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Revision as of 06:20, 17 October 2018

Notebook

Basic techniques in Molecular biology

Polymerase chain reaction

Total volume50/μL
DDW36
10×Buffer5
dNTP(2.5mM)5
FP(10μM)1
RP(10μM)1
DNA template1
EasyPfu DNA Polymerase1

Thermocycling

The PCRmachine should be set to run the following steps

Pre-denaturation94℃5min
transsexual94℃30s30circles
annealingTm-5℃30s
extend72℃0.5kb/min
extend72℃5min