Difference between revisions of "Team:UPF CRG Barcelona/Improve"

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{{UPF_CRG_Barcelona}}
 
 
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<div class="clear"></div>
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<link rel="stylesheet" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/styles?action=raw&ctype=text/css" type='text/css'>
  
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<body>
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      <div class="container">
  
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<div class="column full_size">
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        <div class="navbar-brand">
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<h1>Improve</h1>
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          <a class="navbar-logo" href="#">
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
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            <img src="assets/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018">
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          </a>
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          <div class="navbar-burger" data-target="navbar-menu">
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            <span></span>
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            <span></span>
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            <span></span>
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          </div>
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        </div>
  
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<h3>Gold Medal Criterion #2</h3>
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        <!-- children of navbar-menu must be navbar-start and/or navbar-end -->
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement. Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
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        <div class="navbar-menu" id="navbar-menu">
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          <!-- navbar items -->
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          <div class="navbar-grid">
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            <!--class is-active for showing active menu-->
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            <a class="navbar-item" href="Description.html">Project</a>
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            <a class="navbar-item" href="DryLab.html">DryLab</a>
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            <a class="navbar-item is-active" href="Parts.html">Parts</a>
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            <a class="navbar-item" href="Deliverables.html">Deliverables</a>
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            <a class="navbar-item" href="WetLab.html">WetLab</a>
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            <a class="navbar-item" href="Intro_HP.html">Human Practices</a>
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            <a class="navbar-item" href="Aboutteam.html">Team</a>
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          </div>
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        </div>
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      </div>
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    </nav>
  
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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    <header id="header">
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      <div id="blur"></div>
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      <!-- Swiper -->
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      <div class="swiper-container" id="sw-container">
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        <div class="swiper-wrapper">
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          <a class="swiper-slide swiper-slide-menu" href="Basic.html">
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            <embed src="assets/T--UPF_CRG_Barcelona--basicpart.svg" alt="">
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            <p>Basic Parts</p>
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          </a>
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          <a class="swiper-slide swiper-slide-menu" href="Intermediate.html">
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            <img style="height: 50px" src="assets/T--UPF_CRG_Barcelona--compositepart.svg" alt="">
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            <p>Construction<br>Intermediates</p>
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          </a>
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          <a class="swiper-slide swiper-slide-menu" href="Composite.html">
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            <embed src="assets/T--UPF_CRG_Barcelona--compositepart.svg" alt="">
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            <p>Composite Parts</p>
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          </a>
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          <a class="swiper-slide swiper-slide-menu" href="Improve.html">
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            <embed src="assets--UPF_CRG_Barcelona--improvedpart_1.svg" alt="">
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            <p>Improved Parts</p>
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          </a>
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          <a class="swiper-slide swiper-slide-menu" href="Registry.html">
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            <embed src="assets/T--UPF_CRG_Barcelona--registryparts.svg" alt="">
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            <p>Parts Registry</p>
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          </a>
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        </div>
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        <!-- Add Arrows -->
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    </header>
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<br><br>
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  <main>
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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    <section class="">
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      <div>
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        <p class="page-title">IMPROVED PARTS</p>
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        <p><b>BBa_K2581011: T14_pAR2_RFP or Improved fatty acid acyl-CoA biosensor with weak RBS CANVIAR!!!</b></p>
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        <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty
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          acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA
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          but in this case the high basality activity of the promoter has been reduced. </p>
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        <p>This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator
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          (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS
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          (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from <i>Discosoma striata</i>
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          (BBa_E1010).
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        </p>
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        <center><img src="/assets/T--UPF_CRG_Barcelona--goldenpart32.svg" style="width: 50%;"></center>
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        <p><b>BBa_K2581012: T14_pAR4_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS</b></p>
 +
        <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty
 +
          acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA
 +
          but in this case the high basality activity of the promoter has been reduced.</p>
 +
        <p>This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator
 +
          (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS
 +
          (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from <i>Discosoma striata</i>
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          (BBa_E1010). </p>
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        <center><img src="/assets/T--UPF_CRG_Barcelona--goldenpart34.svg" style="width: 50%;"></center>
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      </div>
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    </section>
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    <div class="spacer"></div>
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  </main>
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</html>
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{{UPF_CRG_Barcelona}}
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Revision as of 22:32, 16 October 2018

IMPROVED PARTS

BBa_K2581011: T14_pAR2_RFP or Improved fatty acid acyl-CoA biosensor with weak RBS CANVIAR!!!

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.

This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).

BBa_K2581012: T14_pAR4_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.

This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).

Wiki