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| − | <html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:dt="uuid:C2F41010-65B3-11d1-A29F-00AA00C14882" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=gb2312" ><meta name=ProgId content=Word.Document ><meta name=Generator content="Microsoft Word 14" ><meta name=Originator content="Microsoft Word 14" ><title></title><!--[if gte mso 9]><xml><o:DocumentProperties><o:Author>³Â ÓñÈã</o:Author><o:LastAuthor>²Ü½ø»Ô</o:LastAuthor><o:Revision>1</o:Revision><o:Pages>4</o:Pages><o:Characters>7732</o:Characters><o:Lines>1</o:Lines><o:Paragraphs>53</o:Paragraphs></o:DocumentProperties><o:CustomDocumentProperties><o:KSOProductBuildVer dt:dt="string" >2052-10.1.0.7400</o:KSOProductBuildVer></o:CustomDocumentProperties></xml><![endif]--><!--[if gte mso 9]><xml><o:OfficeDocumentSettings></o:OfficeDocumentSettings></xml><![endif]--><!--[if gte mso 9]><xml><w:WordDocument><w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel><w:DisplayHorizontalDrawingGridEvery>0</w:DisplayHorizontalDrawingGridEvery><w:DisplayVerticalDrawingGridEvery>2</w:DisplayVerticalDrawingGridEvery><w:DocumentKind>DocumentNotSpecified</w:DocumentKind><w:DrawingGridVerticalSpacing>7.8 °õ</w:DrawingGridVerticalSpacing><w:PunctuationKerning></w:PunctuationKerning><w:View>Web</w:View><w:Compatibility><w:DontGrowAutofit/><w:BalanceSingleByteDoubleByteWidth/><w:DoNotExpandShiftReturn/><w:UseFELayout/></w:Compatibility><w:Zoom>0</w:Zoom></w:WordDocument></xml><![endif]--><!--[if gte mso 9]><xml><w:LatentStyles DefLockedState="false" DefUnhideWhenUsed="true" DefSemiHidden="true" DefQFormat="false" DefPriority="99" LatentStyleCount="260" > | + | <iframe src="https://static.igem.org/mediawiki/2018/b/b9/T--AHUT_China--_56421548.pdf" width="800" height="600"></iframe> |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Normal" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="9" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="heading 1" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 2" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 3" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 4" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 5" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 6" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 7" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 8" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="heading 9" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 1" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 2" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 3" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 4" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 5" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 6" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 7" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 8" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index 9" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 1" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 7" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 8" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toc 9" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Normal Indent" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="footnote text" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="annotation text" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="header" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="footer" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="index heading" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" QFormat="true" Name="caption" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="table of figures" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="envelope address" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="envelope return" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="footnote reference" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="annotation reference" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="line number" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="page number" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="endnote reference" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="endnote text" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="table of authorities" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="macro" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="toa heading" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Bullet" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Number" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Bullet 