Difference between revisions of "Team:WLC-Milwaukee/Notebook"
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| − | + | Based on Judge feedback and our desire to bring the 2017 WLC-Milwaukee project to fruition, we decided to continue our 2017 project detecting <i>E. coli</i> in contaminated water supplies using Lambda Phage tail proteins. We began laying out the ways we would improve our past project and complete the areas where it had fallen short and been unsuccessful in 2017. | |
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| − | + | With the help of our advisor, we designed primers and began the frustrating process of amplifying and cloning our parts from the pETail4 plasmid (containing the Lambda Phage tail protein genes) into pTrc99a in order to control protein expression levels using the pTrc99a IPTG inducible trc-promoter (also called Ptac). | |
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| − | + | A number of our team members participated in MUBRC (The Milwaukee Undergraduate Biological Research Conference) by volunteering and helping facilitate setup for this event while Jack presented a poster of our past work and hopeful project improvements in order to gain feedback from other undergraduate researchers and PIs in attendance. | |
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Revision as of 21:44, 16 October 2018
Project Timeline
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Project Decisions!
February 2018
Based on Judge feedback and our desire to bring the 2017 WLC-Milwaukee project to fruition, we decided to continue our 2017 project detecting E. coli in contaminated water supplies using Lambda Phage tail proteins. We began laying out the ways we would improve our past project and complete the areas where it had fallen short and been unsuccessful in 2017.
Began Cloning parts into pTrc99a
March 2018
With the help of our advisor, we designed primers and began the frustrating process of amplifying and cloning our parts from the pETail4 plasmid (containing the Lambda Phage tail protein genes) into pTrc99a in order to control protein expression levels using the pTrc99a IPTG inducible trc-promoter (also called Ptac).
MUBRC
April 2018
A number of our team members participated in MUBRC (The Milwaukee Undergraduate Biological Research Conference) by volunteering and helping facilitate setup for this event while Jack presented a poster of our past work and hopeful project improvements in order to gain feedback from other undergraduate researchers and PIs in attendance.
Started Human Practices Investigation
June 2018
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Organization Outreach Day
August 2018
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Confirmed Clones in pTrc99a
September 2018
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Science Exploration Day
September 2018
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Protein Purification and Analysis
October 2018
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System Prototyping and Continued Testing
October 2018
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Young Women in Science Program
March 3rd 2018
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Planned Outreach Events
April 2018
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New Cloning Primers
May 2018
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Jack Tours Cornell Lab Spaces
August 2018
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Spoke with Experts to Analyze our System
September 2018
Modified our project design, usability, and scope of its application based on feedback and re-analysis of our system.
Tested HRP and Substrates based on new system goals
September 2018
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Obtained clones in pSB1C3
October 2018
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Visited MMSD treatment plant
October 2018
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Submitted Parts and finished the Wiki!
October 2018
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