Difference between revisions of "Team:Vilnius-Lithuania/InterLab"

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         <h2>What is SynORI?</h2>
 
         <h2>What is SynORI?</h2>
         <p>SynORI stands for synthetic origin of replication. It is a framework designed to make working with single
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         <p>At the beginning of the InterLab study we completed three distinct calibration protocols. At first, we performed the LUDOX Protocol in order to obtain a conversion factor to transform absorbance (Abs600) from the plate reader into a comparable OD600 measurement as would be obtained with a spectrophotometer. Next, we completed the Microsphere Protocol as it allows a standard curve of particle concentration which is used to convert Abs600 measurements to an estimated number of cells. Finally, by completing the Fluorescein Protocol we generated a standard fluorescence curve which is used to compare fluorescence output of different test devices. Completion of the calibrations ensured that we take cell measurements under the same conditions. It is worth mentioning that prior calibration, we prepared competent E. coli DH5-alpha cells and transformed them according to the standard transformation protocol. During all of the experiments we tested 8 plasmids: 2 controls and 6 test devices (Table 1). </p>
            and multi-plasmid systems precise, easy and on top of that - more functional.</p>
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         <p>Table 1. Parts received and tested during iGEM’s fifth InterLab Study</p>
         <p>The SynORI framework enables scientists to build a multi-plasmid system in a standardized manner by:</p>
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         <p> Table 1. </p>
         <ol>
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            <li>Selecting the number of plasmid groups</li>
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             <li>Choosing the copy number of each group</li>
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            <li>Picking the type of copy number control (specific to one group or regulating all of them at once).</li>
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        </ol>
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        </p>
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Revision as of 20:25, 16 October 2018

InterLab

Abstract

The goal of this year’s InterLab Study was to identify and minimize the sources of systematic variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of optical density (OD).

Participating in the fifth iGEM InterLab Study was a great opportunity to start this year’s competition as well as acquire some valuable knowledge which we implemented into practice during the project.

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