Difference between revisions of "Team:Jiangnan/Hardware"

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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Safety">Safety</a>
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</div>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Hardware">Hardware</a>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Team">Team</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Team">Team Members</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Attributions">Attribution</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Collaborations">Collaboration</a></li>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Human Practice</a>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Overview</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Silver">Silver</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Gold</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Public_Engagement">Pulic Engagement</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Entrepreneurship</a></li>
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<a href="https://2018.igem.org/Team:Jiangnan/Model">Model</a>
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<a href="https://2018.igem.org/Team:Jiangnan/Notebook">Notebook</a>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Notebook">Lab Book</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Protocol">Protocol</a></li>
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</ul>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan">Project</a>
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Background">Background</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Design">Design</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Demonstrate">Demonstration</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Results">Result</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Parts">Part</a></li>
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</ul>
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</div>
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</div>
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<div class="navlogo" style="float: left;width: 20%;text-align: center;">
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<a href="https://2018.igem.org/Team:Jiangnan"><img src="https://static.igem.org/mediawiki/2018/d/d7/T--Jiangnan--igemJN_logo.png" style="width: 3em;"></a>
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<img src="https://static.igem.org/mediawiki/2018/7/73/T--Jiangnan--device_top.png" width="100%">
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<h3 class="Jbule">Device-plasma</h3>
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<h4 class="Jbule">Configuration</h4>
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<h1>Hardware</h1>
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<p>The plasma equipment consists of a helium bottle, a rotor flowmeter (which monitors gas flow), high and low voltage outlets, a power supply and an oscilloscope (which displays voltage and current conditions). <br>
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When using this device, we place samples under the ejection nozzle, open the helium bottle, turn the power until the rotor flowmeter reaches the desired parameters (0.6-0.8 m/h, 20℃, 101325 MPa) and the oscilloscope displays 110V.</p>
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<p></p>
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<h4 class="Jbule">Protocol</h4>
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<p>1 ml blank DMEM medium was added to the 12-hole plate and placed directly under the low temperature atmospheric pressure plasma generator. The distance between the end of plasma beam and the medium is 1 cm, the injection port is vertically aligned to the center of the hole in the 12-hole plate, the controlled helium flow rate is 1 slm / min, and the output voltage of the high frequency power supply controlled by the transformer is 0.96-1.24 Kv. Plasma activated medium (PAM) was obtained by repeating the aforementioned process. When cells were incubated with viruses, the active oxygen and the active nitrogen in the culture medium had a beneficial effect on virus replication. 500000 MDBK cells were placed in the 6-hole plate for 24 hours. Then the original culture medium was changed to 1 ml PAM (DMEM activated by plasma). IBRV were then added and incubated with MDBK cells for 1 hour before changing the medium to the maintaining 1640 medium (supplemented with serum at 2% final concentration). The culture was collected for virus titer detection after cell lysis.</p>
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<p></p>
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<h4 class="Jbule">Result</h4>
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<p>After incubating IRF7-silenced cells with the DMEM medium for 1 hour, IBRV replication in MDBK was further increased 2.5 folds. </p>
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<p>Advantages of using plasma activated medium in cell treatment:<br>
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(1) The intensity is controllable and the results are stable.<br>
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(2) The approach is simple and fast, which takes about 2 min for the treatment.<br>
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(3) The efficacy is obvious, which easily increase virus titer 2.5 folds further. </p>
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</div>
 
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<h3>Best Hardware Special Prize</h3>
 
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<p>iGEM is about making teams of students making synthetic biology projects. We encourage teams to work with parts and build biological devices in the lab. But we are inclusive and want all teams to work on many other types of problems in synbio. Robotic assembly, microfluidics, low cost equipment and measurement hardware are all areas ripe for innovation in synbio. </p>
 
  
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<p>
 
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Teams who are interested in working with hardware as a side project are encouraged to apply for the hardware award.
 
  
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<br><br>
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<div style="text-align: center;">
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To compete for the <a href="https://2018.igem.org/Judging/Awards">Best Hardware prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>.
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<div style="width: 100%">
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<br><br>
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<img src="https://static.igem.org/mediawiki/2018/6/67/T--Jiangnan--device_plasmadevice.png" width="40%">
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You must also delete the message box on the top of this page to be eligible for this prize.
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<img src="https://static.igem.org/mediawiki/2018/0/0c/T--Jiangnan--device_plasma.jpeg" width="40%">
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</p>
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<p style="color: #64b5f6"><b>Figure 1.</b> Configuration of cold atmospheric plasma source.</p>
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</p>
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</div>
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<img src="https://static.igem.org/mediawiki/2018/2/2b/T--Jiangnan--device_pic2.jpeg" width="70%">
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<p style="color: #ef6c00 "><b>Figure 2.</b> Protocol of cold atmospheric plasma activated medium preparation.</p>
 
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<div class="highlight decoration_A_full">
 
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<h3>Inspiration</h3>
 
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<p>You can look at what other teams did to get some inspiration! <br />
 
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Here are a few examples:</p>
 
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<ul>
 
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<li><a href="https://2016.igem.org/Team:Valencia_UPV">2016 Valencia UPV</a></li>
 
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<li><a href="https://2016.igem.org/Team:Aachen">2016 Aachen </a></li>
 
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<li><a href="https://2015.igem.org/Team:TU_Delft">2015 TU Delft  </a></li>
 
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<li><a href="https://2015.igem.org/Team:TU_Darmstadt">2015 TU Darmstadt</a></li>
 
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Revision as of 14:16, 16 October 2018

Safety
Hardware
Team
Human Practice
Model
Notebook
Project

Device-plasma

Configuration

The plasma equipment consists of a helium bottle, a rotor flowmeter (which monitors gas flow), high and low voltage outlets, a power supply and an oscilloscope (which displays voltage and current conditions).
When using this device, we place samples under the ejection nozzle, open the helium bottle, turn the power until the rotor flowmeter reaches the desired parameters (0.6-0.8 m/h, 20℃, 101325 MPa) and the oscilloscope displays 110V.

Protocol

1 ml blank DMEM medium was added to the 12-hole plate and placed directly under the low temperature atmospheric pressure plasma generator. The distance between the end of plasma beam and the medium is 1 cm, the injection port is vertically aligned to the center of the hole in the 12-hole plate, the controlled helium flow rate is 1 slm / min, and the output voltage of the high frequency power supply controlled by the transformer is 0.96-1.24 Kv. Plasma activated medium (PAM) was obtained by repeating the aforementioned process. When cells were incubated with viruses, the active oxygen and the active nitrogen in the culture medium had a beneficial effect on virus replication. 500000 MDBK cells were placed in the 6-hole plate for 24 hours. Then the original culture medium was changed to 1 ml PAM (DMEM activated by plasma). IBRV were then added and incubated with MDBK cells for 1 hour before changing the medium to the maintaining 1640 medium (supplemented with serum at 2% final concentration). The culture was collected for virus titer detection after cell lysis.

Result

After incubating IRF7-silenced cells with the DMEM medium for 1 hour, IBRV replication in MDBK was further increased 2.5 folds.

Advantages of using plasma activated medium in cell treatment:
(1) The intensity is controllable and the results are stable.
(2) The approach is simple and fast, which takes about 2 min for the treatment.
(3) The efficacy is obvious, which easily increase virus titer 2.5 folds further.

Figure 1. Configuration of cold atmospheric plasma source.

Figure 2. Protocol of cold atmospheric plasma activated medium preparation.