Difference between revisions of "Team:SSTi-SZGD"

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<h1> Welcome to iGEM 2018! </h1>
 
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<h3 class="title">Abstract</h3>
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The production of hyaluronic acid has been changed from traditional animal tissue formulation to microbial fermentation. However, there is no report that tissue cells or microorganisms can di-rectly produce low molecular weight hyaluronic acid. In order to prepare low molecular weight hyaluronic acid, physical and chemical methods are needed. However, there are many drawbacks in physical and chemical methods, such as poor product stability, low efficiency, complex reac-tion conditions and possible environmental pollution.
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This year our project constructed a recombinant strain Bacillus subtilis 168E which could directly produce different molecular weight hyaluronic acid products by regulating the activities of LHAase. Bacillus subtilis 168 was selected as the best chassis. The HasA gene and identified pre-cursor  genes （tuaD , gtaB , gluM , glmM and glmS）was transferred into Bacillus subtilis. Since hyaluronic acid of high molecular weight was produced at this time, we transferred the LHAase gene into Bacillus subtilis 168 which is from leech resources coding hyaluronidase. Therefore the hyaluronic acid could be enzymatic hydrolyzed to different molecular weight.
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Our intended application is use low molecular weight hyaluronic acid to prepare microneedles and that hyaluronic acid can be dissolved and stuck in the skin under the action of body fluid when it is punctured into the human skin, microneedles allow the penetration of low molecular weight HA through to the dermal papillary cutis, which could not only play a cosmetic role. Be-cause hyaluronic acid is an endogenous substance of the human body and does not cause the in-flammatory reaction, therefore it is a new type of microneedle material and has a potential mar-ket.
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<h3>Before you start</h3>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Revision as of 03:01, 27 June 2018

Hyaluronic acid micro factory : A bacterium produces low molecular weight hyaluronic acid
Engineering strain : Bacillus subtilis
Genetic origin : Streptococcus equi and Leech
READ MORE

Abstract

The production of hyaluronic acid has been changed from traditional animal tissue formulation to microbial fermentation. However, there is no report that tissue cells or microorganisms can di-rectly produce low molecular weight hyaluronic acid. In order to prepare low molecular weight hyaluronic acid, physical and chemical methods are needed. However, there are many drawbacks in physical and chemical methods, such as poor product stability, low efficiency, complex reac-tion conditions and possible environmental pollution.

This year our project constructed a recombinant strain Bacillus subtilis 168E which could directly produce different molecular weight hyaluronic acid products by regulating the activities of LHAase. Bacillus subtilis 168 was selected as the best chassis. The HasA gene and identified pre-cursor genes （tuaD , gtaB , gluM , glmM and glmS）was transferred into Bacillus subtilis. Since hyaluronic acid of high molecular weight was produced at this time, we transferred the LHAase gene into Bacillus subtilis 168 which is from leech resources coding hyaluronidase. Therefore the hyaluronic acid could be enzymatic hydrolyzed to different molecular weight.

Our intended application is use low molecular weight hyaluronic acid to prepare microneedles and that hyaluronic acid can be dissolved and stuck in the skin under the action of body fluid when it is punctured into the human skin, microneedles allow the penetration of low molecular weight HA through to the dermal papillary cutis, which could not only play a cosmetic role. Be-cause hyaluronic acid is an endogenous substance of the human body and does not cause the in-flammatory reaction, therefore it is a new type of microneedle material and has a potential mar-ket.