Difference between revisions of "Team:UPF CRG Barcelona/Basic"
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DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene | DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene | ||
which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020). </p> | which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020). </p> | ||
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<p><b>BBa_K2581015: FadL</b></p> | <p><b>BBa_K2581015: FadL</b></p> | ||
<p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | <p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | ||
DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the transmembrane protein FadL. This | DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the transmembrane protein FadL. This | ||
gene has an essential role in the fatty acid transportation across the cell membrane via a transportation.</p> | gene has an essential role in the fatty acid transportation across the cell membrane via a transportation.</p> | ||
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<p><b>Bba_K2581016: FadD</b></p> | <p><b>Bba_K2581016: FadD</b></p> | ||
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This gene has an essential role in the fatty acid transportation inside the cell and in acyl-activation | This gene has an essential role in the fatty acid transportation inside the cell and in acyl-activation | ||
mechanism.</p> | mechanism.</p> | ||
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<p><b>BBa_K2581020: pAR</b></p> | <p><b>BBa_K2581020: pAR</b></p> | ||
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comparison with the existing part. | comparison with the existing part. | ||
</p> | </p> | ||
| − | <center><img src="/ | + | <center><img src="https://static.igem.org/mediawiki/2018/5/5b/T--UPF_CRG_Barcelona--pARbasic.svg" style="width: 35%;"></center> |
Revision as of 22:12, 16 October 2018
BASIC PARTS
BBa_K2581014: FadE
The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020).
BBa_K2581015: FadL
The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the transmembrane protein FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation.
Bba_K2581016: FadD
The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the acyl-coA synthetase gene FadD. This gene has an essential role in the fatty acid transportation inside the cell and in acyl-activation mechanism.
BBa_K2581020: pAR
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). This new promoter has two binding sites for FadR in comparison with the existing part.
