Difference between revisions of "Team:Edinburgh OG/Parts"

Revision as of 17:56, 16 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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       	 <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
       	 <td>1459bp</td>
-      	 <td>Original Sequence obtained from: NCBI(AM260479.1). Codon Optimised for E.coli</td>
+      	 <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> Codon Optimised for E.coli</td>NCBI(AM260479.1). Codon Optimised for E.coli</td>
        </tr>
        <tr>
 	      <td><a href="http://parts.igem.org/Part:BBa_K2739006">BBa_K2739006</a></td>
 	      <td>proR-PhaR-proP-PhaP-HlyA</td>

Registry Number	Brief Description	Key Details	Length/th>	Sequence information
BBa_K2739005	proR-PhaR-Pha promoter-PhaP	The phasin autoregulation system with its native promoter + phasin with its native promoter	1459bp	Original Sequence obtained from: NCBI(AM260479.1) Codon Optimised for E.coli
BBa_K2739006	proR-PhaR-proP-PhaP-HlyA	The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter	1646bp	Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501. Codon Optimised for E.coli
BBa_K2739007	LacP-RBS-PhaP-HlyA-Terminator	Phasin with HlyA signal peptide under the control of Lac promoter	1139bp	This part is developed from BBa_K390501
BBa_K2739009	Hybrid promoter-PhaCAB-Bktb	Co-expression of Bktb with PHA operon	5462bp	The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051
BBa_K2739010	Hybrid promoter-PhaCB-Bktb	Bktb in replacing the phaA in PHA operon	4252bp	The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCB is developed from BBa_K1149051