Difference between revisions of "Team:Makerere University"

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{{Makerere_University}}
 
 
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<title>Project description</title>
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<h1> Welcome to iGEM 2018! </h1>
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<ul>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<li><a  href="https://2018.igem.org/Team:Makerere_University/home.html">Home</a></li>
  
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<img src="http://placehold.it/1080x320/c4baba/e4dede">
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<li><a class="active" href="https://2018.igem.org/Team:Makerere_University/Description" >Project
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<ul class="submenus">
  
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<li><a class="active" href="https://2018.igem.org/Team:Makerere_University/description">description</a></li>
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<li><a href="design.html">design</a></li>
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<li><a href="notebook.html">notebook</a></li>
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<li><a href="results.html">results</a></li></a>
  
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<h3>Before you start</h3>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
 
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
 
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</li>
  
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<li><a href="members.html" >Team
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<li><a href="members.html" >Team members</a></li>
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<li><a href="collaborations.html" >Team collaborations</a></li></a>
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</ul>
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</li>
  
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<li><a href="parts.html" >Parts</a></li>
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<li><a href="safety.html" >Safety</a></li>
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<li><a href="human practices.html" >Humanpractices</a></li>
  
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h3> Uploading pictures and files </h3>
 
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
 
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
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<a href="https://2018.igem.org/Special:Upload">
 
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UPLOAD FILES
 
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<div id="content">
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<h1>Project description</h1>
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<p>The project is set up to utilize the enzymatic machinery from the bacterium <b><i>Ideonella sakaiensis</i></b> as it is capable of manufacturing the enzymes of <i>PETase</i> and <i>MHETase</i> which are important in the degradation of PET. <br>
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<h3> Wiki template information </h3>
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Isolated segments of the genes for the respective enzymes are transformed into the a competent <i>E. coli</i> cell so that it also obtains the plastic degrading character as in <i>Ideonella</i>.
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<i>Ideonella sakaiensis</i> is a bacteria that naturally decomposes polyethylene terephthalate, we decide to genetically modify E. coli cells to model the plastic degradation machine by adding the Lipase and Chlorogenate Esterase genes from Ideonella sakaiensis into E. coli bacterial cells. We shall obtain the two genes encoding the enzymes used by Ideonella sakaiensis, PETase and MHETase, and insert the gene into E. coli plasmids and then put the plasmids into E. coli cells. With the engineered E.coli bacteria, the enzymes produced are able to express the plastic-degrading abilities
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<b>Goal:</b> To have a clean and safe environment free of plastic pollution.
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<h3> Editing your wiki </h3>
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<b>Objectives.</b>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<li>To engineer a bacteria capable of degrading PET plastics.</li>
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<li>To sensitize the community about the danger associated with plastic wastes.</li>
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</ol>
  
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<a href="https://2018.igem.org/wiki/index.php?title=Team:Makerere_University&action=edit">
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EDIT PAGE
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#content{
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margin: 0;
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background:yellow;
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background:transparent;
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text-align:center;
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#mainlinks ul li{
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text-align:center;
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border:1px solid white;
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margin-top: 2px;
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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background: white;
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Revision as of 13:16, 28 June 2018

Project description

Project description

The project is set up to utilize the enzymatic machinery from the bacterium Ideonella sakaiensis as it is capable of manufacturing the enzymes of PETase and MHETase which are important in the degradation of PET.
Isolated segments of the genes for the respective enzymes are transformed into the a competent E. coli cell so that it also obtains the plastic degrading character as in Ideonella.

Ideonella sakaiensis is a bacteria that naturally decomposes polyethylene terephthalate, we decide to genetically modify E. coli cells to model the plastic degradation machine by adding the Lipase and Chlorogenate Esterase genes from Ideonella sakaiensis into E. coli bacterial cells. We shall obtain the two genes encoding the enzymes used by Ideonella sakaiensis, PETase and MHETase, and insert the gene into E. coli plasmids and then put the plasmids into E. coli cells. With the engineered E.coli bacteria, the enzymes produced are able to express the plastic-degrading abilities

Goal: To have a clean and safe environment free of plastic pollution.

Objectives.

  1. To engineer a bacteria capable of degrading PET plastics.
  2. To sensitize the community about the danger associated with plastic wastes.