Difference between revisions of "Team:Warwick/Description"

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                         $("#TB2P").html("Citations<br><br>Jobling S, Burn RW, Thorpe K, Williams R, Tyler C. Statistical Modeling Suggests that Antiandrogens in Effluents from Wastewater Treatment Works Contribute to Widespread Sexual Disruption in Fish Living in English Rivers. Environmental Health Perspectives. 2009;117(5):797-802. doi:10.1289/ehp.0800197<br><br>Jobling S, Owen R. Ethinyl oestradiol in the aquatic environment. Copenhagen, Denmark: European Environment Agency 2013. Report No.: No. 1, Vol. 13.");
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                         $("#TB2P").html("Citations<br><br>Jobling S, Burn RW, Thorpe K, Williams R, Tyler C. Statistical Modeling Suggests that Antiandrogens in Effluents from Wastewater Treatment Works Contribute to Widespread Sexual Disruption in Fish Living in English Rivers. Environmental Health Perspectives. 2009;117(5):797-802. doi:10.1289/ehp.0800197<br>Jobling S, Owen R. Ethinyl oestradiol in the aquatic environment. Copenhagen, Denmark: European Environment Agency 2013. Report No.: No. 1, Vol. 13.");
 
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                         $("#TB2P").html("Citations Contd.<br><br>JSchwindt, A. R., Winkelman, D. L., Keteles, K. , Murphy, M. , Vajda, A. M. and Frid, C. (2014), An environmental oestrogen disrupts fish population dynamics through direct and transgenerational effects on survival and fecundity. J Appl Ecol, 51: 582-591<br><br>Yi-Lung Chen, Chang-Ping Yu, Tzong-Huei Lee, King-Siang Goh, Kung-Hui Chu, Po-Hsiang Wang, Wael Ismail, Chao-Jen Shih, Yin-Ru Chiang, Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways,Cell Chemical Biology,Volume 24, Issue 6,2017,Pages 712-724.e7");
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                         $("#TB2P").html("Citations Contd.<br><br>JSchwindt, A. R., Winkelman, D. L., Keteles, K. , Murphy, M. , Vajda, A. M. and Frid, C. (2014), An environmental oestrogen disrupts fish population dynamics through direct and transgenerational effects on survival and fecundity. J Appl Ecol, 51: 582-591<br>Yi-Lung Chen, Chang-Ping Yu, Tzong-Huei Lee, King-Siang Goh, Kung-Hui Chu, Po-Hsiang Wang, Wael Ismail, Chao-Jen Shih, Yin-Ru Chiang, Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways,Cell Chemical Biology,Volume 24, Issue 6,2017,Pages 712-724.e7");
 
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Revision as of 23:51, 16 October 2018

Ripple


Project Description

Biological

We have developed a detection kit for Legionella Pneumophila - a pathogenic bacteria responsible for Legionnaires disease. The legionella family retain a highly conserved but unique protein known as Legiolysin (a hemolysin toxin). We developed a self-folding mRNA known as a riboswitch; the device only unfolds when binding specifically to a segment of the Legiolysin toxin. We then fused our riboswitch with the guide RNA of a type II Streptococcus pyogenes dCAS9 in order to block the targeting domain of the guide sequence with a double stranded RNA loop.

Organic

For the biological aspect of our water safety focus, we looked at the growing problem of high oestrogen levels in British waterways negatively impacting fish populations, and attempted to provide a solution using synthetic biology. The synthetic oestrogen, 17β-estradiol (EE2), is the form that has the largest effect on fish populations, and levels in British rivers range from around 0.05 to 2.80 ng L -1 (Jobling et al, 2009). This is the form of oestrogen we decided to target with our project. This may seem like a very small amount, however studies have shown that even extremely low concentrations such as these can have profound effects on freshwater fish.

Inorganic

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