Difference between revisions of "Team:ZJU-China/Notebook"

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<div class="title">
 
<div class="title">
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<div class="tl">MODEL&nbsp;&nbsp;</div>
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<div class="tl">LABEL&nbsp;&nbsp;</div>
 
</div>
 
</div>
 
<div class="intro">
 
<div class="intro">
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<div class="psg" >
 
<div class="psg" >
 
<span class="psg_ttl">Lab Book</span>
 
<span class="psg_ttl">Lab Book</span>
−
<section class="cd-horizontal-timeline" style="border:1px solid black; height: 80% !important;">
+
<section class="cd-horizontal-timeline" style="height: 120% !important;">
 
<div class="timeline">
 
<div class="timeline">
 
<div class="events-wrapper">
 
<div class="events-wrapper">
 
<div class="events">
 
<div class="events">
 
<ol>
 
<ol>
−
<li><a href="#0" data-date="31/12/2010" class="selected">Dec. 31</a></li>
+
<li><a href="#0" data-date="31/12/2010" class="selected">End Of Winter</a></li>
−
<li><a href="#0" data-date="30/04/2011">Mar.8 - Jun.2</a></li>
+
<li><a href="#0" data-date="30/07/2011">Mar.8 - Jun.2</a></li>
−
<li><a href="#0" data-date="31/09/2011">Jun. 23</a></li>
+
<li><a href="#0" data-date="31/11/2011">Jun. 15</a></li>
−
<li><a href="#0" data-date="31/01/2012">Jun. 25</a></li>
+
<li><a href="#0" data-date="31/01/2012">Jun. 23</a></li>
−
<li><a href="#0" data-date="30/06/2012">Jul. 13</a></li>
+
<li><a href="#0" data-date="30/04/2012">Jun. 25</a></li>
−
<li><a href="#0" data-date="31/12/2012">Jul. 25</a></li>
+
<li><a href="#0" data-date="31/01/2013">Jul. 13</a></li>
−
<li><a href="#0" data-date="31/05/2013">Jul. 29</a></li>
+
<li><a href="#0" data-date="30/06/2013">Jul. 25</a></li>
−
<li><a href="#0" data-date="31/12/2013">Aug. 14</a></li>
+
<li><a href="#0" data-date="30/08/2013">Jul. 29</a></li>
−
<li><a href="#0" data-date="10/12/2018">Aug. 18</a></li>
+
<li><a href="#0" data-date="31/01/2014">Aug. 6</a></li>
−
<li><a href="#0" data-date="19/01/2019">Aug. 25</a></li>
+
<li><a href="#0" data-date="30/04/2014">Aug. 14</a></li>
−
<li><a href="#0" data-date="03/03/2020">Aug. 26</a></li>
+
<li><a href="#0" data-date="31/06/2014">Aug. 18</a></li>
−
<li><a href="#0" data-date="04/03/2021">Sep. 3</a></li>
+
<li><a href="#0" data-date="31/11/2014">Aug. 25</a></li>
−
<li><a href="#0" data-date="05/03/2022">Sep. 25</a></li>
+
<li><a href="#0" data-date="31/12/2014">Aug. 26</a></li>
−
<li><a href="#0" data-date="11/03/2023">Aug. 1 - 15</a></li>
+
<li><a href="#0" data-date="31/05/2015">Sep. 3</a></li>
 +
<li><a href="#0" data-date="31/12/2015">Sep. 25</a></li>
 +
<li><a href="#0" data-date="31/05/2016">Oct. 1 - 15</a></li>
 
</ol>
 
</ol>
  
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<div class="events-content">
 
<div class="events-content">
 
<ol>
 
<ol>
−
<li class="selected" data-date="31/12/2010">
+
<li style="text-align: left;"class="selected" data-date="31/12/2010">
−
<h2>Horizontal Timeline</h2>
+
<h2>Our Story</h2>
−
<em>January 16th, 2014</em>
+
<em>End of Winter</em>
−
<p>
+
<p>After the end of the winter selection, we formed a new team - ZJU-China 2018. It is the beginning of the story.</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/5/5e/T--ZJU-China--notebook1.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="30/04/2011">
+
<li data-date="30/07/2011">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>February 28th, 2014</em>
+
<em>March 8 - June 2</em>
−
<p>
+
<p style="font-size: .9em !important;">At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex</p>
−
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+
<p style="font-size: .9em !important;"></br>At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex!</p>
−
</p>
+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/7/7f/T--ZJU-China--notebook2.jpg" />
 
</li>
 
</li>
  
−
<li data-date="31/09/2011">
+
<li data-date="31/11/2011">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>March 20th, 2014</em>
+
<em>June 15</em>
−
<p>
+
<p>We went out to play and know each other better!</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/8/84/T--ZJU-China--notebook3.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="30/01/2012">
+
<li data-date="31/01/2012">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>May 20th, 2014</em>
+
<em>June 23</em>
−
<p>
+
<p>We officially confirmed the project as a protein scaffold and started the experiment.</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/d/dc/T--ZJU-China--notebook4.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="30/06/2012">
+
<li data-date="30/04/2012">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>July 9th, 2014</em>
+
<em>June 25</em>
−
<p>
+
<p>We divided our project into three parts. 1 - Curli expression. 2 - Enzyme construction 3 - Electrode modification. We prepared essential material and protocols for the the most important battlefield - Summer!</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/e/e2/T--ZJU-China--notebook5.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="31/12/2012">
+
<li data-date="31/01/2013">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>August 30th, 2014</em>
+
<em>July 13</em>
−
<p>
+
<p>The summer vacation had begun. The csgA protein has been successfully expressed. However, the construction of csgA KO strains met some difficulties. Despite this, the staged results still make us very happy!</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/a/a0/T--ZJU-China--notebook6.jpg" />
−
</p>
+
 
</li>
 
</li>
−
 
+
<li data-date="30/06/2013">
−
<li data-date="31/05/2013">
+
<h2>Our Story</h2>
−
<h2>Event title here</h2>
+
<em>July 25</em>
−
<em>September 15th, 2014</em>
+
<p>We have successfully constructed recombination strains with exogenous protein. Since the expression of eukaryotic proteins in prokaryotic systems is indeed a challenge, we have many failures. However, after many attempts, we successfully completed the expected goal!</p>
−
<p>
+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/8/81/T--ZJU-China--notebook7.jpg" />
−
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+
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="31/12/2013">
+
<li data-date="30/08/2013">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>November 1st, 2014</em>
+
<em>July 29</em>
−
<p>
+
<p>We had a pleasant meeting with ACON Bio. After showing them the project, they feel that our project has great possibilities, but it needs to be improved in some practical aspects.</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/1/12/T--ZJU-China--notebook8.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="10/12/2018">
+
<li data-date="31/01/2014">
−
<h2>Event title here</h2>
+
<h2>Our Story</h2>
−
<em>December 10th, 2014</em>
+
<em>August 6</em>
−
<p>
+
<p>We went to the Zhejiang Science and Technology Museum and hoped to promote our own projects. In a cheerful atmosphere, we not only introduced them to the basic knowledge of synthetic biology, but also introduced some of our projects. To our surprise, the children have a very strong interest in our projects. A child even pointed out a problem that we ourselves are confused! What a surprising experience.</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/d/d2/T--ZJU-China--notebook9.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="19/01/2019">
+
<li data-date="30/04/2014">
−
<h2>Event title here</h2>
+
<h2> Our Story</h2>
−
<em>January 19th, 2015</em>
+
<em>August 14</em>
−
<p>
+
<p style="font-size: .9em !important;">We have learned the electrode technology and decided to make two products - one is basic, using electrodes as well as electrochemical workstations, the other is smaller, and can even in a pocket.</p>
−
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+
<p style="font-size: .9em !important;"></br>Work on 2D printing is also progressing well. We made a practical 2D printer and prepared to use it to accurately print the enzyme onto the electrode.</p>
−
</p>
+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/1/1e/T--ZJU-China--notebook10.jpg" />
 
</li>
 
</li>
  
−
<li data-date="03/03/2020">
+
<li data-date="31/06/2014">
−
<h2>Event title here</h2>
+
<h2> Our Story</h2>
−
<em>March 3rd, 2015</em>
+
<em>August 18</em>
−
<p>
+
<p>We had sought help from Shanghai Tech University and gotten a csgA-KO strain. With this bacterium, our project has taken a big step forward. By testing the KO strain, we verified the function of Curli and prepared to fix it to the electrode in the next step.</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/2/22/T--ZJU-China--notebook11.jpg" />
−
</p>
+
 
</li>
 
</li>
  
−
<li data-date="04/03/2021">
+
<li data-date="31/11/2014">
−
<h2>Event title here</h2>
+
<h2> Our Story</h2>
−
<em>March 3rd, 2015</em>
+
<em>August 25</em>
−
<p>
+
<p>We went to CCIC. This is the Asian Forum on Synthetic Biology, and we showcased our projects. Other teams in china gave us many useful suggestions and had a lot of communication with us. Through CCIC, we knew everyone's progress and also clarified the subsequent tasks.</p>
−
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<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/5/56/T--ZJU-China--notebook12.jpg" />
−
</p>
+
</li>
 +
 
 +
<li data-date="31/12/2014">
 +
<h2> Our Story</h2>
 +
<em>August 26</em>
 +
<p>We have consulted some question about safety with Joichim messing，he gave us a lot of practical advises. Thanks so much for him!  </p>
 +
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/9/90/T--ZJU-China--notebook13.jpg" />
 
