Circuits:

The intracellular environment-dependent circuits is designed by Mo Qiqin and Zhujun Xia.

The intracellular environment-dependent circuits is constructed by Mo Qiqin. Ruonan Tian also gave assistance.

Chemical Control System:

The ATc induced chemical control system is constructed by He Yang, Zhuoqi Huang also gave assistance.

The TetR-eGFP fusion protein expression system for TetR expression measuring is constructed by He Yang, Zhuoqi Huang also gave assistance.

Targeting Specificity and Surface-Displaying:

RGD-inserted OmpA circuit clone and construction of Salmonella SL1344_RS05255 (OmpA coding sequence) are completed by YinQing Zeng and Lingyu Zhong.

The circuit of surface-display system is constructed by Lingyu Zhong. Optimation of OmpA in E. Coli is completed by Zhujun Xia.

Plasmid Standardization:

Plasmid standardization is completed by Zhiqing Guo, Zhuoqi Huang by using 3A Assembly.

Plasmid Submission:

Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and three GSDMD full length mutations) are constructed by Zhiqing Guo and Wenxin Hu.

The description of submitted plasmids by ATP assay and cell phenotype via transfection is de-signed and completed by Zhujun Xia.

Bacterial Phenotype Verification

Bacterial phenotype verification are designed and completed by He Yang, including the OD measurement and CFU measurement of chemical control system.

Cell Phenotype Verification

Cell phenotype verifications are designed and completed by Zhujun Xia, including the verifi-cation of chemical control system, and targeting specificity, all these circuits and systems work so well.

Bio-Safety Verification

Safety verification is designed and completed by Zhujun Xia, which demonstrated our project is safe enough to be used.

InterLab

InterLab is conducted by Xichen Rao, Zhujun Xia, Ruonan Tian and Heng Heng also gave assistance, our data passed the check of the Measurement Committee successfully.

Dry Lab

Mathematical model and app of salmonella infection and the releasing of GSDMD are built by Songtao Chen. ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD is built by Xichen Rao. The modeling of adjusting the strength of RBS and ATc with fixed GSDMD-N concentration is completed by Yini Miao. The future work is confirming the threshold of GSDMD-N275. The modeling of the promoter sifA and promoter entC is completed by Ruonan Tian.

Software

The applet of “Bacterial Colony Counter” is designed and programmed by Shuguang Wang.

Human Practice

Collaboration and communication with other teams is completed by YinQing Zeng. Handicraft manufacture is designed and completed by YinQing Zeng. Questionnaire preparation, the advertisement of synthetic biology and popular science articles written is completed by Heng Heng. Two posters for Eurasian exchange meetings and CCIC is completed by Ruonan Tian.

Website

Our intergrative wiki is designed and programmed by Shuguang Wang and the artist is designed by Yuying Xiang.

Our blog is built by Shuguang Wang.

Others

The plate reader machine operation is conducted by Mo Qiqin. Chief cleaner of the laboratory is Xichen Rao.

Acknowledgement

For each parts of experiments, our appreciations are as follow:

Cell lines

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the cell lines of HEK293T, HELA, MCF7, iBMDM, our experiment can process successfully.

Thanks to Dr. Feng Shao’s laboratory, for sharing the cell line of GSDMD-/- HELA cells, it provides us great support.

Thanks to Dr. Yi Zeng and Dr. Ms Hong Fan, Shanghai Institute of Biochemistry and Cell Biology, CAS, for sharing the cell line of MDA-MB-231.

Bacteria

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the strain of Salmonella enterica var. Typhimurium SL1344.

Thanks to Dr. Chenli Liu from SIAT CSynBER’s generous share (Strain: E.coli K-12 MG1655 with mCherry in the genome), our experiment can process successfully.

Gene Knock In and Knock Out

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for providing the protocol and material of gene editing based on two-step allelic exchange, it provides us great help.

Thanks to Pan Chu, team leader of iGEM-2016, Huazhong Agricultural University, for teaching us multigene editing based on CRISPR and λ-RED.

Plasmid Backbone

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the plasmid backbone (Pcs2-eGFP) to us.

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