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Bullet 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Bullet 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Bullet 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Number 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Number 3" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Number 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Number 5" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Title" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Closing" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Signature" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="1" SemiHidden="false" UnhideWhenUsed="false" Name="Default Paragraph Font" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text Indent" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Continue" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Continue 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Continue 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Continue 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="List Continue 5" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Message Header" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Subtitle" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Salutation" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Date" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text First Indent" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text First Indent 2" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Note Heading" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text Indent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Body Text Indent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Block Text" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Hyperlink" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="FollowedHyperlink" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Strong" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Emphasis" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Document Map" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Plain Text" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="E-mail Signature" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Normal (Web)" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Acronym" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Address" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Cite" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Code" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Definition" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Keyboard" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Preformatted" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Sample" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Typewriter" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="HTML Variable" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" UnhideWhenUsed="false" Name="Normal Table" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="annotation subject" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="No List" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="1 / a / i" ></w:LsdException>
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| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="1 / 1.1 / 1.1.1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Article / Section" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Simple 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Simple 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Simple 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Classic 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Classic 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Classic 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Classic 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Colorful 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Colorful 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Colorful 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Columns 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Columns 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Columns 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Columns 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Columns 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 7" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid 8" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 7" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table List 8" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table 3D effects 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table 3D effects 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table 3D effects 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Contemporary" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Elegant" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Professional" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Subtle 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Subtle 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Web 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Web 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Web 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Balloon Text" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Grid" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" Name="Table Theme" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Placeholder Text" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="1" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="No Spacing" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="List Paragraph" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Quote" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Intense Quote" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3 Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid Accent 1" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3 Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid Accent 2" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3 Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid Accent 3" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3 Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid Accent 4" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3 Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid Accent 5" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false" UnhideWhenUsed="false" Name="Light Shading Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false" UnhideWhenUsed="false" Name="Light List Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false" UnhideWhenUsed="false" Name="Light Grid Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 1 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Shading 2 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 1 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false" UnhideWhenUsed="false" Name="Medium List 2 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 1 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 2 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false" UnhideWhenUsed="false" Name="Medium Grid 3 Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Shading Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful List Accent 6" ></w:LsdException>
| + | |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false" UnhideWhenUsed="false" Name="Colorful Grid Accent 6" ></w:LsdException>
| + | |
| − | </w:LatentStyles></xml><![endif]--><style>
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| − | text-underline:single;
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| − | color:blue;
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| − | color:red;
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| − | table.MsoNormalTable{
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| − | div.Section0{page:Section0;}</style></head><body style="tab-interval:21pt;text-justify-trim:punctuation;" ><!--StartFragment--><div class="Section0" style="layout-grid:15.6000pt;" ><h1 align=center style="text-align:center;" ><b><span style="mso-spacerun:'yes';font-family:µÈÏß;mso-bidi-font-family:ËÎÌå;font-weight:bold;font-size:22.0000pt;mso-font-kerning:22.0000pt;" ><font face="µÈÏß" >±³¾°½éÉÜ</font></span></b><b><span style="mso-spacerun:'yes';font-family:µÈÏß;mso-bidi-font-family:ËÎÌå;font-weight:bold;font-size:22.0000pt;mso-font-kerning:22.0000pt;" ><o:p></o:p></span></b></h1><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >Èç½ñÎÂÊÒЧӦÊÇÈËÃÇÃæÁÙµÄÍ·ºÅÎÊÌ⣬Êܵ½¸÷¹úÕþ¸®µÄ¼«´ó¹Ø×¢¡£</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >´óÔ¼Õ¼ÎÂÊÒÆøÌå×ÜÁ¿µÄ</font>2/3<font