Difference between revisions of "Team:NDC-HighRiverAB/Parts"
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<li>First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa.<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2694001">(BBa_K2694001)</a></li> | <li>First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa.<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2694001">(BBa_K2694001)</a></li> | ||
<li>For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli.<a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a></li> | <li>For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli.<a href="http://parts.igem.org/Part:BBa_R0011">(Part:BBa_B0011)</a></li> | ||
| − | <li>The RBS we used is B0034. (Part: BBa_B0034) </li> | + | <li>The RBS we used is B0034. <a href="http://parts.igem.org/Part:BBa_B0034">(Part:BBa_B0034)</a></li> |
<li>Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part: BBa_B0015) </li> | <li>Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part: BBa_B0015) </li> | ||
<li>After we had chosen all of our biobrick parts, we combined them into the plasmid <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a></li> | <li>After we had chosen all of our biobrick parts, we combined them into the plasmid <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a></li> | ||
Revision as of 02:21, 17 October 2018
Parts
This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (Part:BBa_B0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase: