Difference between revisions of "Team:HZAU-China/Notebook"

Line 786: Line 786:
 
         <div class="zhengwen">
 
         <div class="zhengwen">
 
             <div id="float01" class="cur">
 
             <div id="float01" class="cur">
             <div class="h1">Processing of experimental results</div>
+
             <div class="h1">Processing of experiment results</div>
 
             <h2><b>1.</b> Verification of Poisson distribution</h2>  
 
             <h2><b>1.</b> Verification of Poisson distribution</h2>  
 
             <p>
 
             <p>
Line 794: Line 794:
 
             </p>   
 
             </p>   
 
             <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt="">
 
             <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt="">
                     <p><b>Figure 1.</b> Figure 5: Infection of <i>Salmonella</i> and the result and result of analysis.
+
                     <p><b>Figure 1.</b> Figure 5: Infection of <i>Salmonella</i> and the result and result of analysis.</p>
                         The amount of cells and bacteria is obtained through experiments.
+
                         <p>The amount of cells and bacteria is obtained through experiments.
 
                         </p><br><br>
 
                         </p><br><br>
  

Revision as of 07:56, 17 October 2018

Processing of experiment results

1. Verification of Poisson distribution

We infect tumor cells with Salmonella carrying the GFP gene for a period of time and then count, and the result is that the distribution of Salmonella in the tumor cells is consistent with the Poisson distribution.

Figure 1. Figure 5: Infection of Salmonella and the result and result of analysis.

The amount of cells and bacteria is obtained through experiments.



Cell density is 1*10^5 cm2. The concentration of bacteria is converted according to MOI and cell density. We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is equal to 0.0003, the curve simulated by the mathematical model matches the experimental results.

Figure 2.