face="ËÎÌå" >£¬ÊÇÒýÆðÎÂÊÒЧӦµÄ×îÖ÷ÒªµÄÆøÌå</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >1</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¡£Òò´Ë¸÷ÖÖ¿ØÖƶþÑõ»¯Ì¼ÅŷŵĴëÊ©£¨Èç</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >²¶¼¯£©¿ªÊ¼Ô½À´Ô½Êܵ½ÖØÊÓ¡£</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >²¶¼¯µÄÖ÷Òª·½·¨°üÀ¨£ºÈܼÁÎüÊÕ·¨¡¢ÎïÀíÎü¸½·¨¡¢Ä¤·ÖÀë·¨µÈ¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ÈܼÁÎüÊÕ·¨Ö÷ÒªÊÇ»ùÓÚ»¯Ñ§ÎüÊÕ·¨£¬Ò»Ð©»¯Ñ§ÎüÊÕ¼Á¿ÉÒÔÓë</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >·´Ó¦Ðγɻ¯ºÏÎ²¢´ÓÁ÷¹ýº¬ÓÐÎüÊÕ¼ÁµÄÑ̵ÀÆøÖзÖÀë³ö</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¡£µ«ÊÇ´Ë·½·¨Ò²ÓÐÒÔϼ¸ÖÖȱµã£ºÈÜÒºÒ×Ñõ»¯·Ö½â£»ÈÜÒºµÄ¸¯Ê´ÐÔ½ÏÇ¿£¬Ò׸¯Ê´ÒÇÆ÷£¬ÒÇÆ÷ά³Ö·´Ó¦³É±¾½Ï¸ß£»ÄܺĴó²¢ÇÒÔËÐзÑÓøߡ£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ÎïÀíÎü¸½·¨ÊǸù¾ÝÆøÌåÖв»Í¬×é·Ö¶Ô¹ÌÌåÎü¸½¼ÁµÄÎü¸½ÌØÐÔ²»Í¬½«</font> CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >²¶¼¯ÆðÀ´¡£µ«´Ë·½·¨ÐèÒª´óÁ¿µÄÎü¸½¼ÁÀ´Î¬³Ö´Ë¹ý³ÌµÄÔËÐУ¬ÇÒÎü¸½¼ÁµÄÑ¡ÔñÐԽϲÎü¸½ÈÝÁ¿µÍ£¬Ð§ÂʵÍÏ£¬Ôì³ÉÁËÔËÓª³É±¾ºÜ¸ß£¬Êµ¼ÊÓ¦ÓýÏÉÙ¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >Ĥ·ÖÀë·¨ÊÇ»ùÓھۺϱ¡Ä¤¶Ô²»Í¬µÄÆøÌå×é·ÖµÄÉø͸Âʲ»Í¬À´´ïµ½·ÖÀ벻ͬÆøÌå×é·ÖµÄÄ¿µÄ¡£ËùÓõÄĤ£¬´óÌå¿ÉÒÔ·ÖΪÓлúĤºÍÎÞ»úĤ¡£ÆäÖÐÓлúĤ¶ÔÆøÌå×é·ÖµÄÑ¡ÔñÐÔ½ÏÇ¿£¬×°Åä¼òµ¥£¬µ«ÆäÄÍÈÈÐÔÄܺͷÀ¸¯Ê´ÐÔÄܲ¶øÎÞ»úĤÕýºÃÏà·´£¬ÆäÄÍÈÈÐÔÄܺͷÀ¸¯Ê´ÐÔÄܺ㬵«Æä×°Åä½Ï¸´ÔÓ¡£×ܵÄÀ´¿´£¬ÕâÖÖ·½·¨²¶¼¯µÃµ½µÄ</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µÄ´¿¶È²»¸ß£¬ÐèÒª½øÐжà´Î´¿»¯£¬ÔÚ¹¤ÒµÉÏÓ¦ÓýÏÉÙ¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ÒÔÉϵļ¸ÖÖ</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >²¶¼¯¼¼Êõ¶¼¾ßÓгɱ¾½Ï¸ß¡¢Ð§ÂÊÆ«µÍ¡¢¿ÉÑ»·ÐÔ²îµÄȱµã£¬ÕâЩÎÞ·¨±ÜÃâµÄȱµã×è°ÁËÆäÔÚÉú²úÉú»îÖеÄÓ¦Óá£Òò´Ë¼±ÐèÒªÐÂÐ͵ļ¼Êõµ®Éú£¬¶ø̼Ëáôûø£¨</font>CA<font face="ËÎÌå" >£©²¶¼¯¼¼ÊõµÄ³öÏÖÃÖ²¹ÁËÆäËû·½·¨µÄ²»×ã¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >̼Ëáôûø£¬ÊÇÒ»ÖÖº¬ÓÐ</font>Zn</span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >2+</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µÄ½ðÊôø£¬Ì¼Ëáôûø¿ÉÒÔ´ß»¯</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ºÍ</font>H</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >O<font face="ËÎÌå" >Éú³É</font><font face="Times New Roman" >HCO</font></span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >3</span></sub><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >-</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£¨Èçͼ</font>1<font face="ËÎÌå" >£©¡£ÓëÆäËûÀàÐ͵ÄøÏà±È½Ï£¬Ì¼Ëáôû´ß»¯ËÙÂʷdz£¿ì¡£¸÷ÖÖ̼ËáôûøµÄ´ß»¯ËÙÂʵķ¶Î§ÊÇÿÃë</font><font face="Times New Roman" >10</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >4</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µ½</font>10</span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >6</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¸ö·´Ó¦¡£ÔÚ¸÷ÖÖÀ´Ô´µÄ̼ËáôûøÖУ¬ÈË̼ËáôûøµÄ´ß»¯Ð§ÂÊ×î¸ß¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=16 align=center style="text-align:center;" ><img width="693" height="241" src="IGEM±³¾°½éÉÜ.files/IGEM±³¾°½éÉÜ738.png" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:µÈÏß;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal align=center style="text-indent:24.0000pt;mso-char-indent-count:2.0000;text-align:center;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ͼ</font>1 <font face="ËÎÌå" >̼Ëáôûø</font><font face="Times New Roman" >(CA2)</font><font face="ËÎÌå" >µÄ´ß»¯»úÀí</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >̼ËáôûøµÄ·Ö×ÓÖÊÁ¿Ô¼Îª</font>30KDa<font face="ËÎÌå" >£¬Óɵ¥Ò»ëÄÁ´×é³É£¬°üº¬ÓÐÔ¼</font><font face="Times New Roman" >260</font><font face="ËÎÌå" >¸ö°±»ùËᣬÿ¸öø·Ö×Óº¬ÓÐÒ»¸ö</font><font face="Times New Roman" >Zn</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >2+</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¡£Æä½á¹¹ÎªÍÖÇòÐÍ£¬·Ö×ÓÖв¿ÓÐÒ»¸ö´ü¿ÕÇ»ÉîÔ¼</font> 1.5nm<font face="ËÎÌå" >£¬Ç»¿Ú¿íÔ¼ </font><font face="Times New Roman" >2.0nm</font><font face="ËÎÌå" >£¬</font><font face="Times New Roman" >Zn</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >2+</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >½áºÏÔÚ¿ÕÇ»µ×²¿¡£Ä¿Ç°£¬Ñо¿×î¶àµÄÒ»Àà̼ËáôûøΪ¶¯ÎïÌåÄÚ</font>α-<font face="ËÎÌå" >¼Ò×å </font><font face="Times New Roman" >CA</font><font face="ËÎÌå" >£¬Ò²±»³Æ×÷ </font><font face="Times New Roman" >CA2</font><font face="ËÎÌå" >¡£ËüµÄÖ÷Òª¶þ¼¶½á¹¹Î»ÓÚÆäø·Ö×Ó</font><font face="Times New Roman" >10</font><font face="ËÎÌå" >¸ö</font><font face="Times New Roman" >β-</font><font face="ËÎÌå" >ÕÛµþÖС£ÕýÊÇÓÉÓÚËüÃǵĴæÔÚ£¬Ã¸½á¹¹·ÖΪÁ½²¿·Ö¡£Ã¸·Ö×ÓÖÐÐí¶à¹Ø¼ü°±»ùËá²Ð»ùλµãÓëÆä»îÐÔÓйء£³ýÁË</font><font face="Times New Roman" >β-</font><font face="ËÎÌå" >ÕÛµþÖ®Íâ £¬Ã¸·Ö×ӵıíÃ滹ÒÔ</font><font face="Times New Roman" >α-</font><font face="ËÎÌå" >ÂÝÐý½á¹¹·Ö²¼£¬Æäͨ³£ÊǶÌС½á¹¹£¨Èçͼ</font><font face="Times New Roman" >2</font><font face="ËÎÌå" >£©¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=16 align=center style="text-align:center;" ><img width="567" height="426" src="IGEM±³¾°½éÉÜ.files/IGEM±³¾°½éÉÜ1002.png" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:µÈÏß;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal align=center style="text-indent:24.0000pt;mso-char-indent-count:2.0000;text-align:center;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ͼ</font>2 <font face="ËÎÌå" >̼Ëáôûø£¨</font><font face="Times New Roman" >CA2</font><font