Difference between revisions of "Team:ECUST/Protocol"
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<h1 class="box-heading">Basic techniques in Molecular biology</h1> | <h1 class="box-heading">Basic techniques in Molecular biology</h1> | ||
<h2>Polymerase chain reaction</h2> | <h2>Polymerase chain reaction</h2> | ||
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| − | <tr> | + | <tr><th>Total volume</th><th>50/μL</th></tr> |
| − | + | <tr><td>DDW</td><td>36</td></tr> | |
| − | + | <tr><td>10×Buffer</td><td>5</td></tr> | |
| − | + | <tr><td>dNTP（2.5mM）</td><td>5</td></tr> | |
| − | <tr> | + | <tr><td>FP（10μM）</td><td>1</td></tr> |
| − | + | <tr><td>RP（10μM）</td><td>1</td></tr> | |
| − | + | <tr><td>DNA template</td><td>1</td></tr> | |
| − | + | <tr><td>EasyPfu DNA Polymerase</td><td>1</td></tr> | |
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</table> | </table> | ||
</div> | </div> | ||
Revision as of 06:00, 17 October 2018