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| − | {{:Team:UiOslo_Norway/Head}}
| + | https://2018.igem.org/Team:UiOslo_Norway |
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| − | <img src="https://static.igem.org/mediawiki/2018/7/7f/T--UiOslo_Norway--Canditect.png" class="front">
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| − | <h3 style="text-align:center;">Fast detection of vulvovaginal <em>Candida albicans</em> </br>infections using CRISPR/dCas9</h3>
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| − | <img class="front" src="https://static.igem.org/mediawiki/2018/3/33/T--UiOslo_Norway--Concept.png" alt="Concept graphic">
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| − | <h3 style="text-align: center; margin-left: auto; margin-right: right;">UiOslo Norway</h3>
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| − | <p style="text-align:justify;"> During their lifetime 75 % of women will experience a <em>Candida albicans</em> (<em>C. albicans</em>) infection, one of the most common vulvovaginal yeast infections. Currently there are no fast methods to detect whether an infection is caused by <em>C. albicans</em> or not. In addition, women may purchase over-the-counter antimycotics without knowing the cause of their infection. This contributes to the rise of antimycotic resistance, making treatment of future infections more difficult.
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| − | <p style="text-align:justify;"><a href="https://2018.igem.org/Team:UiOslo_Norway/Attributions#inspiration">Based on previous projects</a>, UiOslo Norway aims to develop a fast detection kit for <em>C. albicans</em> infections, using CRISPR/dCas9. Upon a suspected infection, a vaginal sample will be treated with glucanase to selectively lyse yeast cells walls, exposing the fungal DNA. Afterwards, modified dCas9 enzymes fused with split β-lactamase are added. Using specifically designed guide RNAs, the dCas9 complexes bind adjacently on <em>C. albicans</em> specific DNA sequences. This activates the β-lactamase to cleave its substrate nitrocefin, producing a colored product indicating the presence of <em>C. albicans</em> DNA.
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| − | <a href="https://www.uio.no/english/">
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| − | <img class="logo" src="https://static.igem.org/mediawiki/2018/3/32/T--UiOslo_Norway--UiO.png" target="_blank" alt="University of Oslo">
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| − | </a>
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| − | <a href="https://eu.idtdna.com/pages">
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| − | <img class="logo" src="https://static.igem.org/mediawiki/2018/a/ae/T--UiOslo_Norway--IDT.png" target="_blank" alt="Integrated DNA Technologies">
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| − | </a>
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| − | <a href="https://twistbioscience.com/">
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| − | <img class="logo" src="https://static.igem.org/mediawiki/2018/e/ea/T--UiOslo_Norway--Twist.png" target="_blank" alt="Twist Biosciences">
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| − | <a href="https://digitallifenorway.org/gb/">
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| − | <img class="logo" src="https://static.igem.org/mediawiki/2018/2/2a/T--UiOslo_Norway--CDL.png" target="_blank" alt="Centre for Digital Life Norway">
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| − | </a>
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| − | <a href="https://www.biokjemisk.no/">
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| − | <img class="logo" src="https://static.igem.org/mediawiki/2018/e/e2/T--UiOslo_Norway--NBS.png" target="_blank" alt="Norwegian Biochemical Society">
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| − | </a>
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| − | <a href="https://nordicbiosite.com/">
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| − | <img class="logo" src="https://static.igem.org/mediawiki/2018/b/b0/T--UiOslo_Norway--biosite.png" target="_blank" alt="Nordic BioSite">
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| − | </a>
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| − | <a href="https://sharelab.no">
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| − | <img class="logo" style="background-color: #1f374a; "src="https://static.igem.org/mediawiki/2018/8/87/T--UiOslo_Norway--sharelab.png" target="_blank" alt="Sharelab">
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