face="ËÎÌå" >£©½á¹¹</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >2</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >Ïà½ÏÓÚÆäËû·½·¨£¬Ì¼Ëáôûø²¶¼¯¼¼Êõ£¬¾ßÓÐרһÐÔ£¬¿ÉÒÔ´ÓÆäËûÆøÌåÖÐÌØÒìµÄ²¶¼¯</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¡£ÇÒ´Ë·½·¨±È½Ï»·±££¬Ì¼Ëáôûø°Ñ</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ת±ä³É̼ËáÇâ¸ù£¬Ì¼ËáÇâ¸ù¿ÉÒÔÂú×ãÖ²ÎïºÍ΢ÉúÎïµÄÉú³¤ÐèÇó</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >3</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¡£µ±</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ת±äΪ̼ËáÇâ¸ùʱ£¬Ì¼ËáÇâ¸ù¿ÉÒÔÓë¸ÆÀë×Ó½áºÏÉú³É̼Ëá¸Æ£¬Îȶ¨µØ´¢´æÔÚµØÏ¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >Ïà½ÏÓÚÆäËû·½·¨£¬Ì¼Ëáôûø²¶¼¯¼¼Êõ£¬¸ü¸ßЧ¡£Ò»°ãµÄ</font> CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >²¶¼¯¼¼ÊõÖ÷ÒªµÄÏÞËÙ²½ÖèÊÇ</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µÄË®ºÏ·´Ó¦£¬¶ø̼Ëáôûø¿ÉÒÔ´ó·ù¶ÈÌá¸ß</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µÄË®ºÏ·´Ó¦ËÙÂÊ£¬´Ó¶øÌá¸ß</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µÄ²¶¼¯Ð§ÂÊ</font></span><sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:super;" >4</span></sup><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >̼Ëáôûø²¶¼¯¼¼ÊõËäÈ»¾ßÓиßЧÐÔ£¬µ«ÈÔ¾ßÓÐÒ»¶¨µÄÏÞÖÆ¡£ÒòΪ´ó¶àÊýÌìȻ̼Ëáôûø¶Ô·´Ó¦»·¾³¹ýÓÚÃô¸Ð£¬ÇÒ²»¾ßÓÐÈÈÎȶ¨ÐÔ£¬È»¶ø̼Ëáôûø²ÎÓë</font>CO</span><sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;vertical-align:sub;" >2</span></sub><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >²¶¼¯Ê±</font> ,<font face="ËÎÌå" >»·¾³µÄζÈÒ»°ãΪ</font><font face="Times New Roman" >65</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >℃</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >,<font face="ËÎÌå" >¶øÌìȻ̼ËáôûøÔÚ´ËζÈÏÂÎÞ·¨±£³ÖÎȶ¨£¬ÔÚ¶à´ÎÑ»·Ö®ºóø»îÐÔɥʧ¡£Ì¼ËáôûøµÄ¼Û¸ñ±È½Ï°º¹ó£¬Èô¾³£¸ü»»»áʹ²¶¼¯³É±¾´ó´óÌá¸ß£¬ÕâÏÞÖÆÁË̼Ëáôûø²¶¼¯¼¼ÊõµÄ´ó·¶Î§Íƹ㡣Òò´ËÏÖÔڵĹؼüÊÇÑ°ÕÒÈÈÎȶ¨ÐÔµÄ̼Ëáôûø¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ΪÁËÑ°ÕÒµ½¸ßЧ´ß»¯ºÍ¸ßÎȶ¨ÐÔµÄ̼Ëáôûø£¬ÔÚ±¾ÏîÄ¿ÖУ¬ÎÒÃÇÀûÓÃÈË̼Ëáôûø</font>2<font face="ËÎÌå" >£¨ÒÔϼò³Æ </font><font face="Times New Roman" >CA2</font><font face="ËÎÌå" >£©µÄ¸ßЧ´ß»¯Ìص㣬ÔËÓ÷Ö×ÓÄ£ÄâÊֶζÔÆä°±»ùËáÐòÁнøÐÐÓÅ»¯£¬Éè¼Æ³ö¸ß»îÐÔÇÒ¸ßÎȶ¨ÐÔµÄ</font><font face="Times New Roman" >CA2</font><font face="ËÎÌå" >Í»±äÌå¡£</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ÏîÄ¿°üÀ¨</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ÒÔϼ¸·½ÃæµÄÄÚÈÝ£º</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >1</span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£©·Ö×ÓÄ£Ä⣻</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >2</span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£©±í´ï</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >Ò°ÉúÐͺÍÍ»±äÐÍ</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >CA2<font face="ËÎÌå" >µÄ</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >´ó³¦</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >¸Ë¾ú¾úÖê</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >µÄ¹¹½¨</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£»</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >3</span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£©</font>CA2<font face="ËÎÌå" >µÄ±í´ï</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >Óë´¿»¯</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£»</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="text-indent:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >4</span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£©</font>CA2<font face="ËÎÌå" >µÄ</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >ʵ¼ÊÓ¦ÓÃ</font></span><span style="mso-spacerun:'yes';font-family:ËÎÌå;mso-ascii-font-family:'Times New Roman';mso-hansi-font-family:'Times New Roman';mso-bidi-font-family:'Times New Roman';line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><font face="ËÎÌå" >£º</font>CO2<font face="ËÎÌå" >²¶¼¯¡£</font></span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >1. Rahman F A, Aziz M M A, Saidur R, et al. Pollution to solution: Capture and sequestration of carbon dioxide (CO2) and its utilization as a renewable energy source for a sustainable future[J]. Renewable & Sustainable Energy Reviews, 2017, 71:112-126.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >2. Claudiu T. Supuran. Structure and function of carbonic anhydrases[J]. Biomolecular Biochemical journal, 2016, 473(14):2023-2032.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >3. Lionetto M G, Caricato R, Erroi E, et al. Potential application of carbonic anhydrase activity in bioassay and biomarker studies [J]. Chemistry & Ecology, 2006, 22(sup1): S119-S25.