Difference between revisions of "Team:HZAU-China/Notebook"
| Line 789: | Line 789: | ||
<h2><b>1.</b> Verification of Poisson distribution</h2> | <h2><b>1.</b> Verification of Poisson distribution</h2> | ||
<p> | <p> | ||
| − | We infect tumor cells with Salmonella carrying the GFP gene for a period of time and then | + | We infect tumor cells with <i>Salmonella</i> carrying the GFP gene for a period of time and then |
| − | count, and the result is that the distribution of Salmonella in the tumor cells | + | count, and the result is that the distribution of <i>Salmonella</i> in the tumor cells |
is consistent with the Poisson distribution. | is consistent with the Poisson distribution. | ||
</p> | </p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | |
| − | <p><b>Figure 1.</b> Figure 5: Infection of Salmonella and the result and result of analysis. | + | <p><b>Figure 1.</b> Figure 5: Infection of <i>Salmonella</i> and the result and result of analysis. |
The amount of cells and bacteria is obtained through experiments. | The amount of cells and bacteria is obtained through experiments. | ||
</p><br><br> | </p><br><br> | ||
| − | <p>Cell density is | + | <p>Cell density is 1*10^5 cm<sup>2</sup>. |
The concentration of bacteria is converted according to MOI and cell density. | The concentration of bacteria is converted according to MOI and cell density. | ||
| − | We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is equal to 0.0003, the curve simulated by the mathematical model matches the experimental results. | + | We adjusted As to match the curve to the results of the <i>Salmonella</i> infection experiment. When As is equal to 0.0003, the curve simulated by the mathematical model matches the experimental results. |
</p> | </p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2018/8/84/T--HZAU-China--Notebook-fig2.png" width="100%" alt=""> | |
<p><b>Figure 2.</b> </p> | <p><b>Figure 2.</b> </p> | ||
Revision as of 07:55, 17 October 2018
1. Verification of Poisson distribution
We infect tumor cells with Salmonella carrying the GFP gene for a period of time and then count, and the result is that the distribution of Salmonella in the tumor cells is consistent with the Poisson distribution.
Figure 1. Figure 5: Infection of Salmonella and the result and result of analysis. The amount of cells and bacteria is obtained through experiments.
Cell density is 1*10^5 cm2. The concentration of bacteria is converted according to MOI and cell density. We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is equal to 0.0003, the curve simulated by the mathematical model matches the experimental results.
Figure 2.