</li>
 
</li>
  
−
<li data-date="05/03/2022">
+
<li data-date="31/05/2015">
−
<h2>Event title here</h2>
+
<h2> Our Story</h2>
−
<em>March 3rd, 2015</em>
+
<em>September 3</em>
−
<p>
+
<p>We basically completed the production of the first generation of products. The results given by the electrochemical workstation are very satisfactory. However, its large size limits the practicality of the product. At the same time, the development of the second generation of smaller and lighter products is nearing completion. We are going to get the final result!</p>
−
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+
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/1/1e/T--ZJU-China--notebook14.jpg" />
−
</p>
+
</li>
 +
<li data-date="31/12/2015">
 +
<h2> Our Story</h2>
 +
<em>September 25</em>
 +
<p>We finally finished the final product! A beautiful interdigital electrode successfully distinguishes between the patient and the normal person. Although its stability needs to be further improved, this achievement is very exciting!</p>
 +
<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/c/cd/T--ZJU-China--notebook15.jpg" />
 
</li>
 
</li>
−
<li data-date="11/03/2023">
+
<li data-date="31/05/2016">
−
<h2>Event title here</h2>
+
<h2> Our Story</h2>
−
<em>March 3rd, 2015</em>
+
<em>August 1 – 15</em>
−
<p>
+
<p>After completing the final optimization of the product, we started writing wikis. Writing a wiki every day keeps doctor away.</p>
−
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<img style="width: 60% !important; margin-top: 2em; margin-left: 20%;" src="https://static.igem.org/mediawiki/2018/b/b9/T--ZJU-China--notebook16.jpg" />
−
</p>
+
 
</li>
 
</li>
  

Revision as of 05:36, 17 October 2018

HOME
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JUDGING FORM ⇗
Model
LABEL  


Lab Book
  1. End Of Winter
  2. Mar.8 - Jun.2
  3. Jun. 15
  4. Jun. 23
  5. Jun. 25
  6. Jul. 13
  7. Jul. 25
  8. Jul. 29
  9. Aug. 6
  10. Aug. 14
  11. Aug. 18
  12. Aug. 25
  13. Aug. 26
  14. Sep. 3
  15. Sep. 25
  16. Oct. 1 - 15

  1. Our Story

    End of Winter

    After the end of the winter selection, we formed a new team - ZJU-China 2018. It is the beginning of the story.

  2. Our Story

    March 8 - June 2

    At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex


    At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex!

  3. Our Story

    June 15

    We went out to play and know each other better!

  4. Our Story

    June 23

    We officially confirmed the project as a protein scaffold and started the experiment.

  5. Our Story

    June 25

    We divided our project into three parts. 1 - Curli expression. 2 - Enzyme construction 3 - Electrode modification. We prepared essential material and protocols for the the most important battlefield - Summer!

  6. Our Story

    July 13

    The summer vacation had begun. The csgA protein has been successfully expressed. However, the construction of csgA KO strains met some difficulties. Despite this, the staged results still make us very happy!

  7. Our Story

    July 25

    We have successfully constructed recombination strains with exogenous protein. Since the expression of eukaryotic proteins in prokaryotic systems is indeed a challenge, we have many failures. However, after many attempts, we successfully completed the expected goal!

  8. Our Story

    July 29

    We had a pleasant meeting with ACON Bio. After showing them the project, they feel that our project has great possibilities, but it needs to be improved in some practical aspects.

  9. Our Story

    August 6

    We went to the Zhejiang Science and Technology Museum and hoped to promote our own projects. In a cheerful atmosphere, we not only introduced them to the basic knowledge of synthetic biology, but also introduced some of our projects. To our surprise, the children have a very strong interest in our projects. A child even pointed out a problem that we ourselves are confused! What a surprising experience.

  10. Our Story

    August 14

    We have learned the electrode technology and decided to make two products - one is basic, using electrodes as well as electrochemical workstations, the other is smaller, and can even in a pocket.


    Work on 2D printing is also progressing well. We made a practical 2D printer and prepared to use it to accurately print the enzyme onto the electrode.

  11. Our Story

    August 18

    We had sought help from Shanghai Tech University and gotten a csgA-KO strain. With this bacterium, our project has taken a big step forward. By testing the KO strain, we verified the function of Curli and prepared to fix it to the electrode in the next step.

  12. Our Story

    August 25

    We went to CCIC. This is the Asian Forum on Synthetic Biology, and we showcased our projects. Other teams in china gave us many useful suggestions and had a lot of communication with us. Through CCIC, we knew everyone's progress and also clarified the subsequent tasks.

  13. Our Story

    August 26

    We have consulted some question about safety with Joichim messing，he gave us a lot of practical advises. Thanks so much for him!

  14. Our Story

    September 3

    We basically completed the production of the first generation of products. The results given by the electrochemical workstation are very satisfactory. However, its large size limits the practicality of the product. At the same time, the development of the second generation of smaller and lighter products is nearing completion. We are going to get the final result!

  15. Our Story

    September 25

    We finally finished the final product! A beautiful interdigital electrode successfully distinguishes between the patient and the normal person. Although its stability needs to be further improved, this achievement is very exciting!

  16. Our Story

    August 1 – 15

    After completing the final optimization of the product, we started writing wikis. Writing a wiki every day keeps doctor away.


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