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >4. Migliardini F, De L V, Carginale V, et al. Biomimetic CO2 capture using a highly thermostable bacterial α-carbonic anhydrase immobilized on a polyurethane foam [J]. Journal of Enzyme Inhibition & Medicinal Chemistry, 2014, 29(1): 146.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal align=center style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;text-align:center;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Background</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Nowadays, greenhouse effect is the most important problem that people are facing, which attracts great attention from governments. CO2 accounts for about two-thirds of the total greenhouse gas, and it is the most important gas which causes the greenhouse effect. As a result, various measures to control carbon dioxide emissions, such as CO2 capture, are becoming more and more important. The main methods of CO2 capture include solvent absorption, physical adsorption and membrane separation.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" > Solvent absorption is mainly based on chemical absorption. Some chemical absorbents can react with CO2 to form compounds and separate CO2 from the flue gas containing the absorbent. However, this method also has the following disadvantages: the solution is easy to oxidize and decompose; the solution is highly corrosive and easy to corrode the instrument; large energy consumption and high operation cost.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Physical adsorption method collects CO2 according to the adsorption characteristics of different components of gas to solid adsorbent. However, this method requires a large number of adsorbent to maintain the operation of this process, and the adsorbent has poor selectivity, low adsorption capacity and low efficiency, resulting in high operating costs and few practical applications.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Membrane separation method is based on the polymer film to different gas components of different permeability to achieve the purpose of separation of different gas components. The membranes used can be divided into organic membranes and inorganic membranes. Among them, the organic membrane has strong selectivity for the gas components, simple assembly, but poor heat resistance and corrosion resistance. The inorganic membrane, on the contrary, has good heat resistance and corrosion resistance, but its assembly is complex. In general, CO2 captured by this method is of low purity, requiring multiple purification and less application in industry.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >The above CO2 capture technologies all have the disadvantages of high cost, low efficiency and poor circulability. These unavoidable disadvantages hinder their application in production and life. Therefore, new technologies are urgently needed, and the technology of carbonic anhydrase (CA) capture makes up for the shortage of other methods.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Carbonic anhydrase, a metal enzyme containing Zn2+, can catalyze CO2 and H2O to produce HCO3- (as shown in figure 1). Carbonic anhydride catalyzes faster than other types of enzymes. The range of carbonic anhydrase catalytic rates is 104 to 106 reactions per second. Among various sources of carbonic anhydrase, human carbonic anhydrase has the highest catalytic efficiency.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-21.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><img width="693" height="241" src="IGEM±³¾°½éÉÜ.files/IGEM±³¾°½éÉÜ5070.png" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:µÈÏß;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" > </span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Picture 1 Catalytic mechanism of carbonic anhydrase (CA2)</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;mso-char-indent-count:2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >The molecular weight of carbonic anhydrase is about 30KDa, which is composed of a single peptide chain and contains about 260 amino acids. Each enzyme molecule contains one Zn2+. The structure is ellipsoid, with a pouch cavity in the middle about 1.5nm deep and a cavity opening about 2.0nm wide. Zn2+ binds at the bottom of the cavity. At present, the most studied type of carbonic anhydrase is cosine-family CA, also known as CA2. Its main secondary structure is in its enzyme molecule 10 pali-fold. It is because of their existence that the enzyme structure is divided into two parts. Many key amino acid residues in enzyme molecules are related to their activity. In addition to extension-folding, the surface of the enzyme molecules is also distributed in the form of an icy-helical structure, which is usually a short structure (picture 2).</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><img width="567" height="426" src="IGEM±³¾°½éÉÜ.files/IGEM±³¾°½éÉÜ5981.png" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:µÈÏß;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Picture 2 The structure of carbonic anhydrase (CA2)</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Compared with other methods, carbonic anhydrase capture technology is specific and can capture CO2 from other gases. In addition, this method is environmentally friendly. Carbonic anhydrase converts CO2 into bicarbonate, which can meet the growth demand of plants and microorganisms. When CO2 is converted to bicarbonate, bicarbonate can combine with calcium ions to form calcium carbonate, which is stably stored underground.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Compared with other methods, carbonic anhydrase capture technology is more efficient. The main rate-limiting step of general CO2 capture technology is the hydration reaction of CO2, while carbonic anhydrase can significantly increase the hydration reaction rate of CO2, thus improving the CO2 capture efficiency.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >Although carbonic anhydrase capture technology has high efficiency, it still has some limitations. Because most natural carbonic anhydrase environmental sensitivity in the reaction, and do not have heat stability, carbonic anhydrase in CO2 capture, however, the environment temperature is 65 ℃, and natural carbonic anhydrase in the temperature cannot remain stable, after many times circulation loss of enzyme activity. The price of carbonic anhydrase is relatively expensive, and frequent replacement will greatly increase the cost of capture, which limits the wide spread of carbonic anhydrase capture technology. So the key now is to look for the thermal stability of carbonic anhydrase.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >In order to find the high efficiency catalysis and high stability of carbonic anhydrase, in this project, we use the high efficiency catalysis characteristic of human carbonic anhydrase 2 (hereinafter referred to as CA2), use molecular simulation method to optimize its amino acid sequence, and design the CA2 mutant with high activity and high stability. The project includes the following aspects:</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >1) molecular simulation;</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >2) construction of escherichia coli strains expressing wild-type and mutant CA2;</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >3) expression and purification of CA2;</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >4) practical application of CA2: CO2 capture.</span><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >1. Rahman F A, Aziz M M A, Saidur R, et al. Pollution to solution: Capture and sequestration of carbon dioxide (CO2) and its utilization as a renewable energy source for a sustainable future[J]. Renewable & Sustainable Energy Reviews, 2017, 71:112-126.</span><span style="mso-spacerun:'yes';font-family:µÈÏß;mso-bidi-font-family:ËÎÌå;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >2. Claudiu T. Supuran. Structure and function of carbonic anhydrases[J]. Biomolecular Biochemical journal, 2016, 473(14):2023-2032.</span><span style="mso-spacerun:'yes';font-family:µÈÏß;mso-bidi-font-family:ËÎÌå;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >3. Lionetto M G, Caricato R, Erroi E, et al. Potential application of carbonic anhydrase activity in bioassay and biomarker studies [J]. Chemistry & Ecology, 2006, 22(sup1): S119-S25.</span><span style="mso-spacerun:'yes';font-family:µÈÏß;mso-bidi-font-family:ËÎÌå;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="margin-left:24.0000pt;text-indent:-24.0000pt;mso-char-indent-count:-2.0000;line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" >4. Migliardini F, De L V, Carginale V, et al. Biomimetic CO2 capture using a highly thermostable bacterial α-carbonic anhydrase immobilized on a polyurethane foam [J]. Journal of Enzyme Inhibition & Medicinal Chemistry, 2014, 29(1): 146.</span><span style="mso-spacerun:'yes';font-family:µÈÏß;mso-bidi-font-family:ËÎÌå;font-size:10.5000pt;mso-font-kerning:1.0000pt;" ><o:p></o:p></span></p><p class=MsoNormal style="line-height:150%;" ><span style="mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:ËÎÌå;line-height:150%;font-size:12.0000pt;mso-font-kerning:1.0000pt;" ><o:p> </o:p></span></p></div><!--EndFragment--></html>
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